Team:Sumbawagen/Notebook/protocol7

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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
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                    <li class="nav-header">Daily Notes</li>
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                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
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                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
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                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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                    <li><a href="http:http://igem.org/Team.cgi">Team Information</a></li>
 
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responds and supports</a></li>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
                                                            
                                                            

Revision as of 03:08, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 7. Ligation

1. Ligation reaction was as follow:

  • Insert DNA, 2,5 ul*
  • Plasmid DNA, 1.5 ul*
  • Ligation kit (Takara), 5 ul
  • Total volume, 10 ul
    * final quantity insert DNA > plasmid DNA

2. Samples were incubated at 4 oC for overnight (inside a refrigerator).