Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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<p class="title2">Lab work:</p>
<p class="title2">Lab work:</p>
<p class="texte">
<p class="texte">
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- Evaluation of the bacterial decrease rate from 10°C to 4°C with a sporuling strain of Bacillus subtilis.
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- Evaluation of the bacterial decrease rate from 10°C to 4°C with a sporuling strain of <i>Bacillus subtilis</i>.
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- Chemotaxis test with different concentration of glucose on a 0.3% agar.
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- Chemotaxis test with different concentrations of glucose on a 0.3% agar.
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- Miniprep of pSBBs4S with binding gene and transformation in <i>Bacillus subtilis</i>.
- Miniprep of pSBBs4S with binding gene and transformation in <i>Bacillus subtilis</i>.
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<p class="title3">Lab work:</p>
<p class="title3">Lab work:</p>
<p class="texte">
<p class="texte">
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- Subculture and transformation in Bacillus subtilis of BBa_K1364011 in pSBBS4S (BBa_K823022) and cloning of BBa_K1364011 on pSBBS1C (BBa_K823023).
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- Subculture and transformation in <i> Bacillus sutbilis </i> of BBa_K1364011 in pSBBS4S (BBa_K823022) and cloning of BBa_K1364011 on pSBBS1C (BBa_K823023).
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Problem: no clone had the insert on pSBBS1C => the cloning had to be done again
Problem: no clone had the insert on pSBBS1C => the cloning had to be done again

Revision as of 14:38, 16 October 2014