Team:Toulouse/Project/Overviews

From 2014.igem.org

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<img style="width:700px; margin: 45px 0 45px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg">
<img style="width:700px; margin: 45px 0 45px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg">
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<p class="title2">Chemotaxis<a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="title1">Chemotaxis<a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="texte">First, the bacterium targets the pathogen thanks to a chemotaxis module which recognizes the soluble chitin monomers (N-acetyl-glucosamine) released by the fungi.</p>
<p class="texte">First, the bacterium targets the pathogen thanks to a chemotaxis module which recognizes the soluble chitin monomers (N-acetyl-glucosamine) released by the fungi.</p>
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<p class="title2">Binding<a href="http://2014.igem.org/Team:Toulouse/Project/Binding"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="title1">Binding<a href="http://2014.igem.org/Team:Toulouse/Project/Binding"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="texte">Then, SubtiTree binds onto the pathogen by a chimeric protein anchored to the bacterium peptidoglycan and which can make a bridge between bacterial cell wall and fungal chitin, the main component of the pathogen's cell wall.</p>
<p class="texte">Then, SubtiTree binds onto the pathogen by a chimeric protein anchored to the bacterium peptidoglycan and which can make a bridge between bacterial cell wall and fungal chitin, the main component of the pathogen's cell wall.</p>
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<p class="title2">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="title1">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="texte">To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.</p>
<p class="texte">To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.</p>
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<p class="title2">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
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<p class="title1">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
<p class="texte">Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.</p>
<p class="texte">Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.</p>
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<p class="title2">Choice of the chassis <p/>
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<p class="title1">Choice of the chassis </p>
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<i>Bacillus subtilis</i> has been reported to be an endophytic bacterium of a large variety of plants and trees. This model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer Bacillus subtilis so as to fight Canker Stain from the inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.
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<p class="texte"><i>Bacillus subtilis</i> has been reported to be an endophytic bacterium of a large variety of plants and trees. This model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer Bacillus subtilis so as to fight Canker Stain from the inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.</p>
        
        

Revision as of 12:16, 10 October 2014