http://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&feed=atom&action=historyTeam:Toulouse/Notebook/Calendar - Revision history2024-03-28T16:18:14ZRevision history for this page on the wikiMediaWiki 1.16.5http://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=377540&oldid=prevDbby at 00:48, 18 October 20142014-10-18T00:48:10Z<p></p>
<a href="http://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=377540&oldid=376856">Show changes</a>Dbbyhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=376856&oldid=prevDbby at 00:42, 18 October 20142014-10-18T00:42:31Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:42, 18 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>January – June 2014: Projects brainstorming<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">January – June 2014: Projects brainstorming</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>June 2014: Choice of SubtiTree project<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">June 2014: Choice of SubtiTree project</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of May, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of May, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By this period, we evaluated our budget to approximately <B> €39,500 </B>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By this period, we evaluated our budget to approximately <B>€39,500</B>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">Collapse</a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 2); return false">Collapse</a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 1(16-22 June)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 1(16-22 June)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 2 (23-29 June)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 2 (23-29 June)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>July 2014<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">July 2014</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activities</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activities</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 274:</td>
<td colspan="2" class="diff-lineno">Line 274:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 3 (30 June -6 July) and Week 4 (7-13 July)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 3 (30 June -6 July) and Week 4 (7-13 July)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 301:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 5 (14-20 July)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 5 (14-20 July)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 327:</td>
<td colspan="2" class="diff-lineno">Line 327:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 6 (21 July-27 July)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 6 (21 July-27 July)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 352:</td>
<td colspan="2" class="diff-lineno">Line 352:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>August 2014<del class="diffchange diffchange-inline"></p> </del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">August 2014</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activities</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activities</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 363:</td>
<td colspan="2" class="diff-lineno">Line 363:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 7 (28 July-3 August)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 7 (28 July-3 August)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 407:</td>
<td colspan="2" class="diff-lineno">Line 407:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 8 (04-10 August)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 8 (04-10 August)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 447:</td>
<td colspan="2" class="diff-lineno">Line 447:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 9 (11-17 August)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 9 (11-17 August)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 475:</td>
<td colspan="2" class="diff-lineno">Line 475:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 10 (18-24 August)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 10 (18-24 August)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 504:</td>
<td colspan="2" class="diff-lineno">Line 504:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 11 (25- 31 August)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 11 (25- 31 August)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 542:</td>
<td colspan="2" class="diff-lineno">Line 542:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>September 2014<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">September 2014</div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class ="thelanguage"><p class="title2">Main activities</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class ="thelanguage"></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="title2">Main activities</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Finish the tests of the different modules</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Finish the tests of the different modules</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 556:</td>
<td colspan="2" class="diff-lineno">Line 557:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">Collapse</a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">Collapse</a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 12 (1-7 September)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 12 (1-7 September)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 588:</td>
<td colspan="2" class="diff-lineno">Line 589:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 13 (8- 14 September)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 13 (8- 14 September)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 620:</td>
<td colspan="2" class="diff-lineno">Line 621:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 14 (15 - 21 September)<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 14 (15 - 21 September)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 655:</td>
<td colspan="2" class="diff-lineno">Line 656:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"<del class="diffchange diffchange-inline">><p class="title3"; "line-height:6px"; "padding:10px";</del>>Week 15 (22 - 28 September)</div<del class="diffchange diffchange-inline">></p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2">Week 15 (22 - 28 September)</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 681:</td>
<td colspan="2" class="diff-lineno">Line 682:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>October 2014<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">October 2014</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activites</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activites</p></div></td></tr>
