Team:Toulouse/Project/Overviews

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Among many other things, Southern France is well-known for the gorgeousness of its landscapes. Plane trees (Platanus sp.) are widely present and participate to the charm of this area, especially along the famous “Canal du Midi”. It is impossible to imagine this UNESCO World Heritage masterpiece without its trees. Unfortunately, these trees are threatened by a severe fungal infection called Canker Stain, and today the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.
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Among many other things, Southern France is well-known for the gorgeousness of its landscapes. Plane trees (<i>Platanus sp.</i>) are widely present and participate to the charm of this area, especially along the famous “Canal du Midi”. It is impossible to imagine this UNESCO World Heritage masterpiece without its trees. Unfortunately, these trees are threatened by a severe fungal infection called Canker Stain, and today the only treatment consists in a costly preventive tree-cutting and implies significant ecological troubles.
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Facing this emergency, the students from the iGEM Toulouse Team decided to be committed to the protection of their local heritage. Indeed, using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Thanks to different genetic modules, the engineered bacterium called SubtiTree is first able to head towards the pathogen, then to bind to its cell wall in order to finally deliver different fungicides to save the tree from its invaders. Taking into account the ecological and ethical matters, our team thinks about how to limit the spreading of the optimized microorganism through different strategies. Although our project was born from a local problematic, it is transposable to other tree vascular diseases.
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Facing this emergency, the students from the iGEM Toulouse Team, who enjoy chilling in this peaceful place, decided to be committed to the protection of their local heritage. Using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Using different genetic modules, we engineered a bacterium (SubtiTree) capable of heading towards the pathogen, binding to its cell wall and finally delivering different fungicides to save the tree from its invaders. Our team also began to elaborate different strategies to prevent any accidental spreading of the optimized microorganism, thus limiting the ecological and ethical footprints of SubtiTree. Although our project originated from a very local and specific tree disease, it could be transposable to other plant diseases.
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<p class="title1">Choice of the chassis </p>
<p class="title1">Choice of the chassis </p>
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<p class="texte"><i>Bacillus subtilis</i> has been reported to be an endophytic bacterium of a large variety of plants and trees. This model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer <i>Bacillus subtilis</i> so as to fight Canker Stain from the inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.</p>
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<p class="texte"><i>Bacillus subtilis</i> has been reported to be an endophyte bacterium of a large variety of plants and trees. Therefore, this model organism is a perfect chassis for our project.  Already used to treat plant diseases and fungal pathogens, we aim to engineer <i>Bacillus subtilis</i> to fight Canker Stain from the inside of the tree. Injected directly in the tree sap, our smart bacterium will act as a curative and preventive drug.</p>
<center><img style="width:700px; margin: -10px 0 55px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg"></center>
<center><img style="width:700px; margin: -10px 0 55px 130px;" ; src="http://parts.igem.org/wiki/images/2/2b/Overview_.jpg"></center>
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<p class="legend">Figure 1: Schema of our general strategy</p>
<p class="title1">Chemotaxis<a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="title1">Chemotaxis<a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="texte">First, the bacterium targets the pathogen thanks to a chemotaxis module which recognizes the soluble molecules released by the fungus' cell wall (N-acetylglucosamine).</p>
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<p class="texte">First, the bacterium targets the pathogen with a chemotaxis module which recognizes the soluble molecules released by the fungus' cell wall (N-acetylglucosamine).</p>
<p class="title1">Binding<a href="http://2014.igem.org/Team:Toulouse/Project/binding"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="title1">Binding<a href="http://2014.igem.org/Team:Toulouse/Project/binding"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="texte">Then, SubtiTree binds onto the pathogen by a chimeric protein anchored to the bacterium peptidoglycan and which can make a bridge between bacterial cell wall and fungal chitin, the main component of the pathogen's cell wall.</p>
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<p class="texte">Then, SubtiTree binds onto the pathogen using a chimeric protein anchored to the bacterium peptidoglycan and which can make a bridge between bacterial cell wall and fungal chitin, the main component of the pathogen's cell wall.</p>
<p class="title1">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
<p class="title1">Fungicides<a href="http://2014.igem.org/Team:Toulouse/Project/Fungicides"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;"> Show more</a></p>
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<p class="texte">To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.</p>
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<p class="texte">In the third step, our designed bacterium fights the pathogen by producing a powerful treatment of three different fungicides.</p>
<p class="title1">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
<p class="title1">Spreading <a href="http://2014.igem.org/Team:Toulouse/Project/Spreading"; style="font-size: 13px; cursor: pointer; color: #888; margin-left: 10px;">Show more</a></p>
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<p class="texte">Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.</p>
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<p class="texte">Our team worked on different aspects to control SubtiTree's spreading. The aim is to prevent horizontal transfers between different bacteria and to limit the growth and the survival of the engineered bacterium inside the tree during one season.</p>
        
        

Latest revision as of 19:57, 17 October 2014