Team:Toulouse/Notebook/Protocols

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<div class="Sub_title"> E. coli competent cells <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> E. coli competent cells</div>
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<div class="Sub_title"> E. coli transformation protocol <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> E. coli transformation protocol </div>
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<div class="Sub_title"> Miniprep <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> Miniprep </div>
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<div class="Sub_title"> Cloning <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> Cloning </div>
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<div class="Sub_title"> B. subtilis transformation <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> B. subtilis transformation </div>
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<div class="Sub_title"> Tests <a href="https://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<div class="Sub_title"> Tests </div>
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<br>- Mix and vortex.  
<br>- Mix and vortex.  
<br>- Place a drop of the mixture in a Thoma cell and count the number of conidia.  
<br>- Place a drop of the mixture in a Thoma cell and count the number of conidia.  
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<br><I>NB: You should count at least 5 squares (made of 4x4 squares, marked number 2 below:) and make an average. Multiply this number by 250.000 and you get the number of conodia per ml.</I> 
 
<br>- Make dilutions of the solution in order to get the right amount of conidia in each plate: 25.000 /plate and 5000 plate
<br>- Make dilutions of the solution in order to get the right amount of conidia in each plate: 25.000 /plate and 5000 plate
<br><I>NB: As we want to spread 200µl per plate you have to get a final concentration of 125.103 conidia / ml (and 25 000 conidia /ml, just make a dilution of the first solution to get this one).</I>
<br><I>NB: As we want to spread 200µl per plate you have to get a final concentration of 125.103 conidia / ml (and 25 000 conidia /ml, just make a dilution of the first solution to get this one).</I>

Revision as of 11:58, 2 October 2014