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Team:Toulouse/Project/Fungicides
From 2014.igem.org
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<p> Originated from plants, these peptides have different target to maximize the lethality on C. platani. | <p> Originated from plants, these peptides have different target to maximize the lethality on C. platani. | ||
<p> | <p> | ||
| - | - < | + | - <b>D4E1</b> is a synthetic peptide made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p> |
<p> | <p> | ||
| - | - GAFP-1 (Gastrodia Anti Fungal Protein 1) is a lectin binding to mannose thus inhibiting the growth of a variety of fungi. </p> | + | - <b>GAFP-1 </b>(Gastrodia Anti Fungal Protein 1) is a lectin binding to mannose thus inhibiting the growth of a variety of fungi. </p> |
<p> | <p> | ||
| - | - EcAMP-1 (Echinochloa crus-galli Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation. </p> | + | - <b>EcAMP-1 </b>(Echinochloa crus-galli Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation. </p> |
</p> | </p> | ||
| - | <p> | + | <p>More information on this module : </p> |
| - | + | <p>EcAMP-1 was already present in the Registry, added by the Utah State 2013 iGEM team (BB_K1162001). We added D4E1 and GAFP-1 to the Registry of Standard Biological Parts (see parts). These new BioBricks were designed in order to be expressed and secreted with Bacillus subtilis. | |
| - | <p> | + | </p> |
| + | <p> </p> | ||
| + | <p>Secretion : </p> | ||
| + | <p>The coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process. </p> | ||
| + | .</P> | ||
Revision as of 12:21, 1 October 2014
Fungicides
To eradicate fungal diseases
Project > Fungicides
The main objective of SubtiTree is to ensure the destruction of the pathogenic fungi inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities.
Originated from plants, these peptides have different target to maximize the lethality on C. platani.
- D4E1 is a synthetic peptide made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi.
- GAFP-1 (Gastrodia Anti Fungal Protein 1) is a lectin binding to mannose thus inhibiting the growth of a variety of fungi.
- EcAMP-1 (Echinochloa crus-galli Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation.
More information on this module :
EcAMP-1 was already present in the Registry, added by the Utah State 2013 iGEM team (BB_K1162001). We added D4E1 and GAFP-1 to the Registry of Standard Biological Parts (see parts). These new BioBricks were designed in order to be expressed and secreted with Bacillus subtilis.
Secretion :
The coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.
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