Team:TCU Taiwan/Project

From 2014.igem.org

(Difference between revisions)
 
(29 intermediate revisions not shown)
Line 137: Line 137:
     <ul>
     <ul>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Team"><font size="3">Members</font></a></li>
 +
      <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Achievements"><font size="3">Achievements</font></a></li>
       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
       <li><a href="https://igem.org/Team.cgi?id=1473" target="_blank"><font size="3">Official Team Profile</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li>
       <li><a href="https://2014.igem.org/Team:TCU_Taiwan/Contact"><font size="3">Contact</font></a></li>
Line 239: Line 240:
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
<a href="#topic1"
<a href="#topic1"
-
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 1</font></a> </td>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Protagonist</font></a> </td>
</tr>
</tr>
Line 246: Line 247:
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
<a href="#topic2"
<a href="#topic2"
-
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 2</font></a> </td>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Super Cast</font></a> </td>
</tr>
</tr>
Line 252: Line 253:
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
-
<a href="#topic3"
+
<a href="#Story"
-
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Topic 3</font></a> </td>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">The Story</font></a> </td>
</tr>
</tr>
Line 259: Line 260:
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
<td style="border:0px solid black;" align="center"  width="33%" height ="35px"  
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
onMouseOver="this.bgColor='#99CC69'" onMouseOut="this.bgColor='#FFCE5B'" bgColor=#FFCE5B>   
-
<a href="#references"
+
<a href="#Program"
-
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">References</font></a> </td>
+
style="color:#0B6D78; text-decoration: none;"><font face="Trebuchet MS" size="5">Program</font></a> </td>
</tr>
</tr>
 +
<tr>
<tr>
   <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
   <td align="right" height ="35px"><img src="https://static.igem.org/mediawiki/2014/b/b8/TCU-Phage.png" width="30%"/></td>
Line 278: Line 280:
<tr ><td coslpan="3" height="35px"></td></tr>
<tr ><td coslpan="3" height="35px"></td></tr>
-
<tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
+
<tr><td colspan="3" style="background-color:#FFF2B5" height="20px"> <font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Abstract</font> </td> </tr>
<tr>
<tr>
   <td valign="top" height="10px">&nbsp;</td>
   <td valign="top" height="10px">&nbsp;</td>
Line 307: Line 309:
<table width="975px" align="center">
<table width="975px" align="center">
-
<tr> <td> <h3 id="topic1">Topic 1</h3> </td> </tr>
+
<tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic1"><font face="Trebuchet MS" size="6" color="#A66B38">Protagonist</font></h3> </td> </tr>
<tr>
<tr>
<!-- first column -->
<!-- first column -->
-
<td width="30%">
+
<td width="50%" colspan="3">
-
<p>&nbsp;</p>
+
<font size="4" face="Verdana" color="#333"><p>Trogene horse is a lovely wooden horse, but he carries a super  weapon inside, and this weapon can help greek soldiers defeat Troy city.</p></font>
</td>
</td>
-
<td width="5%"></td>
+
 
