Team:Toulouse/Notebook/Protocols

From 2014.igem.org

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<br>- 130µL of supernatant is used to soak a plug on a fungus plate. The bacterial pellet is resuspended in 130µL of LB medium and put on a tampon.  
<br>- 130µL of supernatant is used to soak a plug on a fungus plate. The bacterial pellet is resuspended in 130µL of LB medium and put on a tampon.  
<br>- The plates containing 10,000 conidia and the supernatant or bacteria soaked plugs are then put at room temperature for a few days according to the growth speed of the fungi. Controls are also realized with wilt type strains or copper sulfate at 10 and 20mg/mL.
<br>- The plates containing 10,000 conidia and the supernatant or bacteria soaked plugs are then put at room temperature for a few days according to the growth speed of the fungi. Controls are also realized with wilt type strains or copper sulfate at 10 and 20mg/mL.
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Revision as of 12:20, 9 October 2014