Team:Toulouse/Project/Chemotaxis

From 2014.igem.org

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<p class="title1">More information on this module</p>
<p class="title1">More information on this module</p>
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Chemotaxis is used as a way to detect and approach fungi. Indeed during its growth, fungi release N-acetylglucosamine (NAG), the basic unit of chitin which composed its cell wall. Thus, the concentration of N-acetylglucosamine is getting more important around fungi.</p>
Chemotaxis is used as a way to detect and approach fungi. Indeed during its growth, fungi release N-acetylglucosamine (NAG), the basic unit of chitin which composed its cell wall. Thus, the concentration of N-acetylglucosamine is getting more important around fungi.</p>
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  Some bacteria are attracted by NAG, like <i>Vibrio cholerae</i> which has a N-acetylglucosamine regulated methyl-accepting chemotaxis protein, henceforth called with the simple name of<b> VCD</b>.</p>
  Some bacteria are attracted by NAG, like <i>Vibrio cholerae</i> which has a N-acetylglucosamine regulated methyl-accepting chemotaxis protein, henceforth called with the simple name of<b> VCD</b>.</p>
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<center><img width="500px" SRC="http://2014.igem.org/wiki/images/c/cf/Schema_chemotaxis.png" alt="schema"></center>
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<center><img width="500px" SRC="http://2014.igem.org/wiki/images/c/cf/Schema_chemotaxis.png" alt="schema" style="margin-bottom:20px;"></center>
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Therefore, our idea is to switch the natural glucose specificity of <i>B. subtilis</i> by a NAG specificity. To achieve this, we need to change the extracellular domain of McpA, the domain responsible for the specificity, by the extracellular domain of VCD.
Therefore, our idea is to switch the natural glucose specificity of <i>B. subtilis</i> by a NAG specificity. To achieve this, we need to change the extracellular domain of McpA, the domain responsible for the specificity, by the extracellular domain of VCD.
The whole sequence has been designed <i>in silico</i> and codon optimized for the transcription in <i>Bacillus subtilis</i> before its synthesis.</p>
The whole sequence has been designed <i>in silico</i> and codon optimized for the transcription in <i>Bacillus subtilis</i> before its synthesis.</p>
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<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Gene_chemotaxis.png" alt="gene construct"></center>
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<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Gene_chemotaxis.png" alt="gene construct" style="margin-bottom:20px;"></center>
<p class="title1">References</p>
<p class="title1">References</p>

Revision as of 12:23, 15 October 2014