Team:Sumbawagen/Notebook/protocol7

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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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<li>Plasmid DNA, 1.5 ul*</li>
<li>Plasmid DNA, 1.5 ul*</li>
<li>Ligation kit (Takara), 5 ul</li>
<li>Ligation kit (Takara), 5 ul</li>
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Total volume,                 10 ul</li>
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Total volume,                 10 ul</li><br>
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* final quantity insert DNA > plasmid DNA  
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* final quantity insert DNA > plasmid DNA </li>
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</p></ul>
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<p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p>
<p>2. Samples were incubated at 4 oC for overnight (inside a refrigerator).</p>

Latest revision as of 09:22, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 7. Ligation

1. Ligation reaction was as follow:

  • Insert DNA, 2,5 ul*
  • Plasmid DNA, 1.5 ul*
  • Ligation kit (Takara), 5 ul
  • Total volume, 10 ul
    * final quantity insert DNA > plasmid DNA

2. Samples were incubated at 4 oC for overnight (inside a refrigerator).