</table>Dbbyhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=376073&oldid=prevDbby at 00:35, 18 October 20142014-10-18T00:35:41Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 00:35, 18 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology"<del class="diffchange diffchange-inline">><p class="title2"; "line-height:6px"; "padding:10px";</del>>December 2013 – January 2014: Team selection<del class="diffchange diffchange-inline"></p></del></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology">December 2013 – January 2014: Team selection</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Constitution of the <a href="https://2014.igem.org/Team:Toulouse/Team">team</a> by interviewing different students from Université Toulouse III Paul Sabatier and INSA de Toulouse</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Constitution of the <a href="https://2014.igem.org/Team:Toulouse/Team">team</a> by interviewing different students from Université Toulouse III Paul Sabatier and INSA de Toulouse</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team ! </B></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team!</B></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Dbbyhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=355027&oldid=prevManon at 21:16, 17 October 20142014-10-17T21:16:30Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:16, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><script type='text/javascript' src='http://ajax.googleapis.com/ajax/libs/jquery/1.9.0/jquery.min.js'></script></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><script type='text/javascript' src='http://ajax.googleapis.com/ajax/libs/jquery/1.9.0/jquery.min.js'></script></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><script type='text/javascript'> $(function(){ </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><script type='text/javascript'> $(function(){ <ins class="diffchange diffchange-inline">modelling</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $(window).scroll(function () {</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $(window).scroll(function () {</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> if ($(this).scrollTop() > 250) {</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> if ($(this).scrollTop() > 250) {</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 566:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Chemotaxis test and <del class="diffchange diffchange-inline">modelling</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Chemotaxis test and <ins class="diffchange diffchange-inline">modeling</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Fungicides tests on EcAMP-A, EcAMP-B, EcAMP-C, EcAMP-D, MaxiOpA, MaxiOpB, MaxiOpC, MaxiOpD from 2ml of overnight cultures. Only with 25000 conidia with both fungi.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Fungicides tests on EcAMP-A, EcAMP-B, EcAMP-C, EcAMP-D, MaxiOpA, MaxiOpB, MaxiOpC, MaxiOpD from 2ml of overnight cultures. Only with 25000 conidia with both fungi.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- PCR on <del class="diffchange diffchange-inline">pSBBS4S </del>+ BBa_K1162001 (EcAMP) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- PCR on <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>+ BBa_K1162001 (EcAMP) </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: failed on both diluted plasmid and colony</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: failed on both diluted plasmid and colony</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR on D4E1 colony with different primers</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR on D4E1 colony with different primers</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Transformation of K1364014+K1364006 in <i>E. coli</i> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Transformation of K1364014 + K1364006 in <i>E. coli</i> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Transformation of GAFP1 + D4E1, GAFP1 and EcAMP+GAFP1+D4E1 on <i>B. subtilis</i> + colony PCR + threonine test</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Transformation of GAFP1 + D4E1, GAFP1 and EcAMP + GAFP1 + D4E1 on <i>B. subtilis</i> + colony PCR + threonine test</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1 <del class="diffchange diffchange-inline">CA </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/<ins class="diffchange diffchange-inline">><i</ins>>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1<ins class="diffchange diffchange-inline"></i></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Cloning of K606013+pEX_K4, 823022+<del class="diffchange diffchange-inline">PlepA_RFP</del>, 823022+ <del class="diffchange diffchange-inline">Pveg_RFP</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Cloning of K606013 + pEX_K4, 823022 + <ins class="diffchange diffchange-inline">P<sub>lepA</sub>_RFP</ins>, 823022 + <ins class="diffchange diffchange-inline">P<sub>veg</sub>_RFP</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Chemotaxis tests: different times of LB + agar addition in the LB medium (after 3 minutes, 5 minutes, 8 minutes, 10 minutes…) are performed => No difference of the results after spreading on the plates.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Chemotaxis tests: different times of LB + agar addition in the LB medium (after 3 minutes, 5 minutes, 8 minutes, 10 minutes…) are performed => No difference of the results after spreading on the plates.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- New test of chemotaxis: capillary between two Eppendorf tubes</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- New test of chemotaxis: capillary between two Eppendorf tubes</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 624:</td>
<td colspan="2" class="diff-lineno">Line 624:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Support of two French city halls: Mairie de Montréal<del class="diffchange diffchange-inline">, </del>Mairie de Ventenac en Minervois.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Support of two French city halls: Mairie de Montréal <ins class="diffchange diffchange-inline">and </ins>Mairie de Ventenac en Minervois.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- First interview by the French national radio France Inter which will be aired on October <del class="diffchange diffchange-inline">12th</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- First interview by the French national radio France Inter which will be aired on October <ins class="diffchange diffchange-inline">19<sup>th</sup></ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Beginning of the organization of the Toulouse iGEM team acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Beginning of the organization of the Toulouse iGEM team acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Final design of the wiki</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Final design of the wiki</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 632:</td>
<td colspan="2" class="diff-lineno">Line 632:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1-D4E1)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1 - D4E1)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Chemotaxis test with wild type <i>B. subtilis</i> strain using Imperial College protocol</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Chemotaxis test with wild type <i>B. subtilis</i> strain using Imperial College protocol</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Tansformation of BBa_K1364011 + BBa_K823022 (<del class="diffchange diffchange-inline">pSBBS4S</del>) in <i>B. subtilis</i></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Tansformation of BBa_K1364011 + BBa_K823022 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>) in <i>B. subtilis</i></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture of fungi with fungicides</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture of fungi with fungicides</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Sequencing of plasmids containing the fungicides</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Sequencing of plasmids containing the fungicides</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="title3">Weekly meeting with the instructors(09/18/2014)</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="title3">Weekly meeting with the instructors (09/18/2014)</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- The next step is to sequence the maxi operon (EcAMP1 – GAFP1 – D4E1).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- The next step is to sequence the maxi operon (EcAMP1 – GAFP1 – D4E1).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Try different approaches for the chemotaxis:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Try different approaches for the chemotaxis:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/><del class="diffchange diffchange-inline">- </del>New protocol with the capillary assay with a new system made by a glassblower.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>New protocol with the capillary assay with a new system made by a glassblower.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/><del class="diffchange diffchange-inline">- </del>Make the Imperial College test again by testing 3 different solutions: chitin, N-acetylglucosamine and another carbon source.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>Make the Imperial College test again by testing 3 different solutions: chitin, N-acetylglucosamine and another carbon source.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Decrease the number of conidia and temperature for the fungicide test.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Decrease the number of conidia and temperature for the fungicide test.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 655:</td>
