Team:Toulouse/Project/Fungicides

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<p class="title2" style="margin-top:30px;"><b>More information on this module : </p></b> <br>
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<p class="title1" style="margin-top:30px;"><b>More information on this module </p></b> <br>
<p  class="texte">Final constructions: (see Parts to have more details on the intermediate parts)
<p  class="texte">Final constructions: (see Parts to have more details on the intermediate parts)
We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the 3 genes are placed under the control of a constitutive promoter in Bacillus subtilis: Pveg. </p>
We built different genetic constructions to test each fungicide separately and to test them all together on the same operon where the 3 genes are placed under the control of a constitutive promoter in Bacillus subtilis: Pveg. </p>
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<p  class="title2"><b>Secretion :</b> </p>
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<p  class="title1"><b>Secretion </b> </p>
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.  </p> <br>
<p  class="texte">In order to export the peptides outside the bacteria, the coding sequence of D4E1 and GAFP-1 was flanked on the N-terminal end with a signal peptide (amyE signal peptide) followed by a pro peptide, cleaved during the secretion process.  </p> <br>

Revision as of 12:30, 10 October 2014