Team:Toulouse/Project/Overviews

From 2014.igem.org

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    <img style="width:700px" ; src="https://static.igem.org/mediawiki/2014/2/2b/Overview_.jpg">
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         <p class="texte">Fungal diseases lead to major economic losses all over the world.
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        Some pathogens trigger trachemycosis by disturbing the vascular system of the plant.
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        Canker is one of these infections especially affecting plane trees (Platanus sp.). Plane trees are widely present in            Southern France and particularly along the famous Canal du Midi, participating to the site gorgeousness.  
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        Today, the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.  
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Among many other things, Southern France is well-known for the gorgeousness of its landscapes. Plane trees (Platanus sp.) are widely present and participate to the charm of this area, especially along the famous “Canal du Midi”. It is impossible to imagine this UNESCO World Heritage masterpiece without its trees. Unfortunately, these trees are threatened by a severe fungal infection called Canker, and today the only treatment consists in preventive tree-cutting which has a huge cost and implies significant ecological troubles.
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        Facing this emergency, our team offers an innovative alternative originated from synthetic biology.</p>
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Facing this emergency, the students from the iGEM Toulouse Team decided to be committed to the protection of their local heritage. Indeed, using a bacterium vector naturally present in the trees, our team offers an alternative solution originated from synthetic biology. Thanks to different genetic modules, the engineered bacterium called SubtiTree is first able to head towards the pathogen, then to bind to its cell wall in order to finally deliver different fungicides to save the tree from its invaders. Taking into account the ecological and ethical matters, our team thinks about how to limit the spreading of the optimized microorganism through different strategies. Although our project was born from a local problematic, it is transposable to other tree vascular diseases.<br>
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<img style="width:700px" ; src="https://static.igem.org/mediawiki/2014/2/2b/Overview_.jpg">
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<br><br>Chemotaxis Show more </br>
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First, the bacterium targets the pathogen thanks to a chemotaxis module which recognizes the soluble chitin monomers (N-acetyl-glucosamine) released by the fungi.
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<br><br>Fixation Show more
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<br>Then, SubtiTree binds onto the pathogen by a chimeric protein anchored on one side to the bacterium peptidoglycan and on the other side to the fungal chitin-based wall.
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<br><br>Fungicides Show more
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<br>To finish, our designed bacterium fights against the pathogen by setting up a powerful treatment based on the production of three different fungicides.
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<br><br>Spreading Show more
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<br>Our team worked on different aspects to keep control on SubtiTree. The aim is to prevent horizontal transfers between different bacteria and to contain the engineered bacterium inside the tree during one season.
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</p>
    
    
      
      
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       <P class="texte">The aim of our project is to make an endophytic bacterium capable of detecting the pathogen, and targeting it in order  to deliver different fungicides to save the tree from its invaders.
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       <P class="texte"></P>  
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      Considering ecological and ethical matters, this project limits the spreading of our engineered bacterium in the environment.</P>  
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Revision as of 13:02, 7 October 2014