Team:Toulouse/Project/Chemotaxis

From 2014.igem.org

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<p class="title2">What is chemotaxis?</p>
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<b>WHAT IS CHEMOTAXIS ?</b>
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Chemotaxis is a bacterial function which allows bacteria to move according a concentration gradient. With this system bacteria can find better place to grow by swimming toward higher concentrations of molecules, such as nutritional molecules like sugar, amino acid, vitamins...
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Chemotaxis is a bacterial function which allows to bacteria to move according a concentration gradient. This system allows bacteria to find better place to growth, the chemotaxis system is very used by bacteria especially for nutritional molecules like sugar, amino acid, vitamins ...
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In our case, chemotaxis is used as a way to detect and approach fungus. Indeed during its growth, fungi release N-acetylglucosamine, a monomer of chitin which is specific to fungal presence. Thus, there is a gradient of N- acetylglucosamine around fungi.</p>
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In our case chemotaxis is used as a way to detect and approach fungus. Indeed during its growth, fungi release N-acetylglucosamine, this molecule is the monomer of chitin which is specific to fungal presence. So around fungi there is a gradient of N- acetylglucosamine.</p>
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<center><img style="width:420px; " src="http://parts.igem.org/wiki/images/e/e9/Recap_chemotax.jpg"></center>
<center><img style="width:420px; " src="http://parts.igem.org/wiki/images/e/e9/Recap_chemotax.jpg"></center>
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Unfortunately bacillus Subtilis isn’t able to detect such gradient, but bacillus is able to detect and move to sugar like glucose thanks a glucose specific receptor, VcpA. But other bacteria can detect these kind of molecule, for example vibrio cholera, which have a specific receptor for N- acetylglucosamine, VCD.</p>
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Unfortunately <i>Bacillus subtilis</i> is not able to detect such gradient, but <i>Bacillus</i> is able to detect and to move towards sugar like glucose thanks a glucose specific receptor, VcpA. But other bacteria can detect these kind of molecule, for example <i>Vibrio cholerae</i>, which has a specific receptor for N- acetylglucosamine, VCD.</p>
<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Schema_chemotaxis.png" alt="schema"></center>
<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Schema_chemotaxis.png" alt="schema"></center>
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Therefore, the idea is pretty simple, we switch glucose specificity by N- acetylglucosamine specificity. So we need to modify the extracellular part of VcpA, the part which is responsible of the specificity, by the extracellular part of a N-acetylglucosamine receptor, VCD.</p>
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Therefore, our idea is pretty simple, we switch glucose specificity by N-acetylglucosamine specificity. So we need to modify the extracellular part of VcpA, the part which is responsible of the specificity, by the extracellular part of a N-acetylglucosamine receptor, VCD.</p>
<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Gene_chemotaxis.png" alt="gene construct"></center>
<center><img SRC="http://2014.igem.org/wiki/images/c/cf/Gene_chemotaxis.png" alt="gene construct"></center>

Revision as of 18:28, 13 October 2014