<!-- second column -->
<!-- second column -->
-
<td width="30%">
 
-
<p>&nbsp;</p>
 
-
</td>
 
-
<td width="5%"></td>
+
 
 +
<td width="30"></td>
<!-- third column -->
<!-- third column -->
-
<td width="30%">
+
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
+
<img src="https://static.igem.org/mediawiki/2014/c/c2/TCU-Logo-up.png" width="490" height="381">
</td>
</td>
Line 338: Line 338:
<table width="975px" align="center">
<table width="975px" align="center">
-
<tr> <td> <h3 id="topic2">Topic 2</h3>  </td> </tr>
+
<tr> <td style="background-color:#FFF2B5" height="20px"> <h3 id="topic2"><font face="Trebuchet MS" size="6" color="#A66B38" id="abstract">Super Cast</font></h3>  </td> </tr>
<tr>
<tr>
<!-- first column -->
<!-- first column -->
-
<td width="30%">
+
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
+
<img src="https://static.igem.org/mediawiki/2014/c/cc/TCU-Anti.png" width="200px">
</td>
</td>
-
<td width="5%"></td>
+
<td width="10">&nbsp;</td>
 +
<td><font size="5" face="Verdana" color="#0092C7"><p>Antibiotics:</p></font><font size="4" face="Verdana" color="#333">
 +
  <p>A  group of greek soldiers who is eager to conquest troy city. </p>
 +
</font></td>
 +
<td width="30">&nbsp;</td>
<!-- second column -->
<!-- second column -->
-
<td width="30%">
+
<td align="center">
-
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="300px">
+
<img src="https://static.igem.org/mediawiki/2014/d/d3/TCU_Igem_cell.png" width="120">
</td>
</td>
-
<td width="5%"></td>
+
<td width="10">&nbsp;</td>
<!-- third column -->
<!-- third column -->
<td width="30%">
<td width="30%">
-
<p>&nbsp;</p>
+
<font size="5" face="Verdana" color="#0092C7"><p>Bacterium:</p></font><font size="4" face="Verdana" color="#333">
 +
<p>Troy  citizens who lives happily under the protection of troy wall. </p>
 +
</font>
</td>
</td>
</tr>
</tr>
Line 372: Line 378:
<table width="975px" align="center">
<table width="975px" align="center">
-
<tr> <td> <h3 id="topic3">Topic 3</h3>  </td> </tr>
+
<tr> <td> <h3 id="topic3">&nbsp;</h3>  </td> </tr>
<tr>
<tr>
<!-- first column -->
<!-- first column -->
-
<td width="65%">
+
<td align="center" width="30%">
-
<p>&nbsp;</p>
+
<p><img src="https://static.igem.org/mediawiki/2014/7/7e/TCU_WALL.png" width="300" height="300" /></p>
</td>
</td>
-
<td width="5%"></td>
+
<td width="30"></td>
<!-- second column -->
<!-- second column -->
-
<td width="30%">
+
<td><font size="5" face="Verdana" color="#0092C7"><p>Troy Wall: </p></font><font size="4" face="Verdana" color="#333">
-
<img src="https://static.igem.org/mediawiki/2014/b/b7/2014placeholderimage.jpg" width="300px" height="200px">
+
  <p>A great wall of Troy city, it is the nightmare of  greek soldiers. </p>
-
</td>
+
</font></td>
 +
</tr>
 +
<tr>
 +
  <td align="center" height="30">&nbsp;</td>
 +
  <td></td>
 +
  <td>&nbsp;</td>
 +
</tr>
 +
<tr>
 +
  <td align="center"><img src="https://static.igem.org/mediawiki/2014/1/11/TCU_GODDESS.png" width="300" height="300" /></td>
 +
  <td></td>
 +
  <td><font size="5" face="Verdana" color="#0092C7"><p>TCU-Taiwan:</p></font><font size="4" face="Verdana" color="#333">
 +
    <p>Greek  soldiers&rsquo; goddess who designed secret agents can break troy wall and hide them  inside the Trogene Horse. With this, Trogene Horse become a powerful weapon of  greek soldiers.</p>
 +
  </font></td>
</tr>
</tr>
Line 396: Line 414:
</table>
</table>
<!-- end of 2 column content -->
<!-- end of 2 column content -->
 +
<table width="975" border="0" cellspacing="0" cellpadding="0" align="center">
 +
  <tr>
 +
    <td height="30">&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td style="background-color:#FFF2B5" height="20px" align="center"><h3 id="Story"><font face="Trebuchet MS" size="6" color="#A66B38">The Story</font></h3> </td>
 +
  </tr>
 +
  <tr>
 +
    <td height="15">&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td align="center">
 +
    <video width="640" height="360" controls>
 +
<source src="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov" type="video/mp4">
 +
<iframe width="853" height="480" src="//www.youtube.com/embed/jMb2o3Bwiq8?showinfo=0" frameborder="0" allowfullscreen></iframe>
 +
<object>
 +
Your browser does not support the HTML5 video tag!
 +
<a href="https://static.igem.org/mediawiki/2014/9/95/TCU-Taiwan_An_Introduction_of_Trogene_Horse_%28720p%29.mov"><br>Download the video.</a>
 +
</object>
 +
</video>
 +
 
 +
    </td>
 +
  </tr>
 +
</table>
<!-- references -->
<!-- references -->
Line 402: Line 444:
<tr ><td height="35px"></td></tr>
<tr ><td height="35px"></td></tr>
-
<tr><td> <h3 id="references">References</h3>
+
 
