Team:Toulouse/Notebook/Calendar

From 2014.igem.org

(Difference between revisions)
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<p class="title1">July 2014</p>
<p class="title1">July 2014</p>
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<p class="textesimple">Main activities</p>
+
<p class="title2">Main activities</p>
<p class="texte">
<p class="texte">
- Beginning of the laboratory work to create our optimized bacterium <br/>
- Beginning of the laboratory work to create our optimized bacterium <br/>
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<p class="title1">August 2014 </p>
<p class="title1">August 2014 </p>
-
<p class="textesimple">Main activites</p>
+
<p class="title2">Main activities</p>
<p class="texte">
<p class="texte">
- Finishing the cloning for the different parts of the bacterium<br/>
- Finishing the cloning for the different parts of the bacterium<br/>
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</p>
</p>
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<p class="title3">Lab work: </p>
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<p class="title3">Lab work:</p>
<p class="texte">
<p class="texte">
- Subculture and transformation in Bacillus subtilis of BBa_K1364011 in pSBBS4S (BBa_K823022) and cloning of BBa_K1364011 on pSBBS1C (BBa_K823023).
- Subculture and transformation in Bacillus subtilis of BBa_K1364011 in pSBBS4S (BBa_K823022) and cloning of BBa_K1364011 on pSBBS1C (BBa_K823023).
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<p class="title1"> September 2014</p>
<p class="title1"> September 2014</p>
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<p class="textesimple">Main activites</p>
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<p class="title2">Main activities</p>
<p class="texte">
<p class="texte">
- Finish the tests of the different modules
- Finish the tests of the different modules
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<p class="title3">Weekly meeting with the instructors(09/05/2014)</p>  
<p class="title3">Weekly meeting with the instructors(09/05/2014)</p>  
<p class="texte">
<p class="texte">
-
- The first sequencing was not successful new sequencing with one primer/tube<br/>
+
- The first sequencing was not successful => new sequencing with one primer/tube<br/>
- Chemotaxis test: increase the concentration in bacterium, try a new protocol by capillarity<br/>
- Chemotaxis test: increase the concentration in bacterium, try a new protocol by capillarity<br/>
- End of the binding test: IT WORKS PERFECTLY! SubtiTree has the good phenotype and presents a better binding property than the wild type Bacillus subtilis strain.<br/>
- End of the binding test: IT WORKS PERFECTLY! SubtiTree has the good phenotype and presents a better binding property than the wild type Bacillus subtilis strain.<br/>
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</p>
</p>
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<CENTER> <I> Weekly meeting with the instructors(09/11/2014)</I> </CENTER>  
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<p class="title3">Weekly meeting with the instructors(09/11/2014)</p>  
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<br/>
+
<p class="texte">
-
<br/> Try to use the GFP or RFP to follow the chemotaxis test.
+
- Try to use the GFP or RFP to follow the chemotaxis test.
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<br/>Make a new glass process for the chemotaxis capillary test.
+
<br/>- Make a new glass process for the chemotaxis capillary test.
-
<br/>Possibility of contacting an INRA laboratory to get pictures of the binding test with confocal microscope.
+
<br/>- Possibility of contacting an INRA laboratory to get pictures of the binding test with confocal microscope.
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<br/>Sequencing the binding construction.
+
<br/>- Sequencing the binding construction.
-
<br/>Try different temperatures for the fungicide tests: 20°C, 25°C, and 37°C to reduce the fungus growth.
+
<br/>- Try different temperatures for the fungicide tests: 20°C, 25°C, and 37°C to reduce the fungus growth.
 +
</p>
-
<p>
+
<p class="title2">Week 14 (15 - 21 September)</p>
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<br/>
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<p class="title3">Communication and financial support:</p>
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<FONT SIZE= 2%> 
+
<p class="texte">
-
<div class="Sub_title"> Week 14 (15 - 21 September)</div>
+
- Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
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<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>
