Team:Toulouse/Notebook/project-monitoring

From 2014.igem.org

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<br>Result : One good clone : PCR (check on 09/09/2014) and threonine test (check on 09/09/2014)</p>
<br>Result : One good clone : PCR (check on 09/09/2014) and threonine test (check on 09/09/2014)</p>
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<br>5. Binding Test
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<p class="title2">5. Binding Test</p>
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See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results#select2">here</a>
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<br> <h2 class="title1" id="select2">Chemotaxis</h2>
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<h2 class="title1" id="select2">Chemotaxis</h2>
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<br>1. Transformation of chemotaxis (Puc-57) into E. coli
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<p class="title2">1. Transformation of chemotaxis (Puc-57) into <i>E. coli</i></p>
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<br>   Gene chemotaxis synthesized by Eurofins, resuspended in 20μL Tris 10mM
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<p class="texte">Gene chemotaxis synthesized by Eurofins, resuspended in 20μL Tris 10mM
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<br>Date: 01/08/2014
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<br><b>Date:</b> 01/08/2014
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<br>Result: We obtained distinct colonies on LA + Ampicillin (100 µg/mL) plates  
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<br><b>Result:</b> We obtained distinct colonies on LA + Ampicillin (100 µg/mL) plates</p>
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<br>Culture of 4 clones: A, B, C, D of chemotaxis (Puc57) transformed into E. coli
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<p class="title3">Culture of 4 clones: A, B, C, D of chemotaxis (Puc57) transformed into <i>E. coli</i></p>
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  <br> Date: 04/08/2014
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<p class="texte"><b>Date:</b> 04/08/2014</p>
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<br>Miniprep with QIAprep Spin Miniprep Kit Using a Microcentrifuge : 4 clones of chemotaxis (Puc57) into E. coli
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<p class="title3">Miniprep with QIAprep Spin Miniprep Kit Using a Microcentrifuge: 4 clones of chemotaxis (Puc57) into <i>E. coli</i></p>
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<br>Date: 05/08/2014
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<p class="texte"><b>Date:</b> 05/08/2014
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<br>Result : 4*50µL of chemotaxis (Puc57) obtained
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<br><b>Result:</b> 4*50µL of chemotaxis (Puc57) obtained</p>
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<br>2. Cloning chemotaxis  BBa_K1364000 (chemotaxis_Puc57) with digested pSB1C3 with EcoRI and PstI into E. coli
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<p class="title2">2. Cloning chemotaxis  BBa_K1364000 (chemotaxis_Puc57) with digested pSB1C3 with EcoRI and PstI into <i>E. coli</i></p>
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<br>Digestion BBa_K1364000 (chemotaxis_Puc57) with EcoRI and PstI - PCR kit Clean up
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<p class="title3">Digestion BBa_K1364000 (chemotaxis_Puc57) with EcoRI and PstI - PCR kit Clean up</p>
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<br>Date: 07/08/2014
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<p class="texte"><b>Date:</b> 07/08/2014
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<br>Result: Expected band after digestion for BBa_K1364000 : 2300 bp
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<br><b>Result:</b> Expected band after digestion for BBa_K1364000: 2300 bp
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<br>Problem: We can't distinguish the vector band (2500 bp)
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<br><b>Problem:</b> We can't distinguish the vector band (2500 bp)</p>
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<br>•   Gel extraction of BBa_K1364000
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<p class="title3">Gel extraction of BBa_K1364000</p>
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<br>Date: 07/08/2014
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<p class="texte"><b>Date:</b> 07/08/2014</p>
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<br>Ligation BBa_K1364000 and digested PsB1C3 with EcoRI and PstI
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<p class="title3">Ligation BBa_K1364000 and digested PsB1C3 with EcoRI and PstI</p>
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<br>Date: 08/08/2014
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<p class="texte"><b>Date:</b> 08/08/2014</p>
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<br>Transformation BBa_K1364000 in E.coli
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<p class="title3">Transformation BBa_K1364000 in E.coli</p>
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<br>Date: 08/08/2014
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<p class="texte"><b>Date:</b> 08/08/2014
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<br>Result: We obtained distinct colonies on LA + Cm (15 µg/mL) plates and resuspended 15 colonies in LB+Cm (15 µg/mL)
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<br><b>Result:</b> We obtained distinct colonies on LA + Cm (15 µg/mL) plates and resuspended 15 colonies in LB+Cm (15 µg/mL)</p>
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<br>Test of sensibility on Ampicillin
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<p class="title3">Test of sensibility on Ampicillin</p>
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<br>Date: 10/08/2014
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<br><b>Date:</b> 10/08/2014
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<br>Result: we can determine which colonies are sensible for ampicillin and know which bacterium is carrying the chemotaxis gene.
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<br><b>Result:</b> we can determine which colonies are sensible for ampicillin and know which bacterium is carrying the chemotaxis gene.</p>
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<br>PCR
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<p class="title3">PCR</p>
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<br>Date: 11/08/2014
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<p class="texte"><b>Date:</b> 11/08/2014</p>
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<br>Digestion BBa_K1364000 on pSB1C3 with EcoRI and PstI
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<p class="title3">Digestion BBa_K1364000 on pSB1C3 with EcoRI and PstI</p>
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<br>Date: 11/08/2014
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<p class="texte"><b>Date:</b> 11/08/2014
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<br>Result : There is one colony which presents the right construction.
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<br><b>Result:</b> There is one colony which presents the right construction.</p>

Revision as of 15:23, 15 October 2014