Team:Toulouse/Project/Fixation

From 2014.igem.org

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SubtiTree binds to the fungal pathogen





First, the attachment of the bacterium is allowed by the introduction of a chimeric protein recognizing bacterial peptidoglycan and carrying a binding domain chitin - CBD - which, in contrast to the receiver of chemotaxis, would recognize a long oligomer. We make a fusion protein domain CWB LytC B. subtilis and domain 4 GBPA V. cholerae. The verification of the construction is made thanks to an ELISA test.