<td colspan="2" class="diff-lineno">Line 655:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 15 (22 - 28 September)</div></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 15 (22 - 28 September)</div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- New support of Sallèles d’Aude<del class="diffchange diffchange-inline">, Ventenac en Minervois, </del>Labastide d'Anjou<del class="diffchange diffchange-inline">, Montréal </del>city halls but also the Département de l'Hérault.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- New support of Sallèles d’Aude <ins class="diffchange diffchange-inline">and </ins>Labastide d'Anjou city halls but also the Département de l'Hérault.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Payment of the plane tickets.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Payment of the plane tickets.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Acknowledgement day (<del class="diffchange diffchange-inline">4th </del>December): contact with catering service, reservation of the amphitheater, list of guests…</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Acknowledgement day (<ins class="diffchange diffchange-inline">4<sup>th</sup> </ins>December): contact with catering service, reservation of the amphitheater, list of guests…</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Order of the T shirt for the Jamboree! We look awesome with them!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Order of the T shirt for the Jamboree! We look awesome with them!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 685:</td>
<td colspan="2" class="diff-lineno">Line 685:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activites</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title2">Main activites</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Preparation of the wiki every day... all day long...so hard to work on it, especially at night!</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Preparation of the wiki every day... all day long... so hard to work on it, especially at night!</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Preparation of the poster and the presentation for the Giant Jamboree with our instructors </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Preparation of the poster and the presentation for the Giant Jamboree with our instructors </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
</table>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=354359&oldid=prevManon at 21:09, 17 October 20142014-10-17T21:09:10Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:09, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Subculture and transformation in <i> B. subtilis </i> of BBa_K1364011 in <del class="diffchange diffchange-inline">pSBBS4S </del>(BBa_K823022) and cloning of BBa_K1364011 on <del class="diffchange diffchange-inline">pSBBS1C </del>(BBa_K823023).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Subculture and transformation in <i> B. subtilis </i> of BBa_K1364011 in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>(BBa_K823022) and cloning of BBa_K1364011 on <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C </ins>(BBa_K823023).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Problem: no clone had the insert on <del class="diffchange diffchange-inline">pSBBS1C </del>=> the cloning had to be done again</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Problem: no clone had the insert on <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C </ins>=> the cloning had to be done again</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Miniprep of BBa_K1364011 in BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>) + Transformation in <i>B. subtilis</i>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins>- Miniprep of BBa_K1364011 in BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>) + Transformation in <i>B. subtilis</i>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) , BBA_1364002 (GAFP1) , BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) on BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) , BBA_1364002 (GAFP1) , BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) on BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>) </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation BBa_1364004 (in <del class="diffchange diffchange-inline">pSBBS4S </del>in <i>E. coli</i> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation BBa_1364004 (in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>in <i>E. coli</i> <ins class="diffchange diffchange-inline">)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 (<del class="diffchange diffchange-inline">Pveg </del>+ RBS SpoVG + EcAMP + GAFP1 +D4E1 + double terminator) in K823022 (<del class="diffchange diffchange-inline">pSBBS4S</del>) and in BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>), miniprep and digestion by restriction enzymes to see the size of the insert on a 1% gel</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 (<ins class="diffchange diffchange-inline">P<sub>veg</sub> </ins>+ RBS SpoVG + EcAMP + GAFP1 + D4E1 + double terminator) in K823022 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>) and in BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>), miniprep and digestion by restriction enzymes to see the size of the insert on a 1% gel</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Problem: no clone had the insert on <del class="diffchange diffchange-inline">pSBBS1C </del>=> the cloning had to be done again</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Problem: no clone had the insert on <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C </ins>=> the cloning had to be done again</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<td colspan="2" class="diff-lineno">Line 523:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSB<sub>BS</sub>1C) and transformation in <i>Bacillus subtilis</i> strain. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSB<sub>BS</sub>1C) and transformation in <i>Bacillus subtilis</i> strain. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Fungicide test of BBa_K1364011 in <del class="diffchange diffchange-inline">pSBBs4S</del>, Promotor + BBA_1364002 (GAFP1) + Terminator (<del class="diffchange diffchange-inline">biobrick </del>BBa_K1364008) in <del class="diffchange diffchange-inline">pSBBS4S</del>, Promotor + BBA_1364003 (D4E1) + Terminator (BioBrick BBa_K1364009) in pSB<sub>BS</sub>4S, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1) + Terminator (BioBrick BBa_K1364013) in pSB<sub>BS</sub>4S</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Fungicide test of BBa_K1364011 in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>, Promotor + BBA_1364002 (GAFP1) + Terminator (<ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364008) in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>, Promotor + BBA_1364003 (D4E1) + Terminator (BioBrick BBa_K1364009) in pSB<sub>BS</sub>4S, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1) + Terminator (BioBrick BBa_K1364013) in pSB<sub>BS</sub>4S</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of a new vector PKL 190 (threonine integrative) in <i>Bacillus</i> strain </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of a new vector PKL 190 (threonine integrative) in <i>Bacillus</i> strain </div></td></tr>
