Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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- Discussion about EcAMP cloning which presents some issues
- Discussion about EcAMP cloning which presents some issues
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<br/>
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- Discussion about the transfer of pSB1C3 from E. coli to Bacillus strain
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- Discussion about the transfer of pSB1C3 from <i>E. coli</i> to <i>Bacillus</i> strain
</p>  
</p>  
   
   
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- Elaboration of an efficient fungicide test protocol  
- Elaboration of an efficient fungicide test protocol  
<br/>
<br/>
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- Test of the fungus growth on PDA medium + test of growth for Bacillus subtilis liquid culture on PDA
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- Test of the fungus growth on PDA medium + test of growth for <i>Bacillus subtilis</i> liquid culture on PDA
</p>
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- Ask iGEM headquarters if all the biobricks must be on pSB1C3 plasmid
- Ask iGEM headquarters if all the biobricks must be on pSB1C3 plasmid
<br/>
<br/>
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- Use another strain of E. coli (DH5-1 instead of DH5 alpha) to have a faster growth
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- Use another strain of i>E. coli</i> (DH5-1 instead of DH5 alpha) to have a faster growth
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<br/>
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- Check which quantity of Bacillus subtilis is necessary to naturally destroy the fungus
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- Check which quantity of <i>Bacillus subtilis</i> is necessary to naturally destroy the fungus
</p>
</p>
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- Cloning of BBa_K1364014 in BBa_K823022 and in BBa_K823023  
- Cloning of BBa_K1364014 in BBa_K823022 and in BBa_K823023  
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- Cloning GAFP1 + BBa_K823023 (pSBBS1C) in Bacillus subtilis with a change in the protocol: pre-induction of the culture with 0.125 µg/ml of chloramphenicol one hour after the addition of DNA, and let grow for another hour.
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- Cloning GAFP1 + BBa_K823023 (pSBBS1C) in <i>Bacillus subtilis</i> with a change in the protocol: pre-induction of the culture with 0.125 µg/ml of chloramphenicol one hour after the addition of DNA, and let grow for another hour.
<br/>
<br/>
Problem: no colony  
Problem: no colony  
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- Liquid culture of Bacillus + BBA_1364002 (GAFP1)  ;  Bacillus + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1)  ;  Bacillus + BBA_1364003 (D4E1)  for future fungicide tests
- Liquid culture of Bacillus + BBA_1364002 (GAFP1)  ;  Bacillus + BBA_1364003 (D4E1) + BBA_1364002 (GAFP1)  ;  Bacillus + BBA_1364003 (D4E1)  for future fungicide tests
<br/>
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- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSBBs1C) and transformation in Bacillus subtilis strain.  
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- Miniprep of BBa_ K1364011 in BBa_ K823023 (pSBBs1C) and transformation in <i>Bacillus subtilis</i> strain.  
<br/>
<br/>
- Fungicide test of BBa_K1364011 in pSBBs4S,  Promotor  + BBA_1364002 (GAFP1)  + Terminator (biobrick BBa_K1364008) in pSBBS4S,  Promotor  + BBA_1364003 (D4E1)  + Terminator  (biobrick BBa_K1364009) in pSBBS4S, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1)  + Terminator ( biobrick BBa_K1364013) in pSBBS4S
- Fungicide test of BBa_K1364011 in pSBBs4S,  Promotor  + BBA_1364002 (GAFP1)  + Terminator (biobrick BBa_K1364008) in pSBBS4S,  Promotor  + BBA_1364003 (D4E1)  + Terminator  (biobrick BBa_K1364009) in pSBBS4S, Promotor + BBA_1364002 (GAFP1) + BA_1364003 (D4E1)  + Terminator ( biobrick BBa_K1364013) in pSBBS4S
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- The first sequencing was not successful => new sequencing with one primer/tube<br/>
- The first sequencing was not successful => new sequencing with one primer/tube<br/>
- Chemotaxis test: increase the concentration in bacterium, try a new protocol by capillarity<br/>
- Chemotaxis test: increase the concentration in bacterium, try a new protocol by capillarity<br/>
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- End of the binding test: IT WORKS PERFECTLY! SubtiTree has the good phenotype and presents a better binding property than the wild type Bacillus subtilis strain.<br/>
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- End of the binding test: IT WORKS PERFECTLY! SubtiTree has the good phenotype and presents a better binding property than the wild type <i>Bacillus subtilis</i> strain.<br/>
- Fungicides test: contaminants with the fungus. Need to ask for another culture of our fungi.
- Fungicides test: contaminants with the fungus. Need to ask for another culture of our fungi.
</p>
</p>
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- Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
- Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
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<br/>- Beginning of the organization of the iGEM Toulouse 2014 acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
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<br/>- Beginning of the organization of the Toulouse iGEM team acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
<br/>- Final design of the wiki
<br/>- Final design of the wiki
</p>
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<p class="texte">
<p class="texte">
- Focus on the wiki design and writing.
- Focus on the wiki design and writing.
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<br/>- Start making the power point presentation for Boston and the poster.
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<br/>- Start making the power point presentation for the Giant Jamboree and the poster.
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<br/>- Division of responsibilities for all the non-scientific work.
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<br/>- Division of responsibilities for the non-scientific work.
</p>
</p>

Revision as of 14:36, 16 October 2014