Team:Toulouse/Project/Fungicides

From 2014.igem.org

(Difference between revisions)
Line 94: Line 94:
<p class="legend">Figure 1: Schema of the fungicide module</p></center>
<p class="legend">Figure 1: Schema of the fungicide module</p></center>
-
         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. This triple therapy minimizes the risk of resistance.</p> <br>
+
         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree.
 +
In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. This triple therapy provides
 +
the advantage to minimize the resistance phenomenon.</p> <br>
-
  <p class="textesimple">Originally from plants, these peptides have different targets to maximize the lethality on <i>C. platani</i>.</p>
+
  <p class="textesimple">Originally from plants, these peptides have different targets thus increasing the lethality on <i>C. platani</i>.</p>
<br></br>  
<br></br>  
<ul>
<ul>
-
<li class="tree"><p class="texte"><b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (AntiMicrobial Peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi.</p></li>
+
<li class="tree"><p class="texte"><b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (AntiMicrobial Peptides) made of 17 amino acids
 +
which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi.</p></li>
-
<li class="tree"><p class="texte"><b>GAFP-1 </b>(<i>Gastrodia</i> Anti Fungal Protein 1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid <i>Gastrodia elata</i>, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 accumulates in nutritive corms where the fungal infection takes place, and <i>in vitro</i> assays demonstrated it can inhibit the growth of ascomycete and basidiomycete fungal plant pathogens.</p></li>
+
<li class="tree"><p class="texte"><b>GAFP-1 </b>(<i>Gastrodia</i> Anti Fungal Protein 1), also known as gastrodianin, is a mannose and chitin binding lectin
 +
originating from the Asiatic orchid <i>Gastrodia elata</i>, a traditional Chinese medicinal herb cultured for thousands of years.  
 +
GAFP-1 accumulates in nutritive corms where the fungal infection takes place, and <i>in vitro</i> assays demonstrated it can inhibit the growth of  
 +
ascomycete and basidiomycete fungal plant pathogens.</p></li>
-
<li class="tree"><p class="texte"><b>EcAMP-1 </b>(<i>Echinochloa crus-galli</i> AntiMicrobial Peptide) consists in 37 amino acids inhibiting hyphae elongation. EcAMP-1 is the first example of AMP with a novel disulfide-stabilized-α helical hairpin fold. It is isolated from kernels of barnyard grass. EcAMP-1 exhibits high activity against fungi of the genus <i>Fusarium</i>.</p></li>
+
<li class="tree"><p class="texte"><b>EcAMP-1 </b>(<i>Echinochloa crus-galli</i> AntiMicrobial Peptide) consists in 37 amino acids inhibiting hyphae elongation
 +
EcAMP-1 is the first example of AMP with a novel disulfide-stabilized-α helical hairpin fold. It is isolated from kernels of barnyard grass.
 +
EcAMP-1 exhibits high activity against fungi of the genus <i>Fusarium</i>.</p></li>
</ul>
</ul>
</p>
</p>
Line 109: Line 117:
<p class="title1" style="margin-top:30px;">More information about this module </p>
<p class="title1" style="margin-top:30px;">More information about this module </p>
<p  class="texte">
<p  class="texte">
-
We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the three genes coding for the antifungal peptides are placed under the control of the constitutive promoter P<sub>veg</sub> in <i>Bacillus subtilis</i>.</p>
+
We built different genetic constructions to test each fungicide separately and to test them all together on the same operon. The three genes coding for the
 +
antifungal peptides are placed under the control of the constitutive promoter P<sub>veg</sub> in <i>Bacillus subtilis</i>.</p>
<center><img style="width:930px; float:left; margin: 30px 0 45px;" src="http://parts.igem.org/wiki/images/d/d0/Fungicideprod.jpg">
<center><img style="width:930px; float:left; margin: 30px 0 45px;" src="http://parts.igem.org/wiki/images/d/d0/Fungicideprod.jpg">
Line 116: Line 125:
<p class="title2">Added parts</p>
<p class="title2">Added parts</p>
<p class="title3">EcAMP-1</p>
<p class="title3">EcAMP-1</p>
-
<p  class="texte">EcAMP-1 was already present in the Registry, added by the Utah State 2013 iGEM team (<a href="http://parts.igem.org/Part:BBa_K1162001"_blank">BBa_K1162001</a>). This part has been modified and improved by our team (<a href="http://parts.igem.org/Part:BBa_K1364019"_blank">BBa_K1364019</a>).  </p>
+
<p  class="texte">EcAMP-1 was already present in the Registry, added by the Utah State 2013 iGEM team  
 +
(<a href="http://parts.igem.org/Part:BBa_K1162001"_blank">BBa_K1162001</a>). This part has been modified and improved by our team  
 +
(<a href="http://parts.igem.org/Part:BBa_K1364019"_blank">BBa_K1364019</a>) with the addition of a STOP codon after the coding sequence.  </p>
<p class="title3">D4E1 and GAFP-1</p>
<p class="title3">D4E1 and GAFP-1</p>
<p class="texte">We added D4E1 and GAFP-1 to the Registry of Standard Biological Parts (See <a href="http://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"_blank">Submitted parts</a>). <br>We ordered the genes to a synthesis company and did cloning. These new BioBricks were designed in order to be expressed and secreted with <i>Bacillus subtilis</i>.  </p>
<p class="texte">We added D4E1 and GAFP-1 to the Registry of Standard Biological Parts (See <a href="http://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts"_blank">Submitted parts</a>). <br>We ordered the genes to a synthesis company and did cloning. These new BioBricks were designed in order to be expressed and secreted with <i>Bacillus subtilis</i>.  </p>
<br>
<br>
<p  class="title2">Secretion</p>
<p  class="title2">Secretion</p>
-
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequences of D4E1 and GAFP-1 were flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.</p><br>
+
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequences of D4E1 and GAFP-1 were flanked on the N-terminal end with
 +
a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.</p><br>

Revision as of 20:41, 17 October 2014