Team:Toulouse/Project/Chemotaxis

(Difference between revisions)
 Revision as of 12:23, 15 October 2014 (view source)Kanitzer (Talk | contribs)← Older edit Revision as of 12:24, 15 October 2014 (view source)Kanitzer (Talk | contribs) Newer edit → Line 101: Line 101: Some bacteria are attracted by NAG, like Vibrio cholerae which has a N-acetylglucosamine regulated methyl-accepting chemotaxis protein, henceforth called with the simple name of VCD.

Some bacteria are attracted by NAG, like Vibrio cholerae which has a N-acetylglucosamine regulated methyl-accepting chemotaxis protein, henceforth called with the simple name of VCD.

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Therefore, our idea is to switch the natural glucose specificity of B. subtilis by a NAG specificity. To achieve this, we need to change the extracellular domain of McpA, the domain responsible for the specificity, by the extracellular domain of VCD. Therefore, our idea is to switch the natural glucose specificity of B. subtilis by a NAG specificity. To achieve this, we need to change the extracellular domain of McpA, the domain responsible for the specificity, by the extracellular domain of VCD. The whole sequence has been designed in silico and codon optimized for the transcription in Bacillus subtilis before its synthesis.

The whole sequence has been designed in silico and codon optimized for the transcription in Bacillus subtilis before its synthesis.

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References

References