Team:Toulouse/Notebook/Protocols

From 2014.igem.org

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<br>When the plasmid is integrated, the clone can grow on minimum medium with threonine and on LB + Spectinomycin but can not grow on the minimum medium without thronine.
<br>When the plasmid is integrated, the clone can grow on minimum medium with threonine and on LB + Spectinomycin but can not grow on the minimum medium without thronine.
Moreover, a colony PCR can be performed with the same protocol as previously presented. The used primers are VFBS and VRBS.
Moreover, a colony PCR can be performed with the same protocol as previously presented. The used primers are VFBS and VRBS.
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<center><img src="http://2014.igem.org/wiki/images/a/a3/Thr.png"></center>
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<p class="legend">Threnonine test (Left: MC Thr+; Right: MC Thr -)</p>
<p class="title1" id="select8">Final Tests</p>
<p class="title1" id="select8">Final Tests</p>

Revision as of 14:46, 17 October 2014