</table>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=353972&oldid=prevManon at 21:04, 17 October 20142014-10-17T21:04:55Z<p></p>
<table style="background-color: white; color:black;">
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<col class='diff-marker' />
<col class='diff-content' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:04, 17 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 182:</td>
<td colspan="2" class="diff-lineno">Line 182:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Thanks to weekly meetings, our team was able to discuss about new project ideas.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Thanks to weekly meetings, our team was able to discuss about new project ideas.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Many ideas were approached and debated regarding the feasibility thanks to the <del class="diffchange diffchange-inline">litterature </del>and supervisors including teachers and researchers.<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Many ideas were approached and debated regarding the feasibility thanks to the <ins class="diffchange diffchange-inline">literature </ins>and supervisors including teachers and researchers.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a list of the main projects: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a list of the main projects: </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 312:</td>
<td colspan="2" class="diff-lineno">Line 312:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- GFP and RFP digestion amplified by miniprep and gel electrophoresis <br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- GFP and RFP digestion amplified by miniprep and gel electrophoresis <br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: the digested GFP doesn’t appear on the gel contrary to RFP plasmid. We assumed a problem with the GFP miniprep. We tried the miniprep from the INSA Toulouse team from 2013 and the GFP plasmid was fully digested.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: the digested GFP doesn’t appear on the gel contrary to RFP plasmid. We assumed a problem with the GFP miniprep. We tried the miniprep from the INSA Toulouse team from 2013 and the GFP plasmid was fully digested.<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation and cryopreservation of <del class="diffchange diffchange-inline">biobricks </del>BBa_K823002 (<del class="diffchange diffchange-inline">PlepA</del>), BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>), BBa_K606061 (RBS SpoVG) and BBa_B0015 (double terminator), BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>), BBa_K733013 (<del class="diffchange diffchange-inline">Pveg</del>+ RBS) in <i>E. coli</i>.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation and cryopreservation of <ins class="diffchange diffchange-inline">BioBricks </ins>BBa_K823002 (<ins class="diffchange diffchange-inline">P<sub>lep</sub>A</ins>), BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>), BBa_K606061 (RBS SpoVG) and BBa_B0015 (double terminator), BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>), BBa_K733013 (<ins class="diffchange diffchange-inline">P<sub>veg</sub> </ins>+ RBS) in <i>E. coli</i>.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Munich <i>B. subtilis</i> backbones<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Munich <i>B. subtilis</i> backbones<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K606061 (RBS SpoVG) with BBa_K823003 (<del class="diffchange diffchange-inline">PvEG</del>) and BBa_K823002 (<del class="diffchange diffchange-inline">PlepA</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K606061 (RBS SpoVG) with BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) and BBa_K823002 (<ins class="diffchange diffchange-inline">P<sub>lepA</sub></ins>)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 320:</td>
<td colspan="2" class="diff-lineno">Line 320:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Eurofins genes<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Eurofins genes<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Start assembling the <del class="diffchange diffchange-inline">biobricks </del>for the fungicides<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Start assembling the <ins class="diffchange diffchange-inline">BioBricks </ins>for the fungicides<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Check all the cloning</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Check all the cloning</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 339:</td>
<td colspan="2" class="diff-lineno">Line 339:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR and migration on electrophoresis gel<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR and migration on electrophoresis gel<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation BBA_1364003 (D4E1) on pSB1C3 and BBA_1364002 (GAFP1) on pSB1C3<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation BBA_1364003 (D4E1) on pSB1C3 and BBA_1364002 (GAFP1) on pSB1C3<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning BBA_1364002 (GAFP1) with BBa_B0015 (double terminator) and BBA_1364003 (D4E1) with BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning BBA_1364002 (GAFP1) with BBa_B0015 (double terminator) and BBA_1364003 (D4E1) with BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 374:</td>
<td colspan="2" class="diff-lineno">Line 374:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work: </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work: </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) + RFP and BBa_K823002 (<del class="diffchange diffchange-inline">PlepA</del>) + RFP in <del class="diffchange diffchange-inline">pSBBs1C</del><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) + RFP and BBa_K823002 (<ins class="diffchange diffchange-inline">P<sub>lepA</sub></ins>) + RFP in <ins class="diffchange diffchange-inline">pSB<sub>Bs</sub>1C</ins><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Test of every <del class="diffchange diffchange-inline">pSBBs </del>vector : BBa_K823021 (<del class="diffchange diffchange-inline">pSBBS1C</del>-lacZ), BBa_K823022 (<del class="diffchange diffchange-inline">pSBBS4S</del>), BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>), minipreps and cryopreservation of the best clones<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Test of every <ins class="diffchange diffchange-inline">pSB<sub>BS</sub> </ins>vector: BBa_K823021 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>-lacZ), BBa_K823022 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>), BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>), minipreps and cryopreservation of the best clones<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Checking of BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) transformants (<del class="diffchange diffchange-inline">biobrick </del>BBa_K1364007) and BBA_1364003 (D4E1) + BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) <del class="diffchange diffchange-inline">biobrick </del>BBa_K1364009</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Checking of BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) transformants (<ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364007) and BBA_1364003 (D4E1) + BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) <ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364009</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) + <del class="diffchange diffchange-inline">pSBBS4S </del>(BBa_K823022) and subculture of the colonies<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) + <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>(BBa_K823022) and subculture of the colonies<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K823003 (Pveg) + BBA_1364003 (D4E1) with BBa_B0015 (double terminator)<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K823003 (Pveg) + BBA_1364003 (D4E1) with BBa_B0015 (double terminator)<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K823003 (Pveg) + RFP (BBa_K1364017) in <del class="diffchange