Team:TCU Taiwan/SystemDesign
From 2014.igem.org
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- | <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/4/ | + | <td width="128" height="128" align="left" valign="middle"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128" height="128"></td> |
- | <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644"> | + | <td width="68%" align="left" valign="middle"><h6><font face="Trebuchet MS" size="7" color="#484644">System Design</font></h6></td> |
<td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td> | <td width="19%" align="center"><img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="90%"></td> | ||
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<li><a href="#" style="cursor:default"><font size="5" style="color:#DBAC52">Project</font></a> | <li><a href="#" style="cursor:default"><font size="5" style="color:#DBAC52">Project</font></a> | ||
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- | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project | + | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Project"><font size="3">Introduction</font></a></li> |
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><font size="3">Parts</font></a></li> | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><font size="3">Parts</font></a></li> | ||
- | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign"><font size="3">System Design</font></a></li> | + | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign" style="color:#DBAC52"><font size="3">System Design</font></a></li> |
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/M13Phage"><font size="3">M13 Phage</font></a></li> | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/M13Phage"><font size="3">M13 Phage</font></a></li> | ||
- | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/CRISPR | + | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/CRISPR"><font size="3">CRISPR</font></a></li> |
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/FutureWorks"><font size="3">Future Works</font></a></li> | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/FutureWorks"><font size="3">Future Works</font></a></li> | ||
<li><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><font size="3">Human Pratics</font></a></li> | <li><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><font size="3">Human Pratics</font></a></li> | ||
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+ | <td colspan="3" style="background-color:#FFF2B5" height="20"><font face="Trebuchet MS" size="6" color="#A66B38">CRISPR Construction</font></td> | ||
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+ | <td width="50%"><table width="90%" border="1" cellspacing="0" cellpadding="0" align="center"> | ||
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+ | <td><img src="https://static.igem.org/mediawiki/2014/d/d8/TCU_SD10733136_390371157783708_1318933891_o.jpg" width="100%"/></td> | ||
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+ | <td align="center"><font size="3" face="Verdana"><strong>Fig.1</strong></font></td> | ||
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+ | <td><font size="3" face="Verdana" color="#333"><p>We synthesised a new CIRSPR system based on a CRISPR designed by Standford-Brown 2013 (<a href="http://parts.igem.org/Part:BBa_K1218011" target="_blank">BBa_K1218011</a>). Here we analyzed its sequence and then removed its Cas9 protein with Cas9’s promoter because we want our Cas9’s expression can be inducible. So then we synthesized a new Cas9 structure which contains an inducible promoter, pRha (<a href="http://parts.igem.org/Part:BBa_K914003" target="_blank">BBa_K914003</a>). This promoter can be induced by rhamnose and silenced by glucose, both sugar can be find in nature. Next, we put our inducible Cas9 back to the CRISPR system, and because tracrRNA and crRNA have no function without Cas9 protein, this CRISPR is now under our control.</p></font></td> | ||
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+ | <td colspan="3" style="background-color:#FFF2B5"><font face="Trebuchet MS" size="6" color="#A66B38">Recombinant Phagemid Construction</font></td> | ||
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+ | <td valign="top"><font size="3" face="Verdana" color="#333"><p>After constructed our CRISPR system, we ligated it with a red fluorescent reporter gene (<a href="http://parts.igem.org/Part:BBa_I13521" target="_blank">BBa_I13521</a>), so once our system is spread, all bacterium get this system will become red. Finally, we put whole system into phagemid pBluescript II SK(-), and get the recombinant phagemid for M13 infection.</p></font></td> | ||
+ | <td width="15"> </td> | ||
+ | <td width="60%"><table width="90%" border="1" cellspacing="0" cellpadding="0" align="center"> | ||
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+ | <td><img src="https://static.igem.org/mediawiki/2014/4/4b/TCU_SD_10572757_390371167783707_1137689428_o.jpg" width="100%" /></td> | ||
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+ | <td align="center"><font size="3" face="Verdana"><strong>Fig.2</strong></font></td> | ||
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Revision as of 18:56, 15 October 2014
System Design |
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