Team:Toulouse/Project/Chemotaxis

From 2014.igem.org

(Difference between revisions)
 
(31 intermediate revisions not shown)
Line 15: Line 15:
  <style type="text/css">
  <style type="text/css">
-
   .title1{color:#20a8da; font-family:'Open Sans'; font-weight:600; font-size:24px;  margin:0 0 33px 0; border:none;}
+
   .title1{color:green; font-family:'Open Sans'; font-weight:600; font-size:24px;  margin:0 0 33px 0; border:none;}
   .title2{color:#5a6060; font-family:'Open Sans'; font-weight:600; font-size:18px; margin:0 0 30px 0; border:none;}
   .title2{color:#5a6060; font-family:'Open Sans'; font-weight:600; font-size:18px; margin:0 0 30px 0; border:none;}
Line 36: Line 36:
.banniere-content{
.banniere-content{
-
  background-color: rgba(46,204,113, 0.6);
+
  background-color: rgba(130,196,108, 0.7);
padding:28px 28px 0;
padding:28px 28px 0;
position:absolute;
position:absolute;
Line 45: Line 45:
color:white
color:white
}
}
 +
 +
p.legend{color:#5a6060; font-family:'Open Sans'; font-size:14px; margin:0 0 50px 0; line-height:24px; text-align: center;}
.banniere-content h2{
.banniere-content h2{
Line 55: Line 57:
font-size:16px
font-size:16px
}
}
 +
 +
li.tree {
 +
  display : list-item;
 +
list-style-image: url(https://static.igem.org/mediawiki/2014/a/a7/388438arbrefleurs.png);
 +
}
</style>
</style>
Line 65: Line 72:
     <div class="banniere-content">
     <div class="banniere-content">
       <h2>Chemotaxis</h2>
       <h2>Chemotaxis</h2>
-
       <p>To target the pathogenic fungus.</p>
+
       <p>To target the pathogenic fungus</p>
     </div>
     </div>
   </div>
   </div>
Line 76: Line 83:
   <div id="innercontenthome">
   <div id="innercontenthome">
-
       <div class="centering" style="padding-top: 85px; padding-bottom:40px;">
+
       <div class="centering" style="padding-top: 65px; padding-bottom:40px;">
   <!--Short description : à changer!!!-->
   <!--Short description : à changer!!!-->
-
        <p class="texte">Fusce et fermentum nibh. Aliquam ornare venenatis odio vel interdum. Ut egestas elementum dolor, sit amet interdum metus gravida id. Morbi dictum commodo ipsum, eu maximus lectus pretium vel. Vivamus sit amet sem orci. Nulla leo ante, interdum id eros nec, rutrum suscipit sapien. Aenean posuere mauris a nisl laoreet congue. Sed aliquam vulputate risus, ut bibendum ligula consequat vel. Proin rhoncus orci eu massa dapibus elementum. Duis eu lacinia velit. Ut lacinia eu sem suscipit euismod. Proin sollicitudin nibh ut dapibus vulputate.</p>
 
-
<p class="texte">Quisque nec tempus mi. Sed sit amet justo tellus. Vestibulum pulvinar viverra felis sed aliquet. Suspendisse sed turpis eu justo finibus dictum quis vel nisi. Mauris quis imperdiet sem. Aenean scelerisque commodo orci a varius. Integer turpis odio, elementum a venenatis vitae, ultricies quis quam. Phasellus cursus ligula vel ex rutrum placerat. Curabitur dictum nisi risus, eu finibus ex fringilla vitae. Aenean consequat, ante elementum viverra laoreet, arcu nulla aliquam velit, pretium imperdiet neque felis a diam. Suspendisse ultricies nisi non ex finibus pharetra ac et ipsum. Nulla vulputate dolor urna, et semper nisl tempor vitae. Sed cursus dolor ut consequat posuere.</p>
 
-
<p class="texte">Ut at massa quam. Vestibulum rutrum dapibus tortor ut facilisis. Pellentesque condimentum quam id ante varius imperdiet. Quisque mauris urna, finibus in mauris vel, laoreet volutpat libero. Aliquam erat volutpat. Vivamus non imperdiet nibh. Sed fringilla tristique metus, non fermentum quam tincidunt non. Praesent dictum faucibus massa, eu bibendum sapien iaculis in. Ut porta tortor eu elementum interdum. Vivamus non tempor ligula. In accumsan ligula non ligula aliquam, at dictum dolor cursus. Ut iaculis ultrices ex et faucibus. Praesent bibendum sagittis elit, eget ultricies dolor imperdiet id.</P>  
+
<center><img style="width:420px; " src="https://static.igem.org/mediawiki/parts/e/e9/Recap_chemotax.jpg"></center>
 +
<p class="legend">Figure 1: Schema of the chemotaxis module</p>
 +
 
