Team:Sumbawagen/Notebook/protocol7

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                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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Latest revision as of 09:22, 19 February 2015

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Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 7. Ligation

1. Ligation reaction was as follow:

  • Insert DNA, 2,5 ul*
  • Plasmid DNA, 1.5 ul*
  • Ligation kit (Takara), 5 ul
  • Total volume, 10 ul
    * final quantity insert DNA > plasmid DNA

2. Samples were incubated at 4 oC for overnight (inside a refrigerator).