Team:Toulouse/Project/Fixation
From 2014.igem.org
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- | First, the attachment of the | + | First, the attachment of the bacterium is allowed by the introduction of a chimeric protein recognizing bacterial peptidoglycan and carrying a binding domain chitin - CBD - which, in contrast to the receiver of chemotaxis, would recognize a long oligomer. We make a fusion protein domain CWB LytC B. subtilis and domain 4 GBPA V. cholerae. The verification of the construction is made thanks to an ELISA test.</font></div></td> |
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<p><img style="width:400px" ; src="https://static.igem.org/mediawiki/2014/9/90/Champignon_petidoglycan_binding.JPG"></p> | <p><img style="width:400px" ; src="https://static.igem.org/mediawiki/2014/9/90/Champignon_petidoglycan_binding.JPG"></p> |
Latest revision as of 11:55, 12 September 2014
First, the attachment of the bacterium is allowed by the introduction of a chimeric protein recognizing bacterial peptidoglycan and carrying a binding domain chitin - CBD - which, in contrast to the receiver of chemotaxis, would recognize a long oligomer. We make a fusion protein domain CWB LytC B. subtilis and domain 4 GBPA V. cholerae. The verification of the construction is made thanks to an ELISA test. |