diffchange-inline">pSBBS4S </del>(BBa_K823022), subculture and minipreps<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K823003 (Pveg) + RFP (BBa_K1364017) in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>(BBa_K823022), subculture and minipreps<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K823002 (PlepA) + RFP (BBa_K1364016) in <del class="diffchange diffchange-inline">pSBBs4S </del>(BBa_K823022), subculture and minipreps<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K823002 (PlepA) + RFP (BBa_K1364016) in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>(BBa_K823022), subculture and minipreps<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_1364018 (Strong Promotor + RFP)<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_1364018 (Strong Promotor + RFP)<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) + Promotor BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) <del class="diffchange diffchange-inline">biobrick </del>BBa_K1364012 with gel extraction for BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) and a PCR cleanup for BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>), subculture<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) + Promotor BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) <ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364012 with gel extraction for BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) and a PCR cleanup for BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>), subculture<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning BBa_K823003 (Pveg) + BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) on pSBBS4S in <i>E. coli</i><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning BBa_K823003 (Pveg) + BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) on pSBBS4S in <i>E. coli</i><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of binding gene with <i>E. coli</i> competent cells<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of binding gene with <i>E. coli</i> competent cells<br/></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 393:</td>
<td colspan="2" class="diff-lineno">Line 393:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="title4">Weekly meeting with the instructors(08/01/2014)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="title4">Weekly meeting with the instructors (08/01/2014)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Possible problem with the restriction enzymes regarding the digestion: new recommendations</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Possible problem with the restriction enzymes regarding the digestion: new recommendations</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 415:</td>
<td colspan="2" class="diff-lineno">Line 415:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Check the cloning of BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>)+BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) on <del class="diffchange diffchange-inline">pSBBs4S </del>and cryopreservation</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Check the cloning of BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>)+BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) on <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>and cryopreservation</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation of BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) +BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) (<del class="diffchange diffchange-inline">biobrick </del>BBa_ K1364008) in BBa_K823022 (<del class="diffchange diffchange-inline">pSBBS4S</del>) </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation of BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) + BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) (<ins class="diffchange diffchange-inline">BioBrick </ins>BBa_ K1364008) in BBa_K823022 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>) </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364001, the binding gene + BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) on pSB1C3 and <del class="diffchange diffchange-inline">pSBBS </del>4S</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364001, the binding gene + BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) on pSB1C3 and <ins class="diffchange diffchange-inline">pSB<sub>BS</sub></ins>4S</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Problem: the band is at <del class="diffchange diffchange-inline">1500 bp </del>instead of <del class="diffchange diffchange-inline">1300 bp </del>on the gel</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Problem: the band is at <ins class="diffchange diffchange-inline">1500bp </ins>instead of <ins class="diffchange diffchange-inline">1300bp </ins>on the gel</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of BBa_K1162001 (EcAMP) + BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) + BBa_K606061 (RBS SpoVG), miniprep, PCR and digestion </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of BBa_K1162001 (EcAMP) + BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) + BBa_K606061 (RBS SpoVG), miniprep, PCR and digestion </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Problem: the fragment of DNA is too small (<del class="diffchange diffchange-inline">149 bp</del>) to be seen on the gel and was probably lost during the PCR cleanup. Instead of that, we used the heat enzymes inactivation at 95°C for 15 minutes. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Problem: the fragment of DNA is too small (<ins class="diffchange diffchange-inline">149bp</ins>) to be seen on the gel and was probably lost during the PCR cleanup. Instead of that, we used the heat enzymes inactivation at 95°C for 15 minutes. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning of Promotor + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) in pSB1C3 and <del class="diffchange diffchange-inline">pSBBS4S </del>and cryopreservation</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning of Promotor + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) in pSB1C3 and <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S </ins>and cryopreservation</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Elaboration of an efficient fungicide test protocol </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Elaboration of an efficient fungicide test protocol </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 460:</td>
<td colspan="2" class="diff-lineno">Line 460:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Chemotaxis test with different concentrations of glucose on a 0.3% agar.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Chemotaxis test with different concentrations of glucose on a 0.3% agar.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Miniprep of <del class="diffchange diffchange-inline">pSBBs4S </del>with binding gene and transformation in <i>B. subtilis</i>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Miniprep of <ins class="diffchange diffchange-inline">pSB<sub>B</sub>4S </ins>with binding gene and transformation in <i>B. subtilis</i>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no digestion was visible on the gel => the cloning failed</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no digestion was visible on the gel => the cloning failed</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation of BBa_K1374010 (<del class="diffchange diffchange-inline">Pveg </del>+ RBS SpoVG + EcAMP) + BBa_K1364012 (RBS + <del class="diffchange diffchange-inline">Gafp1 </del>+ D4E1 + double terminator), subculture</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation of BBa_K1374010 (<ins class="diffchange diffchange-inline">P<sub>veg</sub> </ins>+ RBS SpoVG + EcAMP) + BBa_K1364012 (RBS + <ins class="diffchange diffchange-inline">GAFP1 </ins>+ D4E1 + double terminator), subculture</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Subculture of <i>B. subtilis</i> clones transformed by BBa_K823003 (<del class="diffchange diffchange-inline">Pveg</del>) + BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) in <del class="diffchange diffchange-inline">pSBBs4S</del>, cryopreservation</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Subculture of <i>B. subtilis</i> clones transformed by BBa_K823003 (<ins class="diffchange diffchange-inline">P<sub>veg</sub></ins>) + BBA_1364002 (GAFP1) + BBa_B0015 (double terminator) in <ins class="diffchange diffchange-inline">pSB<sub>B</sub>4S</ins>, cryopreservation</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Fungicide test</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Fungicide test</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 515:</td>
