Team:TU Delft-Leiden/Project/Life science/curli

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    Module Conductive Curli
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<h2> Curli Module</h2>
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        Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.
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<h3> Background Information</h3>
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            Module Conductive Curli
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/theory">Context</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/integration">Integration of Departments</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/cloning">Cloning</a></li>
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            <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation">Characterization</a></li>
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In de module CONDUCTIVE CURLI we combine the advantages of live bacteria with the benefits of nonliving materials. A great advantage of bacteria is their ability to respond to the environment, but unfortunately, they have limited possibility to create new functionality all of the sudden. However, if you would find a way to combine bacteria with nonliving materials, you can choose your material in a way it meets your requirements. A beautiful example of these “living materials” has recently been shown by MIT engineers, who were able to reprogram <i>E. coli</i> in a way in which the bacteria were actually making gold nanowires and conducting biofilms when gold nanoparticles were present [1].
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Keen to see our <b>conclusions</b> for this module?  See the list below!
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<li>The constructs made are capable of generating curli-forming proteins and biofilm as proved by Congo red and crystal violet assays respectively.</li>
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For the CONDUCTIVE CURLI part of the project we induce curli formation, which are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation [2]. The curli pathway involves the so called CsgA-G proteins (see figure 1). CsgA is the major curli subunit, with a CsgB subunit at the beginning of every fiber. CsgE, CsgF and CsgG are non-structural proteins involved in curli biogenesis and CsgD is a transcriptional regulator [3].
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<li>Curli formation happens in response to induction of the used promoter.</li></ul></p>
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<p> Want to know how we came to these conclusions? Go to our <a href= https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation><b>Characterization</b></a> page!
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Figure 1. <b>curli</b>. xxx. Source image: adapted from M. M. Barnhart and M. R. Chapman (2006).
 
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<p> Our curli carry histidine tags, which facilitates binding of the gold nanoparticles to the curli [1]. The gold nanoparticles on their turn end up in the biofilm, improving the conductivity of the extracellular environment. This change in conductivity is easily measurable by our ELECTRACE device, but in combination with the <a href="https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/EET">extracellular electron transport module</a> , it could even result in enhanced extracellular electron transport.
 
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<h2> Cloning Strategy and Characterisation of this module</h2>
 
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  <li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/cloning"><p>Cloning</p>
 
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<li><a href="/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation"><p>Characterisation</p>
 
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<h3> References </h3>
 
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1. Chen et al., Synthesis and patterning of tunable multiscale materials with engineered cells. <i>Nature Materials</i> 13, 515–523 (2014)
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Interested in one of our other Modules? Navigate to the <a href=" https://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/EET"> <b> Module Electron Transport </b> </a> where you can find everything regarding the implementation of the MtrCAB conduit. Go to our <a href="https://2014.igem.org/Team:TU_Delft-Leiden/WetLab/landmine"> <b> Module Landmine Detection </b> </a> page and discover how we tweaked <i> E. coli</i> to let it respond on landmines.
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2. M. M. Barnhart and M. R. Chapman, Curli Biogenesis and Function.  <i>Annu Rev Microbiol.</i> 60, 131–147 (2006)
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3. L.S. Robinson et al., Secretion of curli fibre subunits is mediated by the outer membrane-localized CsgG protein.<i> Mol Microbiol. </i>59(3): 870–881. (2006)
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        <img title="Curli" src="https://static.igem.org/mediawiki/2014/3/3c/Curli.jpg" style="display:block;margin-left:auto;margin-right:auto;height:50%;width:50%;" />
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Latest revision as of 20:53, 17 October 2014

Module Conductive Curli

Information with respect to literature consulted regarding the Modules is referred to under Context. Also, each of the three complementary Modules is equipped with an Integration of Departments, in which it is described how the Departments Modeling, Experimental Work and Microfluidics interact. Furthermore, each Module contains information on Cloning and results are presented under Characterization.

Keen to see our conclusions for this module? See the list below!

  • The constructs made are capable of generating curli-forming proteins and biofilm as proved by Congo red and crystal violet assays respectively.
  • Curli formation happens in response to induction of the used promoter.

Want to know how we came to these conclusions? Go to our Characterization page!


Interested in one of our other Modules? Navigate to the Module Electron Transport where you can find everything regarding the implementation of the MtrCAB conduit. Go to our Module Landmine Detection page and discover how we tweaked E. coli to let it respond on landmines.

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