Team:Toulouse/Project/Fungicides

From 2014.igem.org

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<p class="legend">Figure n°1: Scheme of the fungicide module</p></center>
         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. </p> <br>
         <p class="textesimple">The main objective of SubtiTree is to ensure the <b> destruction of the pathogenic fungi </b> inside the tree. In order to achieve this goal, we built a genetic module to produce three different peptides with antifungal activities. </p> <br>
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<li class="tree"><p class="texte"><b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (antimicrobial peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p></li>
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<li class="tree"><p class="texte"><b>D4E1</b> is a synthetic peptide analog to Cecropin B AMPs (AntiMicrobial Peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p></li>
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<li class="tree"><p class="texte"><b>GAFP-1 </b>(<i>Gastrodia</i> Anti Fungal Protein 1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP1 accumulates in nutritive corms where the fungal infection takes place, and <i>in vitro</i> assays demonstrated it can inhibit the growth of ascomycete and basidiomycete fungal plant pathogens.</p></li>
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<li class="tree"><p class="texte"><b>GAFP-1 </b>(<i>Gastrodia</i> Anti Fungal Protein 1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP-1 accumulates in nutritive corms where the fungal infection takes place, and <i>in vitro</i> assays demonstrated it can inhibit the growth of ascomycete and basidiomycete fungal plant pathogens.</p></li>
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<li class="tree"><p class="texte"><b>EcAMP-1 </b>(<i>Echinochloa crus-galli </i> Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation. EcAMP1 is the first example of AMP with a novel disulfide-stabilized-α helical hairpin fold. It is isolated from kernels of barnyard grass. EcAMP1 exhibits high activity against fungi of the genus Fusarium.</p></li>
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<li class="tree"><p class="texte"><b>EcAMP-1 </b>(<i>Echinochloa crus-galli </i> AntiMicrobial Peptide) consists in 37 amino acids inhibiting hyphae elongation. EcAMP-1 is the first example of AMP with a novel disulfide-stabilized-α helical hairpin fold. It is isolated from kernels of barnyard grass. EcAMP-1 exhibits high activity against fungi of the genus <i>Fusarium</i>.</p></li>
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<p class="title1" style="margin-top:30px;">More information on this module </p>
<p class="title1" style="margin-top:30px;">More information on this module </p>
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We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the 3 genes coding for the antifungal peptides are placed under the control of a constitutive promoter in <i>Bacillus subtilis</i>: Pveg. </p>
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We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the three genes coding for the antifungal peptides are placed under the control of the constitutive promoter P<sub>veg</sub> in <i>Bacillus subtilis</i>.</p>
<img style="width:930px; float:left; margin: 30px 0 45px;" src="http://parts.igem.org/wiki/images/d/d0/Fungicideprod.jpg">  
<img style="width:930px; float:left; margin: 30px 0 45px;" src="http://parts.igem.org/wiki/images/d/d0/Fungicideprod.jpg">  

Revision as of 13:53, 16 October 2014