Team:Toulouse/Modelling

From 2014.igem.org

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Modelling is a tool used to simplify and study systems. We can try to predict behavior with bibliographic information or information obtained from experiment.</br>
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Modelling is a tool used to simplify and study systems. We can try to predict behavior with bibliographic informations or informations obtained from experiment.</br>
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Our project focuses on the development of our engineered bacteria in tree. The bacterial growth in tree seems to be unknown, so we must infer <i>Bacillus subtilis</i> behavior.</p>
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Our project focuses on the development of our engineered bacterium in tree. The bacterial growth in trees seems to be unknown, so we must infer <i>Bacillus subtilis</i> behavior.</p>
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<i>Bacillus subtilis</i> is a trees endophyte strain. A study <b>[1]</b> showed that <i>Bacillus subtilis</i> could develop and fully colonize a tree, reaching a concentration of 10⁵ cells per gram of fresh plant. We need to know in which conditions the growth of <i>B. subtilis</i> is optimum in a tree and if the weather can stop its development during winter. So we decided to work on the <i>Bacillus subtilis</i> growth in function of the temperature during the year. Modeling bacterial growth in a tree section generates some difficulties, we need to know distance between two tree extremities (treetops and root) or the speed sap flow which can vary with temperatures during the day and seasons, cause of the type of sap (phloem, xylem). Furthermore a tree is not an homogeneous system, its roots, trunk and branch do not contain same amount of sap and wood. The average speed of the plane tree sap is 2.4m/h <b>[2]</b>, which means that in a day the sap will flow from one end to the other of a tree 30m. Tree is reduced to a bioreactor.
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<i>Bacillus subtilis</i> is a tree endophyte strain. A study <b>[1]</b> showed that <i>Bacillus subtilis</i> could develop and fully colonize a tree, reaching a concentration of 10⁵ cells per gram of fresh plant. We need to know in which conditions the growth of <i>B. subtilis</i> is optimum in a tree and if the weather can stop its development during winter. So we decided to work on the <i>Bacillus subtilis</i> growth in function of the temperature during the year. Modelling bacterial growth in a tree section generates some difficulties, we need to know the distance between two tree extremities (treetops and root) or the speed sap flow which can vary with temperatures during the day and seasons, cause of the type of sap (phloem, xylem). Furthermore a tree is not an homogeneous system, its roots, trunk and branch do not contain same amount of sap and wood. The average speed of the plane tree sap is 2.4m/h <b>[2]</b>, which means that in a day the sap will flow from one end to the other of a tree 30m. Tree is reduced to a bioreactor.
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Revision as of 19:34, 13 October 2014