<td colspan="2" class="diff-lineno">Line 515:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 in BBa_K823022 and in BBa_K823023 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 in BBa_K823022 and in BBa_K823023 </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Cloning GAFP1 + BBa_K823023 (<del class="diffchange diffchange-inline">pSBBS1C</del>) in <i>B. subtilis</i> with a change in the protocol: pre-induction of the culture with 0.125 µg/ml of chloramphenicol one hour after the addition of DNA, and let grow for another hour.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Cloning GAFP1 + BBa_K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>) in <i>B. subtilis</i> with a change in the protocol: pre-induction of the culture with 0.125 µg/ml of chloramphenicol one hour after the addition of DNA, and let grow for another hour.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no colony </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no colony </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 521:</td>
<td colspan="2" class="diff-lineno">Line 521:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Liquid culture of <i>Bacillus subtilis</i> + BBA_1364002 (GAFP1); <i>Bacillus subtilis</i> + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) ; <i>Bacillus subtilis</i> + BBA_1364003 (D4E1) for future fungicide tests</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Liquid culture of <i>Bacillus subtilis</i> + BBA_1364002 (GAFP1); <i>Bacillus subtilis</i> + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) ; <i>Bacillus subtilis</i> + BBA_1364003 (D4E1) for future fungicide tests</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (<del class="diffchange diffchange-inline">pSBBs1C</del>) and transformation in <i>Bacillus subtilis</i> strain. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (<ins class="diffchange diffchange-inline">pSB<sub>BS</sub>1C</ins>) and transformation in <i>Bacillus subtilis</i> strain. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Fungicide test of BBa_K1364011 in pSBBs4S, Promotor + BBA_1364002 (GAFP1) + Terminator (biobrick BBa_K1364008) in pSBBS4S, Promotor + BBA_1364003 (D4E1) + Terminator (<del class="diffchange diffchange-inline">biobrick </del>BBa_K1364009) in <del class="diffchange diffchange-inline">pSBBS4S</del>, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1) + Terminator ( <del class="diffchange diffchange-inline">biobrick </del>BBa_K1364013) in <del class="diffchange diffchange-inline">pSBBS4S</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Fungicide test of BBa_K1364011 in pSBBs4S, Promotor + BBA_1364002 (GAFP1) + Terminator (biobrick BBa_K1364008) in pSBBS4S, Promotor + BBA_1364003 (D4E1) + Terminator (<ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364009) in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins>, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1) + Terminator (<ins class="diffchange diffchange-inline">BioBrick </ins>BBa_K1364013) in <ins class="diffchange diffchange-inline">pSB<sub>BS</sub>4S</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of a new vector PKL 190 (threonine integrative) in <i>Bacillus</i> strain </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of a new vector PKL 190 (threonine integrative) in <i>Bacillus</i> strain </div></td></tr>
</table>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=351137&oldid=prevManon at 20:33, 17 October 20142014-10-17T20:33:30Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:33, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- iGEM competition presentation and explanations<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- iGEM competition presentation and explanations<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Constitution of the team by interviewing different students from Université Paul Sabatier and INSA</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Constitution of the <ins class="diffchange diffchange-inline"><a href="https://2014.igem.org/Team:Toulouse/Team"></ins>team<ins class="diffchange diffchange-inline"></a> </ins>by interviewing different students from Université <ins class="diffchange diffchange-inline">Toulouse III </ins>Paul Sabatier and INSA <ins class="diffchange diffchange-inline">de Toulouse</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team ! </B></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team ! </B></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 182:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Thanks to weekly meetings, our team was able to discuss about new project ideas.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Thanks to weekly meetings, our team was able to discuss about new project ideas.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Many ideas were approached and debated regarding the feasibility thanks to the <del class="diffchange diffchange-inline">literature </del>and supervisors including teachers and researchers</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Many ideas were approached and debated regarding the feasibility thanks to the <ins class="diffchange diffchange-inline">litterature </ins>and supervisors including teachers and researchers<ins class="diffchange diffchange-inline">.<br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a list of the main projects: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This is a list of the main projects: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology"><p class="title2"; "line-height:6px"; "padding:10px";>June 2014: Choice of SubtiTree project</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology"><p class="title2"; "line-height:6px"; "padding:10px";>June 2014: Choice of SubtiTree project</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of <del class="diffchange diffchange-inline">June</del>, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of <ins class="diffchange diffchange-inline">May</ins>, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By this period, we evaluated our budget to approximately <B> <del class="diffchange diffchange-inline">k€ 40</del>,<del class="diffchange diffchange-inline">000 </del></B>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By this period, we evaluated our budget to approximately <B> <ins class="diffchange diffchange-inline">€39</ins>,<ins class="diffchange diffchange-inline">500 </ins></B>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Beginning of the laboratory work to create our optimized bacterium <br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Beginning of the laboratory work to create our optimized bacterium <br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Research of sponsors and <del class="diffchange diffchange-inline">the </del>communication thanks to the press</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Research of sponsors and communication thanks to the press</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Participation at the BioSynSys conferences: presentation of SubtiTree<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Participation at the BioSynSys conferences: presentation of SubtiTree <ins class="diffchange diffchange-inline">project</ins><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- We have put in place a newsletter system the first <del class="diffchange diffchange-inline">day </del>of each month to keep all our sponsors and people who support us updated about the project<del class="diffchange diffchange-inline">, our </del>financial <del class="diffchange diffchange-inline">state</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- We have put in place a newsletter system the first <ins class="diffchange diffchange-inline">friday </ins>of each month to keep all our sponsors and people who support us updated about the project <ins class="diffchange diffchange-inline">(</ins>financial<ins class="diffchange diffchange-inline">, scientific and communication aspects)</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Problem: A problem was reported in the use of the CYP. Indeed, the <del class="diffchange diffchange-inline">biobrick </del>is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Problem: A problem was reported in the use of the CYP. Indeed, the <ins class="diffchange diffchange-inline">BioBrick </ins>is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of <i>Bacillus</i> strain in a medium composed of many different carbon sources.