Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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<br/> Try different temperatures for the fungicide tests: 20°C, 25°C, and 37°C to reduce the fungus growth.
<br/> Try different temperatures for the fungicide tests: 20°C, 25°C, and 37°C to reduce the fungus growth.
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<p>
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<div class="Sub_title"> Week 14 (15 - 21 September) <a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>
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<br/> </FONT>
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<p>
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<B> Communication and financial support: </B>
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<p>
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<br/>-      Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
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<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
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<br/>- Beginning of the organization of the iGEM Toulouse 2014 acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
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<br/>- Final design of the wiki
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<p>
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<B> Lab work: </B>
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<br/>- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1-D4E1)
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<br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)
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<br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)
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<br/>- Chemotaxis test with wild type Bacillus subtilis strain using Imperial College protocol
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<br/>- Tansformation of BBa_K1364011 + BBa_K823022 (pSBBS4S) in Bacillus subtilis
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<br/>- Culture of fungi with fungicides
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<br/>- Sequencing of plasmids containing the fungicides
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<CENTER> <I> Weekly meeting with the instructors(09/18/2014)</I> </CENTER>
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<br/> Sequencing worked for D4E1 and GAFP1 and the mini operon BBa_K1364013 (GAFP1-D4E1).
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<br/> The next step is to sequence the maxi operon (EcAMP1 – GAFP1 – D4E1).
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<br/> Try different approaches for the chemotaxis:
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<br/>- New protocol with the capillary assay with a new system made by a glassblower.
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<br/>- Make the Imperial College test again by testing 3 different solutions: chitine, N-acetylglucosamine and another carbon source.
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<br/> Decrease the number of conidia and temperature for the fungicide test.
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<p>
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<div class="Sub_title"> Week 15 (22 - 28 September) <a href="http://2014.igem.org/Team:Toulouse/Project/Chemotaxis" class ="Link">Show more</a></div>
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<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>
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<br/> </FONT>
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<p>
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<B> Communication and financial support: </B>
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<p>
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<br/>- New support of Sallèles d’Aude city hall.
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<br/>- Payment of the plane tickets.
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<br/>- Acknowledgement day (4th December): contact with catering service, reservation of the amphitheater, list of guests…
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<br/>- Order of the T shirt for the Jamboree! We look awesome with them 
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<p>
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<B> Lab work: </B>
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<br/>- Last experiments regarding chemotaxis tests.
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<CENTER> <I> Weekly meeting with the instructors(09/26/2014)</I> </CENTER>
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<br/> Focus on the wiki design and writing.
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<br/> Start making the power point presentation for Boston and the poster.
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<br/> Division of responsibilities for all the non-scientific work.
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Revision as of 13:01, 1 October 2014