Team:Toulouse/Notebook/Calendar

From 2014.igem.org

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<div class="technology">December 2013 – January 2014: Team selection</div>
<div class="technology">December 2013 – January 2014: Team selection</div>
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- iGEM competition presentation and explanations<br/>
- iGEM competition presentation and explanations<br/>
- Constitution of the team by interviewing different students from Université Paul Sabatier and INSA<br/>
- Constitution of the team by interviewing different students from Université Paul Sabatier and INSA<br/>
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<div class="technology">January – June 2014: Projects brainstorming</div>
<div class="technology">January – June 2014: Projects brainstorming</div>
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- Thanks to weekly meetings, our team was able to discuss about new project ideas and publications.
- Thanks to weekly meetings, our team was able to discuss about new project ideas and publications.
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<div class="technology">June 2014: Choice of SubtiTree project</div>
<div class="technology">June 2014: Choice of SubtiTree project</div>
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<p class="texte">The discussion has been long to estimate the practicabilty of our final project. By the end of June, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br>
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<p class="texte">The discussion has been long to estimate the practicability of our final project. By the end of June, most of the ideas were eliminated but two of them remained: controlling the cancer or saving the trees. It became easily clear that SubtiTree was successfully completed whether regarding the scientific part or the ethical aspect. Indeed, our knowledge in medicine was not sufficient enough to evaluate the consequences of a bacterium injection in the human body for <i>E. coli</i> cancer.</br>
Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br>
Therefore, the whole team agreed on focusing on a local issue as the preservation of the Canal du Midi plane trees. However, the final goal was based on the idea to allow our optimized bacteria to be efficient for all fungi infections in the environment.</br>
By this period, we evaluated our budget to approximately <B> 40 k€ </B>.
By this period, we evaluated our budget to approximately <B> 40 k€ </B>.
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<div class="technology">Week 1(16-22 June)</div>
<div class="technology">Week 1(16-22 June)</div>
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<div class="technology">Week 3 (30 June -6 July) and Week 4 (7-13 July)</div>
<div class="technology">Week 3 (30 June -6 July) and Week 4 (7-13 July)</div>
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<p class="title3">Lab work: </p>
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<p class="title3">Lab work:</p>
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- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br>
- Design of all the constructions needed with cloning and restriction enzymes to save some time and allow a better comprehension of our bacterium system </br>
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<div class="technology">Week 5 (14-20 July)</div>
<div class="technology">Week 5 (14-20 July)</div>
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<div class="technology">Week 6 (21 July-27 July)</div>
<div class="technology">Week 6 (21 July-27 July)</div>
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<div class="technology">August 2014 </div>
<div class="technology">August 2014 </div>
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<div class="technology">Week 7 (28 July-3 August)</div>
<div class="technology">Week 7 (28 July-3 August)</div>
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<div class="technology">Week 8 (04-10 August)</div>
<div class="technology">Week 8 (04-10 August)</div>
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- Check which quantity of Bacillus subtilis is necessary to naturally destroy the fungus
- Check which quantity of Bacillus subtilis is necessary to naturally destroy the fungus
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<div class="technology">Week 9 (11-17 August)</div>
<div class="technology">Week 9 (11-17 August)</div>
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- Miniprep of pSBbs4S with binding gene and transformation in <i>Bacillus subtilis</i>.
- Miniprep of pSBbs4S with binding gene and transformation in <i>Bacillus subtilis</i>.
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Problem: no digestion was visible on the gel the cloning failed
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Problem: no digestion was visible on the gel => the cloning failed
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- Transformation of BBa_K1374010 (Pveg + RBS SpoVG + EcAMP) + BBa_K1364012 (RBS + Gafp1 + D4E1 + double terminator), subculture
- Transformation of BBa_K1374010 (Pveg + RBS SpoVG + EcAMP) + BBa_K1364012 (RBS + Gafp1 + D4E1 + double terminator), subculture
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- Fungicide test
- Fungicide test
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<div class="technology">Week 10 (18-24 August)</div>
<div class="technology">Week 10 (18-24 August)</div>
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<p class="title3">Communication and financial support:</p>
<p class="title3">Communication and financial support:</p>
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<div class="technology">Week 11 (25- 31 August)</div>
<div class="technology">Week 11 (25- 31 August)</div>
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<div class="technology">Week 12 (1-7 September)</div>
<div class="technology">Week 12 (1-7 September)</div>
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<div class="technology">Week 13 (8- 14 September)</div>
<div class="technology">Week 13 (8- 14 September)</div>
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<div class="technology">Week 14 (15 - 21 September)</div>
<div class="technology">Week 14 (15 - 21 September)</div>
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<div class="technology">Week 15 (22 - 28 September)</div>
<div class="technology">Week 15 (22 - 28 September)</div>
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<div class="technology">October 2014</div>
<div class="technology">October 2014</div>

Revision as of 22:05, 15 October 2014