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of <i>Bacillus</i> strain in a medium composed of many different carbon sources.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Competent cells and transformation practice using GFP and RFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Competent cells and transformation practice using GFP and RFP.</div></td></tr>
</table>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=350299&oldid=prevManon at 20:25, 17 October 20142014-10-17T20:25:22Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:25, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- E. coli transformation with BBA_J004450 (pSB1C3)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>transformation with BBA_J004450 (pSB1C3)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: A problem was reported in the use of the CYP. Indeed, the biobrick is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: A problem was reported in the use of the CYP. Indeed, the biobrick is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of Bacillus strain in a medium composed of many different carbon sources.<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of <ins class="diffchange diffchange-inline"><i></ins>Bacillus<ins class="diffchange diffchange-inline"></i> </ins>strain in a medium composed of many different carbon sources.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Competent cells and transformation practice using GFP and RFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Competent cells and transformation practice using GFP and RFP.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: the digested GFP doesn’t appear on the gel contrary to RFP plasmid. We assumed a problem with the GFP miniprep. We tried the miniprep from the INSA Toulouse team from 2013 and the GFP plasmid was fully digested.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: the digested GFP doesn’t appear on the gel contrary to RFP plasmid. We assumed a problem with the GFP miniprep. We tried the miniprep from the INSA Toulouse team from 2013 and the GFP plasmid was fully digested.<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation and cryopreservation of biobricks BBa_K823002 (PlepA), BBa_K823003 (Pveg), BBa_K606061 (RBS SpoVG) and BBa_B0015 (double terminator), BBa_K823023 (pSBBS1C), BBa_K733013 (Pveg+ RBS) in <i>E. coli</i>.<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation and cryopreservation of biobricks BBa_K823002 (PlepA), BBa_K823003 (Pveg), BBa_K606061 (RBS SpoVG) and BBa_B0015 (double terminator), BBa_K823023 (pSBBS1C), BBa_K733013 (Pveg+ RBS) in <i>E. coli</i>.<br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation of the Munich B. subtilis backbones<br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation of the Munich <ins class="diffchange diffchange-inline"><i></ins>B. subtilis<ins class="diffchange diffchange-inline"></i> </ins>backbones<br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K606061 (RBS SpoVG) with BBa_K823003 (PvEG) and BBa_K823002 (PlepA)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K606061 (RBS SpoVG) with BBa_K823003 (PvEG) and BBa_K823002 (PlepA)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 7 (28 July-3 August)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 7 (28 July-3 August)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 8 (04-10 August)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 8 (04-10 August)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 9 (11-17 August)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 9 (11-17 August)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 10 (18-24 August)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 10 (18-24 August)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) , BBA_1364002 (GAFP1) , BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) on BBa_K823023 (pSBBS1C) </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBA_1364003 (D4E1) , BBA_1364002 (GAFP1) , BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) on BBa_K823023 (pSBBS1C) </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Transformation BBa_1364004 (in pSBBS4S in E.coli </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Transformation BBa_1364004 (in pSBBS4S in <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 (Pveg + RBS SpoVG + EcAMP + GAFP1 +D4E1 + double terminator) in K823022 (pSBBS4S) and in BBa_K823023 (pSBBS1C), miniprep and digestion by restriction enzymes to see the size of the insert on a 1% gel</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Cloning of BBa_K1364014 (Pveg + RBS SpoVG + EcAMP + GAFP1 +D4E1 + double terminator) in K823022 (pSBBS4S) and in BBa_K823023 (pSBBS1C), miniprep and digestion by restriction enzymes to see the size of the insert on a 1% gel</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 11 (25- 31 August)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 11 (25- 31 August)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 519:</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no colony </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Problem: no colony </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- Liquid culture of Bacillus + BBA_1364002 (GAFP1); Bacillus + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) ; Bacillus + BBA_1364003 (D4E1) for future fungicide tests</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- Liquid culture of <ins class="diffchange diffchange-inline"><i></ins>Bacillus <ins class="diffchange diffchange-inline">subtilis</i> </ins>+ BBA_1364002 (GAFP1); <ins class="diffchange diffchange-inline"><i></ins>Bacillus <ins class="diffchange diffchange-inline">subtilis</i> </ins>+ BBA_1364003 (D4E1) + BBA_1364002 (GAFP1) ; <ins class="diffchange diffchange-inline"><i></ins>Bacillus <ins class="diffchange diffchange-inline">subtilis</i> </ins>+ BBA_1364003 (D4E1) for future fungicide tests</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSBBs1C) and transformation in <i>Bacillus subtilis</i> strain. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSBBs1C) and transformation in <i>Bacillus subtilis</i> strain. </div></td></tr>
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<td colspan="2" class="diff-lineno">Line 556:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">Collapse</a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:right;font-size:1.3em;"><a href="#" class="collapseLink" onClick="ddaccordion.collapseone('technology', 5); return false">Collapse</a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 12 (1-7 September)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 12 (1-7 September)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 588:</td>
<td colspan="2" class="diff-lineno">Line 588:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 13 (8- 14 September)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 13 (8- 14 September)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 599:</td>
