Team:Toulouse/Project/Chemotaxis

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Chemotaxis is used as a way to detect and come close to the location of fungi infection. During its growth, fungi release N-acetylglucosamine (NAG), the basic unit of chitin which composed its cell wall. Thus, there should exist a gradient of the concentration of NAG around the fungi.</p>
Chemotaxis is used as a way to detect and come close to the location of fungi infection. During its growth, fungi release N-acetylglucosamine (NAG), the basic unit of chitin which composed its cell wall. Thus, there should exist a gradient of the concentration of NAG around the fungi.</p>
<p class="texte">
<p class="texte">
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It is known that <i>B. subtilis</i> is able to detect and to swim towards glucose using the Methyl-accepting chemotaxis protein, henceforth called <b>McpA</b>(<a> href="MCPA_BACSU"_blanck">MCPA_BACSU).<br>
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It is known that <i>B. subtilis</i> is able to detect and to swim towards glucose using the Methyl-accepting chemotaxis protein, henceforth called <b>McpA</b> (<a href="http://www.uniprot.org/uniprot/P39214"_blanck">MCPA_BACSU</a>).<br>
Some bacteria are attracted by NAG, like <i>Vibrio cholerae</i> which has a NAG regulated methyl-accepting chemotaxis protein: <b>VCD</b> (<a href="http://www.uniprot.org/uniprot/C3NYT2"_blank">VCD_000306</a>).</p>
Some bacteria are attracted by NAG, like <i>Vibrio cholerae</i> which has a NAG regulated methyl-accepting chemotaxis protein: <b>VCD</b> (<a href="http://www.uniprot.org/uniprot/C3NYT2"_blank">VCD_000306</a>).</p>
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<center><img width="600px" SRC="https://static.igem.org/mediawiki/2014/e/e4/Chimio2.png" alt="gene construct" style="margin-bottom:40px;"></center>
<center><img width="600px" SRC="https://static.igem.org/mediawiki/2014/e/e4/Chimio2.png" alt="gene construct" style="margin-bottom:40px;"></center>
<p class="legend">Figure 3: Construction of the chemotaxis gene</p>
<p class="legend">Figure 3: Construction of the chemotaxis gene</p>
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<center><a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results"> <img src="https://static.igem.org/mediawiki/parts/f/fe/Jump.jpg"> </a></center>
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<p class="title1">References</p>
<p class="title1">References</p>
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<ul>
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<li class="tree"><p class="texte">K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman and G. Schoolnik.<b> The Vibrio cholerae chitin utilization program </b>. The National Academy of Sciences of the USA (2004).</p></li>
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<li class="tree"><p class="texte">K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman, and G. Schoolnik.<b> The <i>Vibrio cholerae</i> chitin utilization program </b>. The National Academy of Sciences of the USA (2004).</p></li>
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<li class="tree"><p class="texte">C. Kristich and GW. Ordal. <b><i>Bacillus subtilis</i> CheD is a chemoreceptor modification enzyme required for chemotaxis</b>. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.<br></p></li>
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<li class="tree"><p class="texte">C. Kristich, and GW. Ordal. <b><i>Bacillus subtilis</i> CheD is a chemoreceptor modification enzyme required for chemotaxis</b>. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.<br></p></li>
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Latest revision as of 02:59, 18 October 2014