Week 1 6/22~6/28
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 2 6/29~7/5
experimental part:
Successfully transform biobrick BBa_I13521、BBa_ K914003、BBa_ K1218011 into DH5α (plasmid: pSB1C3
Extract pSB1C3 after incubation
Use Enzyme digestion(PstI and EcoRI) to confirm whether pSB1C3 is successfully extracted
Prepared Cm. plate、LB. plate
Prepared competent cell
other part:
Get foundation from nearby store (yeah!!!!!)
Decided to have iGEM contest in local school as human practice
Others don’t think E.Maat is a good name, under re-consideration
Week 3 7/6~7/12
experimental part:
Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011
Prepare Cm. plate、LB. plate
other part:
Something wrong with gRNA for AmpR, redesign
Second project theme came out by Denise and Regina: Trogene horse. Needs later discuss to decided which one as theme( E.Maat or Trogene Horse)
Try to get foundation from Biotechnology company on e-mail.
Prepare for igem conference in NCTU-Formosa (ppt)
Week 4 7/13~7/19
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 5 7/20~7/26
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 6 7/27~8/2
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 7 8/3~8/7
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 8 8/10~16
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 9 8/17~8/23
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 10 8/24~8/30
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 11 8/31~9/6
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 12 9/7~9/13
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 13 9/14~9/20
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 14 9/21~9/27
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
Week 15 9/28~10/4
experimental part:
Prepare for the competent cell
BoBo and Ted decided to multiply plasmid within bacteria but not PCR, like this:
Transformation ( Cas9 in pSB1C3 into DH5α)
Incubate DH5α with pSB1C3
Extract plasmid with pSB1C3 from DH5α
other part:
Prepare Human practice (ppt)
Practice presentation
Design gRNA for AmpR
Discuss about project. Project Name is E.Maat for now (came up by Sharon)
Order restraction enzyme(PstI、EcoRI、XbaI、SpeI)
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