Team:Toulouse/Project/Overviews

From 2014.igem.org

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Facing this emergency, the students from the iGEM Toulouse Team decided to be committed to the protection of their local heritage. Using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Using different genetic modules, we engineered a bacterium (SubtiTree) capable of heading towards the pathogen, binding to its cell wall and finally delivering different fungicides to save the tree from its invaders. Our team also began to design genetic modules preventing any accidental spreading of the optimized microorganism, thus limiting the ecological and ethical footprints of SubtiTree. Although our project originated from a very local and specific tree disease, it could be transposable to other plant diseases.
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Facing this emergency, the students from the iGEM Toulouse Team, who enjoy chilling in this peaceful place, decided to be committed to the protection of their local heritage. Using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Using different genetic modules, we engineered a bacterium (SubtiTree) capable of heading towards the pathogen, binding to its cell wall and finally delivering different fungicides to save the tree from its invaders. Our team also began to elaborate different strategies to prevent any accidental spreading of the optimized microorganism, thus limiting the ecological and ethical footprints of SubtiTree. Although our project originated from a very local and specific tree disease, it could be transposable to other plant diseases.
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<p class="title1">Choice of the chassis </p>
<p class="title1">Choice of the chassis </p>
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<p class="texte"><i>Bacillus subtilis</i> has been reported to be an endophyte bacterium of a large variety of plants and trees. Therefore, this model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer <i>Bacillus subtilis</i> to fight Canker Stain inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.</p>
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<p class="texte"><i>Bacillus subtilis</i> has been reported to be an endophyte bacterium of a large variety of plants and trees. Therefore, this model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer <i>Bacillus subtilis</i> to fight Canker Stain from the inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.</p>
<center><img style="width:700px; margin: -10px 0 55px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg"></center>
<center><img style="width:700px; margin: -10px 0 55px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg"></center>
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<p class="title1">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="title1">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="texte">In the third step, our designed bacterium fights the pathogen by production of a powerful treatment based on the release of three different fungicides.</p>
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<p class="texte">In the third step, our designed bacterium fights the pathogen by producing a powerful treatment of three different fungicides.</p>
<p class="title1">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
<p class="title1">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
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<p class="texte">Our team worked on different aspects to control SubtiTree released in the environment. The aim is to prevent horizontal transfers between different bacteria and limit the growth and survival of the engineered bacterium inside the tree during one season.</p>
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<p class="texte">Our team worked on different aspects to control SubtiTree's spreading. The aim is to prevent horizontal transfers between different bacteria and to limit the growth and the survival of the engineered bacterium inside the tree during one season.</p>
        
        

Latest revision as of 19:57, 17 October 2014