Team:Toulouse/Notebook/Protocols

From 2014.igem.org

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<br>The complete mixture should be dissolved in 100 ml. First add 50 ml milliQ water and mix. When everything is dissolved add MQ water till 100 ml. Filter sterilize the complete mixture and store at -20°C.
<br>The complete mixture should be dissolved in 100 ml. First add 50 ml milliQ water and mix. When everything is dissolved add MQ water till 100 ml. Filter sterilize the complete mixture and store at -20°C.
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<p class="title1" id="select7"> Checking of the genetic constructions after plasmid integration in <I>Bacillus subtilis</I> </p>
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<p class="title1" id="select7">Checking of the genetic constructions after plasmid integration in <I>Bacillus subtilis</I></p>
<p class="texte">
<p class="texte">
<br>Test of the pSBBS4S plasmid integration in Bacillus subtilis genome on the threonine site:
<br>Test of the pSBBS4S plasmid integration in Bacillus subtilis genome on the threonine site:
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<p class="title2">Chemotaxis test</p>
<p class="title2">Chemotaxis test</p>
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<p class="texte">
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<br>Many chemotaxis tests exist such as plate tests or capillary tests. We tried all of them and we decided to optimize the « Capillary essay » from Imperial College 2011 iGEM team.
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Many chemotaxis tests exist such as plate tests or capillary tests. We tried all of them and we decided to optimize the « Capillary essay » from Imperial College 2011 iGEM team.
<br>- Prepare the bacteria in LB medium so they reach an OD between 0.5 and 0.6 (exponential growth phase). This step takes about 5 hours.
<br>- Prepare the bacteria in LB medium so they reach an OD between 0.5 and 0.6 (exponential growth phase). This step takes about 5 hours.
<br>- Prepare the multichannel pipette: improve the cohesion between the tips and the pipette with Blu-Tack to avoid air and the possibility of leakage.
<br>- Prepare the multichannel pipette: improve the cohesion between the tips and the pipette with Blu-Tack to avoid air and the possibility of leakage.

Revision as of 22:55, 15 October 2014