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<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
-
<br/> </FONT>
+
<br/>- Beginning of the organization of the iGEM Toulouse 2014 acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
-
<p>
+
<br/>- Final design of the wiki
-
<B> Communication and financial support: </B>
+
</p>
-
<br/>
+
-
<p>
+
-
<br/>-       Support of two French city halls: Mairie de Montréal, Mairie de Ventenac en Minervois.
+
-
<br/>- First interview by the French national radio France Inter which will be aired on October 12th.
+
-
<br/>- Beginning of the organization of the iGEM Toulouse 2014 acknowledgement day: the purpose is to present the project to the students and give a feedback on the competition to the sponsors.
+
-
<br/>- Final design of the wiki
+
-
<br/>
+
-
<p>
+
-
<B> Lab work: </B>
+
-
<br/>
+
-
<br/>- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1-D4E1)
+
-
<br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)
+
-
<br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)
+
-
<br/>- Chemotaxis test with wild type Bacillus subtilis strain using Imperial College protocol
+
-
<br/>- Tansformation of BBa_K1364011 + BBa_K823022 (pSBBS4S) in Bacillus subtilis
+
-
<br/>- Culture of fungi with fungicides
+
-
<br/>- Sequencing of plasmids containing the fungicides
+
 +
<p class="title3">Lab work:</p>
 +
<p class="texte">
 +
- Threonine tests for the BBa_K1364014 maxi operon (EcAMP1 – GAFP1 – D4E1) and mini operon BBa_K1364013 (GAFP1-D4E1)
 +
<br/>- Colony PCR on the mini operon BBa_K1364013 (GAFP1 - D4E1)
 +
<br/>- Colony PCR on BBa_K1364011 on BBa_K823022 (pSBBS4S)
 +
<br/>- Chemotaxis test with wild type <i>Bacillus subtilis</i> strain using Imperial College protocol
 +
<br/>- Tansformation of BBa_K1364011 + BBa_K823022 (pSBBS4S) in Bacillus subtilis
 +
<br/>- Culture of fungi with fungicides
 +
<br/>- Sequencing of plasmids containing the fungicides
 +
</p>
<CENTER> <I> Weekly meeting with the instructors(09/18/2014)</I> </CENTER>  
<CENTER> <I> Weekly meeting with the instructors(09/18/2014)</I> </CENTER>  
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<br/> Decrease the number of conidia and temperature for the fungicide test.
<br/> Decrease the number of conidia and temperature for the fungicide test.
-
<p>
+
<p class="title2">Week 15 (22 - 28 September)</p>
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<br/>
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<p class="title3">Communication and financial support:</p>
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<FONT SIZE= 2%> 
+
<p class="texte">
-
<div class="Sub_title"> Week 15 (22 - 28 September)</div>
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- New support of Sallèles d’Aude city hall.
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<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>
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<br/>- Payment of the plane tickets.
-
<br/> </FONT>
+
<br/>- Acknowledgement day (4th December): contact with catering service, reservation of the amphitheater, list of guests…
-
<p>
+
<br/>- Order of the T shirt for the Jamboree! We look awesome with them!
-
<B> Communication and financial support: </B>
+
</p>
-
<br/>
+
-
<p>
+
-
<br/>- New support of Sallèles d’Aude city hall.
+
-
<br/>- Payment of the plane tickets.
+
-
<br/>- Acknowledgement day (4th December): contact with catering service, reservation of the amphitheater, list of guests…
+
-
<br/>- Order of the T shirt for the Jamboree! We look awesome with them
+
-
+
-
<br/>
+
-
<p>
+
-
<B> Lab work: </B>
+
-
<br/>
+
-
<br/>- Last experiments regarding chemotaxis tests.
+
 +
<p class="title3">Lab work: </p>
 +
<p class="texte">
 +
- Last experiments regarding chemotaxis tests.
 +
</p>
-
<CENTER> <I> Weekly meeting with the instructors(09/26/2014)</I> </CENTER>
 
-
<br/>
 
-
<br/> Focus on the wiki design and writing.
 
-
<br/> Start making the power point presentation for Boston and the poster.
 
-
<br/> Division of responsibilities for all the non-scientific work.
 
-
<br>
+
<p class="title3">Weekly meeting with the instructors(09/26/2014)</p>
-
<p>
+
<p class="texte">
 +
- Focus on the wiki design and writing.
 +
<br/>- Start making the power point presentation for Boston and the poster.
 +
<br/>- Division of responsibilities for all the non-scientific work.
 +
</p>
 +
 
<div class="Sub_title"> October 2014 </div>
<div class="Sub_title"> October 2014 </div>
<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>
<table width="50%"><tr><td bgColor="#097F09" height="1"></td> </tr></table>

Revision as of 10:42, 9 October 2014