Team:Sumbawagen/Notebook/protocol5

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Latest revision as of 09:19, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 5. Restriction enzymes digestion

1. Restriction enzymes digestion was done using Fast Digest product (Thermoscientific) using the following composition:

  • DNA sample (1-3 ug), 15 ul
  • Fast Digest restriction enzyme #1, 2 ul
  • Fast Digest restriction enzyme #2, 2 ul
  • 10x buffer, 3 ul
  • Sterilized distilled water, 6 ul
  • Total volume, 30 ul

2. Samples were incubated at 37 °C for more than 1 hour (typically 3 hours).

3. When necessary, samples were electrophoresed using protocol “3. Electrophoresis”(typically 3 hours).

4. Samples were purified using protocol “4. DNA purification”.