-
<ul>
+
-
<li></li>
+
-
</ul>
+
-
</td>
+
-
</tr>
+
</tr></table>
</tr></table>
<table width="975" border="0" cellspacing="0" cellpadding="0" align="center">
<table width="975" border="0" cellspacing="0" cellpadding="0" align="center">
   <tr>
   <tr>
-
     <td  style="background-color:#FFF2B5" height="20px"><font face="Trebuchet MS" size="7" color="#A66B38" id="abstract">Program</font></td>
+
     <td  style="background-color:#FFF2B5" height="20px" align="center"><font face="Trebuchet MS" size="6" color="#A66B38" id="Program">Program</font></td>
   </tr>
   </tr>
   <tr>
   <tr>
Line 422: Line 459:
       <tr>
       <tr>
         <td align="center" width="15">&nbsp;</td>
         <td align="center" width="15">&nbsp;</td>
-
         <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128" height="128" onmouseover="Partsbox.color='#90B849'" onmouseout="Partsbox.color='#333'"/></td>
+
         <td align="center" width="250" height="200"><a href="https://2014.igem.org/Team:TCU_Taiwan/Parts"><img src="https://static.igem.org/mediawiki/2014/e/e5/TCU-Part-logo.png" alt="Parts" width="128" onmouseover="this.width=this.width*1.2;Partsbox.color='#90B849';" onmouseout="this.width=this.width/1.2;Partsbox.color='#333';"/></a></td>
         <td width="20">&nbsp;</td>
         <td width="20">&nbsp;</td>
-
         <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128" height="128"  alt="System Design"/></td>
+
         <td align="center" width="250"><a href="https://2014.igem.org/Team:TCU_Taiwan/SystemDesign"><img src="https://static.igem.org/mediawiki/2014/4/4d/TCU_Blueprint-128.png" width="128"alt="System Design" onmouseover="this.width=this.width*1.2;Systembox.color='#90B849';" onmouseout="this.width=this.width/1.2;Systembox.color='#333';"/></a></td>
         <td width="20">&nbsp;</td>
         <td width="20">&nbsp;</td>
-
         <td align="center" width="250"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" height="128" /></td>
+
         <td align="center" width="250"><a href="https://2014.igem.org/Team:TCU_Taiwan/M13Phage"><img src="https://static.igem.org/mediawiki/2014/2/24/TCU_Microscope-128.png" alt="M13 Phage" width="128" onmouseover="this.width=this.width*1.2;M13box.color='#90B849';" onmouseout="this.width=this.width/1.2;M13box.color='#333';" /></a></td>
         <td align="center" width="15">&nbsp;</td>
         <td align="center" width="15">&nbsp;</td>
       </tr>
       </tr>
Line 449: Line 486:
       <tr>
       <tr>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
-
         <td height="50">&nbsp;</td>
+
         <td height="35">&nbsp;</td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
Line 458: Line 495:
       <tr>
       <tr>
         <td align="center">&nbsp;</td>
         <td align="center">&nbsp;</td>
-
         <td align="center"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" height="128" /></td>
+
         <td align="center" width="250" height="200"><a href="https://2014.igem.org/Team:TCU_Taiwan/CRISPR"><img src="https://static.igem.org/mediawiki/2014/4/43/TCU_Gear-128.png" alt="CRISPR" width="128" onmouseover="this.width=this.width*1.2;CRISPRbox.color='#90B849';" onmouseout="this.width=this.width/1.2;CRISPRbox.color='#333';"/></a></td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
-
         <td align="center"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" height="128" /></td>
+
         <td align="center"><a href="https://2014.igem.org/Team:TCU_Taiwan/FutureWorks"><img src="https://static.igem.org/mediawiki/2014/4/4a/TCU_1413400508_Briefcase.png" alt="Future Works" width="128" onmouseover="this.width=this.width*1.2;Futurebox.color='#90B849';" onmouseout="this.width=this.width/1.2;Futurebox.color='#333';"/></a></td>
         <td>&nbsp;</td>
         <td>&nbsp;</td>
-
         <td align="center"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" alt="Human Pratics" width="128" height="128" /></td>
+
         <td align="center"><a href="https://2014.igem.org/Team:TCU_Taiwan/HumanPratics"><img src="https://static.igem.org/mediawiki/2014/d/dd/TCU-Human-logo.png" alt="Human Pratics" width="128" onmouseover="this.width=this.width*1.2;Humanbox.color='#90B849';" onmouseout="this.width=this.width/1.2;Humanbox.color='#333';"/></a></td>
         <td align="center">&nbsp;</td>
         <td align="center">&nbsp;</td>
       </tr>
       </tr>

Latest revision as of 13:32, 17 October 2014


 
Project
 
   
Trogene Horse  
Abstract
Protagonist
Super Cast
The Story
Program
Abstract
   

To access this, we use a special phagemid for phage display. The phagemid contains a additional phage replication origin. When helper phage (a phage whose own ori is knock-out) infection take place, phagemid can be packaged as genome for progeny. Since we put an antibiotic resistance gene targeting CRISPR system on our phagemid, this phage display can spread our CRISPR among bacterium nearby and remove their resistance efficiently.

Trogene Horse is a new concept combining phage therapy with CRISPR, and this combination can solve both safety concern(phage therapy) and efficiency problem(CRISPR). We believe our project can deal with bacterial resistance problem on agriculture, and hope it can access on medicine one day.

Protagonist

Trogene horse is a lovely wooden horse, but he carries a super weapon inside, and this weapon can help greek soldiers defeat Troy city.

Super Cast

 

Antibiotics:

A group of greek soldiers who is eager to conquest troy city.

   

Bacterium:

Troy citizens who lives happily under the protection of troy wall.

 

Troy Wall:

A great wall of Troy city, it is the nightmare of greek soldiers.

   

TCU-Taiwan:

Greek soldiers’ goddess who designed secret agents can break troy wall and hide them inside the Trogene Horse. With this, Trogene Horse become a powerful weapon of greek soldiers.

 

The Story

 
Program
 
  Parts   System Design   M13 Phage  
             
  Parts   System Design   M13 Phage  
             
  CRISPR   Future Works   Human Pratics  
             
  CRISPR   Future Work   Human Pratics  
 
^


    
Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

Lost the way? Use it to help you if you're lost.

^