 +
<p class="title1">What is chemotaxis?</p>
 +
 
 +
<p class="texte">
 +
Chemotaxis is a bacterial function which induces a movement toward a gradient of concentration of a molecule of interest. With this system the bacteria are able to swim to a location containing higher concentrations of molecules such as sugar, amino acid, vitamins...
 +
Chemotactic-signal transducers respond to changes in the concentration of attractants and repellents in the environment, transduce the signal from the outside to the inside of the cell, and facilitate sensory adaptation through the variation of the level of methylation.  
 +
 
 +
<p class="title1">More information on this module</p>
 +
<p class="texte">
 +
Chemotaxis is used as a way to detect and come close to the location of fungi infection. During its growth, fungi release N-acetylglucosamine (NAG), the basic unit of chitin which composed its cell wall. Thus, there should exist a gradient of the concentration of NAG around the fungi.</p>
 +
<p class="texte">
 +
It is known that <i>B. subtilis</i> is able to detect and to swim towards glucose using the Methyl-accepting chemotaxis protein, henceforth called <b>McpA</b> (<a href="http://www.uniprot.org/uniprot/P39214"_blanck">MCPA_BACSU</a>).<br>
 +
Some bacteria are attracted by NAG, like <i>Vibrio cholerae</i> which has a NAG regulated methyl-accepting chemotaxis protein: <b>VCD</b> (<a href="http://www.uniprot.org/uniprot/C3NYT2"_blank">VCD_000306</a>).</p>
 +
 
 +
<center><img width="500px" SRC="https://static.igem.org/mediawiki/2014/4/47/Chimio1.png" alt="schema" style="margin-bottom:60px;"></center>
 +
<p class="legend">Figure 2: Chimeric protein of chemotaxis</p>
 +
 
 +
<p class="texte">
 +
Therefore, our idea is to switch the natural glucose specificity of <i>B. subtilis'</i>, mediated by McpA, to a NAG specificity. To achieve this, we need to change the extracellular domain of McpA, responsible for the specificity, by the extracellular domain of VCD.
 +
The whole sequence has been designed <i>in silico</i> and codon optimized for the transcription in <i>B. subtilis</i> before its synthesis.</p>
 +
 
 +
<center><img width="600px" SRC="https://static.igem.org/mediawiki/2014/e/e4/Chimio2.png" alt="gene construct" style="margin-bottom:40px;"></center>
 +
<p class="legend">Figure 3: Construction of the chemotaxis gene</p>
 +
 
 +
<center><a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results"> <img src="https://static.igem.org/mediawiki/parts/f/fe/Jump.jpg"> </a></center>
 +
 
 +
 
 +
<p class="title1">References</p>
 +
<ul>
 +
<li class="tree"><p class="texte">K. Meibom,L. Xibing, A. Nielsen, CY. Wu, S. Roseman, and G. Schoolnik.<b> The <i>Vibrio cholerae</i> chitin utilization program </b>. The National Academy of Sciences of the USA (2004).</p></li>
 +
<li class="tree"><p class="texte">C. Kristich, and GW. Ordal. <b><i>Bacillus subtilis</i> CheD is a chemoreceptor modification enzyme required for chemotaxis</b>. J Biol Chem. 2002 Jul 12;277(28):25356-62. Epub 2002 May 13.<br></p></li>
 +
</ul>
      
      
   <!-- Navigation section -->   
   <!-- Navigation section -->   
Line 91: Line 129:
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
   <div class="page-nav" style="border-top:1px solid #cccccc; padding-top:40px; margin-top:40px;">
-
     <a href="https://2014.igem.org/Team:Toulouse/Project/binding" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Binding
+
     <a href="https://2014.igem.org/Team:Toulouse/Project/Overviews" class="page-nav-right" style="width:447px; float:left; display:block;text-decoration:none; color:#666; font-size:18px;">Overview
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
       <img src="https://static.igem.org/mediawiki/2014/2/26/Template-igem2014-img-arrowleft.png" style="display:block; padding-top:10px;"/>
     </a>  
     </a>  
Line 99: Line 137:
     </div>
     </div>
-
     <a href="https://2014.igem.org/Team:Toulouse/Project/Fungicides" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
+
     <a href="https://2014.igem.org/Team:Toulouse/Project/binding" class="page-nav-left" style="width:447px; float:right; display:block; text-align:right; text-decoration:none;
-
color:#666; font-size:18px;">Fungicides</br>
+
color:#666; font-size:18px;">Binding</br>
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
       <img src="https://static.igem.org/mediawiki/2014/e/ea/Template-igem2014-img-arrowright.png" style="display:block; float:right; padding-top:10px; " />
     </a>
     </a>

Latest revision as of 02:59, 18 October 2014