<td colspan="2" class="diff-lineno">Line 599:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR on D4E1 colony with different primers</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- PCR on D4E1 colony with different primers</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Culture and miniprep of EcAMP to get more DNA + analytic digestion </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br/>- Transformation of K1364014+K1364006 in E. coli </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br/>- Transformation of K1364014+K1364006 in <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Transformation of GAFP1 + D4E1, GAFP1 and EcAMP+GAFP1+D4E1 on <i>B. subtilis</i> + colony PCR + threonine test</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>- Transformation of GAFP1 + D4E1, GAFP1 and EcAMP+GAFP1+D4E1 on <i>B. subtilis</i> + colony PCR + threonine test</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1 CA </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br/>NB: good results for the maxi operon and GAFP1 but not for GAFP1 + D4E1 CA </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div class="technology2"><p class="<del class="diffchange diffchange-inline">title2</del>"; "line-height:6px"; "padding:10px";>Week 14 (15 - 21 September)</p></div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div class="technology2"><p class="<ins class="diffchange diffchange-inline">title3</ins>"; "line-height:6px"; "padding:10px";>Week 14 (15 - 21 September)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="title4">Communication and financial support:</p></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 641:</td>
<td colspan="2" class="diff-lineno">Line 641:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title4</del>">Weekly meeting with the instructors(09/18/2014)</p> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title3</ins>">Weekly meeting with the instructors(09/18/2014)</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).</div></td></tr>
</table>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=349917&oldid=prevManon at 20:20, 17 October 20142014-10-17T20:20:57Z<p></p>
<a href="http://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=349917&oldid=349626">Show changes</a>Manonhttp://2014.igem.org/wiki/index.php?title=Team:Toulouse/Notebook/Calendar&diff=349626&oldid=prevManon at 20:18, 17 October 20142014-10-17T20:18:03Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:18, 17 October 2014</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Communication and financial support:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Increase in our communication strategy to find more sponsors specialized in environment and ecological matters <br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Increase in our communication strategy to find more sponsors specialized in environment and ecological matters <br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 2 (23-29 June)</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 2 (23-29 June)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Communication and financial support:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- BioSynSyS conference work: powerpoint, logo in order to present our SubtiTree project to a whole audience of scientific and researchers</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- BioSynSyS conference work: powerpoint, logo in order to present our SubtiTree project to a whole audience of scientific and researchers</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Lab work:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- E. coli transformation with BBA_J004450 (pSB1C3)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- E. coli transformation with BBA_J004450 (pSB1C3)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Weekly meeting with the instructors (06/27/2014)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Weekly meeting with the instructors (06/27/2014)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Concentration of antibiotics in media must be checked<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Concentration of antibiotics in media must be checked<br/></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 3 (30 June -6 July) and Week 4 (7-13 July)</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 3 (30 June -6 July) and Week 4 (7-13 July)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Communication and financial support:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Communication and financial support:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Participation at the BioSynSys conferences: presentation of SubtiTree<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Participation at the BioSynSys conferences: presentation of SubtiTree<br/></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 282:</td>
<td colspan="2" class="diff-lineno">Line 282:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Lab work:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 290:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Weekly meetings with the instructors (07/04/2014) and (07/11/2014)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Weekly meetings with the instructors (07/04/2014) and (07/11/2014)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- iGEM efficiency kit does not work, we shall not use it anymore<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- iGEM efficiency kit does not work, we shall not use it anymore<br/></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 303:</td>
<td colspan="2" class="diff-lineno">Line 303:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 5 (14-20 July)</p></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="technology2"><p class="title3"; "line-height:6px"; "padding:10px";>Week 5 (14-20 July)</p></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="thelanguage2"></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class="title4">Communication and financial support:</p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Our team was interviewed by many local newspapers such as 20minutes, Metronews, La Voix du Midi, La Dépêche but also by the local television channel France3 Midi-Pyrénées to present our draft to the community. Moreover, SubtiTree was approached in some radio programs like Virgin Radio Toulouse and France info.</br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Our team was interviewed by many local newspapers such as 20minutes, Metronews, La Voix du Midi, La Dépêche but also by the local television channel France3 Midi-Pyrénées to present our draft to the community. Moreover, SubtiTree was approached in some radio programs like Virgin Radio Toulouse and France info.</br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Support of ThermoFisher, New Englands BioLabs, Qiagen, Eurofins but also GenoToul, Labex Tulip, L’Université Paul Sabatier, CROUS.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Support of ThermoFisher, New Englands BioLabs, Qiagen, Eurofins but also GenoToul, Labex Tulip, L’Université Paul Sabatier, CROUS.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Lab work:</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Lab work:</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- GFP and RFP digestion amplified by miniprep and gel electrophoresis <br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- GFP and RFP digestion amplified by miniprep and gel electrophoresis <br/></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 317:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p class="<del class="diffchange diffchange-inline">title3</del>">Weekly meeting with the instructors (07/18/2014)</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p class="<ins class="diffchange diffchange-inline">title4</ins>">Weekly meeting with the instructors (07/18/2014)</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p class="texte"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Eurofins genes<br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- Transformation of the Eurofins genes<br/></div></td></tr>
</table>Manon