Team:TCU Taiwan/Notebook

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<body style="background:#ffffff">

  <img src="TCU-Notebook-logo.png" width="128" height="128">
Notebook
<img src="TCU-Logo-up.png" width="90%">  
<tbody>
<tbody> </tbody>

<tr><td colspan="3"background="#F3E4C2"><img src="TCU-Line.png" width="100%"></td></tr>

</tbody></table>



iGEM materials
<fieldset>
 <legend class="qnum" align="center">Bacteria medium
</legend>

*LB(Luria-Bertani) broth: 1% Trytone, 1% NaCl, 0.5% Yeast extract in the ddH2O

  • LB agar plate:LB broth add 1.5% agar.If you want to add selection marker example for ampicillin or chloramphenicol. To culture E.coli, ampicillin is added 100 mg/ml for usage, chloramphenicol is added 30 mg/ml for usage, kanamycin is added 70 mg/ml for usage

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Plasmid isolation
</legend>

*Solution I: 50 mM glucose、25 mM Tris-HCl (pH 8.0)、10 mM EDTA

  • Solution II:0.2N NaOH and 1% SDS(Sodium dodecyl sulfate ) mix
  • Solution III: 5 M potassium acetate (pH4.8), 11.5% glacial acetic acid
  • RNase A(10 mg/ml)
  • Phenol chloroform
  • Chloroform
  • 95% Ethanol
  • Tris-EDTA(TE)buffer:10 mM Tris-Cl(pH 7.5) 1 mM EDTA
  • pBluescript SK(-)
  • BBa_K1218011
  • BBa_K914003
  • BBa_I13521

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Quick screening
</legend>

*Quick screening buffer(Tris-Cl pH 12.6, 3% SDS)

  • Phenol chloroform
  • Chloroform

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Enzyme digestion

</legend>

*T4 ligase

  • 10X ligation buffer
  • EcoRI
  • PstI
  • SpeI
  • XbaI
  • BSA 0.1%

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Bacteria strains and others
</legend>

*DH5a
*JM101
*0.8% and 2% agarose gel
*95% Ethanol
*6X loading dye
*1.5 ml centrifuge tube
*250 ml flask
*M13KO7(1*1011 pfu/ml)from NEW ENGLAND BioLabs

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Polymerase chain reaction
</legend>

*Top DNA polymerase

  • dNTP(dATP, dCTP, dGTP, dTTP) 250 mM
  • Tris-HCl(pH9.0) 10 mM
  • KCl 30 mM
  • MgCl2 1.5 mM

   </fieldset>
<fieldset>
 <legend class="qnum" align="center">Instruments
</legend>

*VCM-620, laminal flow

  • Biofuge pico, Heraeus
  • OS1500-H, TSK Oribital Shaking Iucubator
  • Biowave
  • Vortex-GENE 2
  • Autoclave AS-1060L
  • GenAmp PCR System 2400, Applied Biosystems
  • BU-420, YIHDER
  • 5804R, eppendorf Centrifuge

   </fieldset>
Life Records
   
 
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Study Together
 
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Conference in NCTU
 
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Trip in Chihsingtan Beach
 
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