Team:Toulouse/Notebook/Protocols

From 2014.igem.org

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<br>- Put 200µl of the different chemoattractants in the wells of the ELISA plate and pipette 15µL of each with the multichannel pipette: galactose which represents our negative control, glucose which represents our positive control and N-acetylglucosamine (NAG).  
<br>- Put 200µl of the different chemoattractants in the wells of the ELISA plate and pipette 15µL of each with the multichannel pipette: galactose which represents our negative control, glucose which represents our positive control and N-acetylglucosamine (NAG).  
NB: The NAG is the most important test because it is the monosaccharide which composes the chitin on Ceratocystis platani wall. The volume in the tips must be marked.
NB: The NAG is the most important test because it is the monosaccharide which composes the chitin on Ceratocystis platani wall. The volume in the tips must be marked.
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<br>- After an hour, put the volume of the tips on parafilm.  
<br>- After an hour, put the volume of the tips on parafilm.  
<br>- Each solution is diluted 1/10,000 and 100µL is spread on LA medium.
<br>- Each solution is diluted 1/10,000 and 100µL is spread on LA medium.
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<br>- The plates are then incubated overnight at 37°C.
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<br>- The plates are then incubated overnight at 37°C.</p>
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Revision as of 17:08, 13 October 2014