Team:TU Eindhoven/Modeling/Bacterial Cell Counter

From 2014.igem.org

Revision as of 10:22, 11 October 2014 by Rafiqlubken (Talk | contribs)

iGEM Team TU Eindhoven 2014

iGEM Team TU Eindhoven 2014

Bacterial Cell Counter

When photos of the microfluidics results are taken, the droplets and the cells in the droplets have to be counted. In order to have reliable results this has to be done on a large scale. To make it possible to analyses large numbers of photos a computer program has been made to do the counting. To give a better visualization of the process, a step by step analysis of an image is given below.

Figuur 1. Sample image used to show how the program works.

Figure 1. Poisson distribution for multiple lambdas.

This allows for the determination of the ratios between one cell/zero cells and one cell/ two or more cells. These both have to be as high as possible for a specific lambda.

Figure 2. Ratio between one cell/ zero cells or more cells

Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.

Figure 3.

Bibliography

iGEM Team TU Eindhoven 2014