Team:Toulouse/Project/Fungicides

From 2014.igem.org

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  <p class="textesimple"> Originated from plants, these peptides have different target to maximize the lethality on C. platani.  
  <p class="textesimple"> Originated from plants, these peptides have different target to maximize the lethality on C. platani.  
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- <b>D4E1</b> is a synthetic peptide made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p>
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- <b>D4E1</b> is a synthetic peptide anlogo to Cecropin B AMPs (antimicrobial peptides) made of 17 amino acids which has been shown to have an antifungal activity by complexing with a sterol present in the conidia’s wall of numerous fungi. </p>
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- <b>GAFP-1 </b>(Gastrodia Anti Fungal Protein 1) is a lectin binding to mannose thus inhibiting the growth of a variety of fungi. </p>
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- <b>GAFP-1 </b>(<i>Gastrodia</i> Anti Fungal Protein 1), also known as gastrodianin, is a mannose and chitin binding lectin originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years. GAFP1 accumulates in nutritive corms where the fungal infection takes place, and in vitro assays demonstrated it can inhibit the growth of ascomycete and basidiomycete fungal plant pathogens.</p>
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- <b>EcAMP-1 </b>(Echinochloa crus-galli Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation. </p>
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- <b>EcAMP-1 </b>(<i>Echinochloa crus-galli </i> Anti Microbial Peptide) consists in 37 amino acids inhibiting hyphae elongation. EcAMP1 is the first example of AMP with a novel disulfide-stabilized-α helical hairpin fold. It is isolated from kernels of barnyard grass. EcAMP1 exhibits high activity against fungi of the genus Fusarium.</p>
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<p  class="title1"><b>References</b> </p>
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<p  class="texte">
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- A.J De Lucca, J.M Bland, C. Grimm, T.J Jacks.<b> Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1 </b>. Canadian Journal of Microbiology. 1998, Vol. 44:514-520. <br>
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Kanniah Rajasekaran, Kurt D. Stromberg, Jeffrey W. Cary, and Thomas E. Cleveland.<b> Broad-Spectrum Antimicrobial Activity in vitro of the Synthetic Peptide D4E1</b>. J. Agric. Food Chem. 2001, Vol. 49, 2799-2803.<br>
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-M. Visser, D. Stephan, J.M. Jaynes and J.T. Burger.<b> A transient expression assay for the in planta efficacy screening of an antimicrobial peptide against grapevine bacterial pathogens</b>. Letters in Applied Microbiology. 2012, Vol. 54, 543–551.<br>
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-K. D. Cox, D. R. Layne, R. Scorza, G Schnabel. <b>Gastrodia anti-fungal protein from the orchid Gastrodia elata confers disease resistance to root pathogens in transgenic tobacco</b>. Planta. 2006, Vol. 224:1373–1383<br>
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-Xiaochen Wang, Guy Bauw, Els J.M. Van Damme, Willy J. Peumans, Zhang-Liang Chen, Marc Van Montagu and Willy Dillen. <b>Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties</b>. The Plant Journal. 2001, Vol. 25(6), 651±661<br>
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-Svetlana B. Nolde, Alexander A. Vassilevski, Eugene A. Rogozhin, Nikolay A. Barinov, Tamara A. Balashova, Olga V. Samsonova, Yuri V. Baranov, Alexey S. Arseniev and Eugene V. Grishin. <b>Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli)</b>. The journal of Biological Chemistry. 2011, Vol. 286, 25145–25153<br>
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Revision as of 09:19, 11 October 2014