Team:Sumbawagen/Parts

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<h1 >WELCOME TO iGEM 2014! </h1>
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                class='selected'       ><a href="/Team:Dundee/Team">Page              </a></li>
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<p>Your team has been approved and you are ready to start the iGEM season!
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              <li
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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                class='new'       ><a href="/wiki/index.php?title=Talk:Team:Dundee/Team&amp;action=edit&amp;redlink=1">Discussion              </a></li>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Parts&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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                    ><a href="/wiki/index.php?title=Team:Dundee/Team&amp;action=edit">View source              </a></li>
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<h1 class="firstHeading">Team:Sumbawagen/Team</h1>
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<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3>
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    <title>iGEM Sumbawagen 2014 &middot; Econey</title>
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<a href="https://2014.igem.org/Team:Sumbawagen/Parts"style="color:#000000"> Parts</a></td>
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            <a class="brand" href="https://2014.igem.org/Team:Sumbawagen/Parts">Sumbawagen</a>
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                <li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li>
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<a href="https://2014.igem.org/Team:Sumbawagen/Safety"style=" color:#000000"> Safety </a></td>
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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                      <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey"> E-coney Project </a></li>
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                    </ul>
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                </li>
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<td style="border:1px solid black;" align="center"  height ="45px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
 
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<a href="https://2014.igem.org/Team:Sumbawagen/Attributions"style="color:#000000"> Attributions </a></td>
 
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                  <a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>                           
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Daily Notes</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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                    <!--<li><a href="#">Improvements</a></li>-->
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
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                    <li><a href="http:https://igem.org/Team.cgi">Team Information</a></li>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
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                    <li class="nav-header">Social Media</li>
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                    <li><a href="http://sumbawagen.blogspot.com/">Blog</a></li>
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                <li class="dropdown">
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                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a>
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                    <li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy">
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<strong>Policy & Practices</strong>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
 +
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responds and supports</a></li>
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An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will submit them to the <a href="http://partsregistry.org"> Registry of Standard Biological Parts</a>. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. 
 
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<strong>Note that if you want to document a part you need to document it on the <a href="http://partsregistry.org Registry"> Registry</a>, not on your team wiki.</strong> Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
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As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
 
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The information needed to initially create a part on the Registry is:
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<ol>
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<li>Part Name</li>
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<h2>Composite</h2>
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<strong>BBa_K1508003 (IIA(Glc) + double terminator)</strong><br>
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<li>Creator</li>
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This biobrick contains gene coding for  IIA(Glc) (BBa_K1508002), followed by double terminator (BBa_B0015). IIA(Glc) is glucose-specific phosphocarrier protein of the phosphoenolpyruvate : glucose phosphotransferase system (PTS). This circuit inserted to the upstream part of plasmid Bba_J04450. We hypothesized that by over-expressing the enzyme, more glucose can be uptook compared to normal condition.<br>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part <a href="http://parts.igem.org/Part:BBa_K404003">BBa_K404003</a> for an excellent example of a highly characterized part.
 
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<h2>Coding </h2>
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You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry"> Add a Part to the Registry</a> link.
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<strong>BBa_K1508002 (IIA(Glc) - <i>E.coli</i> glucose-specific phosphotransferase enzyme IIA)</strong><br>
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Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in <i>E. coli</i>, we will obtain different range of glucose standard curve in our measurement system.<br>
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<h2>BBa_K1508000 (IIA(Glc)</h2>
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<strong>BBa_K1508000 (IIA(Glc) - Glucose-specific phosphotransferase enzyme IIA component from <i>E. coli</i>)</strong>
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<p>IIA(Glc) - previously known as III(Glc) - is glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (PTS). Therefore, we hypothesized that by over-expressing the enzyme, more glucose can be uptook compared to normal condition. </p>
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Any parts your team has created will appear in this table below:</td></tr>
 
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<groupparts>iGEM013 Sumbawagen</groupparts>
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Latest revision as of 01:35, 18 October 2014

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Sumbawagen

Biobrick


Composite

BBa_K1508003 (IIA(Glc) + double terminator)
This biobrick contains gene coding for IIA(Glc) (BBa_K1508002), followed by double terminator (BBa_B0015). IIA(Glc) is glucose-specific phosphocarrier protein of the phosphoenolpyruvate : glucose phosphotransferase system (PTS). This circuit inserted to the upstream part of plasmid Bba_J04450. We hypothesized that by over-expressing the enzyme, more glucose can be uptook compared to normal condition.

Coding

BBa_K1508002 (IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA)
Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in E. coli, we will obtain different range of glucose standard curve in our measurement system.

BBa_K1508000 (IIA(Glc)

BBa_K1508000 (IIA(Glc) - Glucose-specific phosphotransferase enzyme IIA component from E. coli)

IIA(Glc) - previously known as III(Glc) - is glucose-specific phosphocarrier protein of the phosphoenolpyruvate:glucose phosphotransferase system (PTS). Therefore, we hypothesized that by over-expressing the enzyme, more glucose can be uptook compared to normal condition.

Sponsors




Retrieved from "https://2013.igem.org/Team:Dundee/Project/MopQuantifying"

Retrieved from "http://2014.igem.org/Team:Sumbawagen/Parts"