Team:TU Delft-Leiden/Project/Life science/curli/cloning/GoldenGate

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Curli Module - Cloning - Golden Gate Assembly

Figure 1. Golden Gate Assembly of BBa_K1316013 and BBa_K1316014 . BBa_K1316013 and BBa_K1316014 are made up of a rhamnose promoter, csgB and csgA or csgA-HIS. The BioBrick encodes a rhamnose promoter (red rectangle) and the coding sequences of csgB and csgA, indicated with grey arrows. The beam beneath the arrows visualizes the regions and relative sizes of the 3 DNA pieces that were ligated into pSB1C3.
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