Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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- iGEM competition presentation and explanations<br/>
- iGEM competition presentation and explanations<br/>
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- Constitution of the team by interviewing different students from Université Paul Sabatier and INSA
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- Constitution of the <a href="https://2014.igem.org/Team:Toulouse/Team">team</a> by interviewing different students from Université Toulouse III Paul Sabatier and INSA de Toulouse
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<p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team ! </B>
<p class="texte" style="text-align:center"><B> The adventure begins for the 2014 Toulouse iGEM Team ! </B>
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- Thanks to weekly meetings, our team was able to discuss about new project ideas.
- Thanks to weekly meetings, our team was able to discuss about new project ideas.
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- Many ideas were approached and debated regarding the feasibility thanks to the literature and supervisors including teachers and researchers
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- Many ideas were approached and debated regarding the feasibility thanks to the litterature and supervisors including teachers and researchers.<br>
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This is a list of the main projects:  
This is a list of the main projects:  
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<div class="technology"><p class="title2"; "line-height:6px"; "padding:10px";>June 2014: Choice of SubtiTree project</p></div>
<div class="technology"><p class="title2"; "line-height:6px"; "padding:10px";>June 2014: Choice of SubtiTree project</p></div>
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<p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of June, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br>
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<p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of May, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br>
Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br>
Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br>
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By this period, we evaluated our budget to approximately <B> k€ 40,000 </B>.
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By this period, we evaluated our budget to approximately <B> €39,500 </B>.
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- Beginning of the laboratory work to create our optimized bacterium <br/>
- Beginning of the laboratory work to create our optimized bacterium <br/>
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- Research of sponsors and the communication thanks to the press
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- Research of sponsors and communication thanks to the press
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<p class="title4">Communication and financial support:</p>
<p class="title4">Communication and financial support:</p>
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- Participation at the BioSynSys conferences: presentation of SubtiTree<br/>
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- Participation at the BioSynSys conferences: presentation of SubtiTree project<br/>
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- We have put in place a newsletter system the first day of each month to keep all our sponsors and people who support us updated about the project, our financial state.
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- We have put in place a newsletter system the first friday of each month to keep all our sponsors and people who support us updated about the project (financial, scientific and communication aspects).
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- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br>
- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br>
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Problem: A problem was reported in the use of the CYP. Indeed, the biobrick is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/>
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Problem: A problem was reported in the use of the CYP. Indeed, the BioBrick is not expressed properly in <i>Bacillus subtilis</i> strain. Therefore we had to look at the literature to find a new fungicide called GAFP-1.<br/>
- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of <i>Bacillus</i> strain in a medium composed of many different carbon sources.<br/>
- A bioreactor was conceived to reproduce the composition of the sap in the plane trees. The main goal was to identify the behavior of <i>Bacillus</i> strain in a medium composed of many different carbon sources.<br/>
- Competent cells and transformation practice using GFP and RFP.
- Competent cells and transformation practice using GFP and RFP.

Revision as of 20:33, 17 October 2014