http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&feed=atom&action=history
Team:Sumbawagen/Notebook/protocol13 - Revision history
2024-03-28T10:42:43Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&diff=406195&oldid=prev
Indah at 09:27, 19 February 2015
2015-02-19T09:27:26Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 09:27, 19 February 2015</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 212:</td>
<td colspan="2" class="diff-lineno">Line 212:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook3">Mobile Programming</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li class="divider"></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <!--<li><a href="#">Improvements</a></li>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <!--<li><a href="#">Improvements</a></li>--></div></td></tr>
</table>
Indah
http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&diff=406125&oldid=prev
Isro at 03:05, 19 February 2015
2015-02-19T03:05:36Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:05, 19 February 2015</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 171:</td>
<td colspan="2" class="diff-lineno">Line 171:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </button></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </button></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a class="brand" href="https://2014.igem.org/Team:Sumbawagen<del class="diffchange diffchange-inline">/Parts</del>">Sumbawagen</a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a class="brand" href="https://2014.igem.org/Team:Sumbawagen">Sumbawagen</a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="nav-collapse collapse"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <div class="nav-collapse collapse"></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 181:</td>
<td colspan="2" class="diff-lineno">Line 181:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/<del class="diffchange diffchange-inline">Econey</del>"> <del class="diffchange diffchange-inline">E-coney </del>Project </a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/<ins class="diffchange diffchange-inline">Econey_Project</ins>"> <ins class="diffchange diffchange-inline">Econey </ins>Project </a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 196:</td>
<td colspan="2" class="diff-lineno">Line 196:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li><a href="https://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 207:</td>
<td colspan="2" class="diff-lineno">Line 208:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook"><del class="diffchange diffchange-inline">Daily Notes</del></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li class="nav-header">Daily Notes</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook"><ins class="diffchange diffchange-inline">Policy & Practices</ins></a<ins class="diffchange diffchange-inline">></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook3">Mobile Programming</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li class="divider"</ins>></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <!--<li><a href="#">Improvements</a></li>--></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <!--<li><a href="#">Improvements</a></li>--></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 219:</td>
<td colspan="2" class="diff-lineno">Line 225:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul class="dropdown-menu"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="http:https://igem.org/Team.cgi">Team Information</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 259:</td>
<td colspan="2" class="diff-lineno">Line 264:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support"><del class="diffchange diffchange-inline">Responds </del>and supports</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support"><ins class="diffchange diffchange-inline">Responses </ins>and supports</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
</table>
Isro
http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&diff=386627&oldid=prev
Adel at 02:07, 18 October 2014
2014-10-18T02:07:36Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:07, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 301:</td>
<td colspan="2" class="diff-lineno">Line 301:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>5. The rest of the sample in the tube was centrifuged at 6,000 rpm, 10 min, room temperature.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>5. The rest of the sample in the tube was centrifuged at 6,000 rpm, 10 min, room temperature.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>6. 50 ul of supernatant was aliquoted and used as “soluble protein’ sample.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>6. 50 ul of supernatant was aliquoted and used as “soluble protein’ sample.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>7. Protein <del class="diffchange diffchange-inline">concentrations </del>of “total protein” and “soluble protein” were estimated using Lowry method based on the kit from Biorad. We have no UV spectrophotometer for measurement of real concentration at 750 nm. But according to our chief instructor Dr. Arief previous experiment, the concentration of “soluble protein” will be about 1,000 – 2,000 ug/ml, while the concentration of “total protein” will be about 10,000 – 20,000 ug/ml.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>7. Protein <ins class="diffchange diffchange-inline">concentration </ins>of “total protein” and “soluble protein” were estimated using Lowry method based on the kit from Biorad. We have no UV spectrophotometer for measurement of real concentration at 750 nm. But according to our chief instructor Dr. Arief previous experiment, the concentration of “soluble protein” will be about 1,000 – 2,000 ug/ml, while the concentration of “total protein” will be about 10,000 – 20,000 ug/ml.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>8. “Soluble protein” sample was used directly, while “total protein” sample was diluted 1/10th to obtain samples with roughly the same concentration for SDS-PAGE.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>8. “Soluble protein” sample was used directly, while “total protein” sample was diluted 1/10th to obtain samples with roughly the same concentration for SDS-PAGE.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>9. 15 ul sample was added with 15 ul dye containing SDS and Coomassie Brilliant Blue (CBB) in 500 ul polypropylene tube, then the tube was put in boiled water for 15 min</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>9. 15 ul sample was added with 15 ul dye containing SDS and Coomassie Brilliant Blue (CBB) in 500 ul polypropylene tube, then the tube was put in boiled water for 15 min</p></div></td></tr>
</table>
Adel
http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&diff=386372&oldid=prev
Adel at 02:05, 18 October 2014
2014-10-18T02:05:09Z
<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:05, 18 October 2014</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 301:</td>
<td colspan="2" class="diff-lineno">Line 301:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>5. The rest of the sample in the tube was centrifuged at 6,000 rpm, 10 min, room temperature.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>5. The rest of the sample in the tube was centrifuged at 6,000 rpm, 10 min, room temperature.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>6. 50 ul of supernatant was aliquoted and used as “soluble protein’ sample.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>6. 50 ul of supernatant was aliquoted and used as “soluble protein’ sample.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>7. Protein <del class="diffchange diffchange-inline">concentration </del>of “total protein” and “soluble protein” were estimated using Lowry method based on the kit from Biorad. We have no UV spectrophotometer for measurement of real concentration at 750 nm. But according to our chief instructor Dr. Arief previous experiment, the concentration of “soluble protein” will be about 1,000 – 2,000 ug/ml, while the concentration of “total protein” will be about 10,000 – 20,000 ug/ml.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>7. Protein <ins class="diffchange diffchange-inline">concentrations </ins>of “total protein” and “soluble protein” were estimated using Lowry method based on the kit from Biorad. We have no UV spectrophotometer for measurement of real concentration at 750 nm. But according to our chief instructor Dr. Arief previous experiment, the concentration of “soluble protein” will be about 1,000 – 2,000 ug/ml, while the concentration of “total protein” will be about 10,000 – 20,000 ug/ml.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>8. “Soluble protein” sample was used directly, while “total protein” sample was diluted 1/10th to obtain samples with roughly the same concentration for SDS-PAGE.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>8. “Soluble protein” sample was used directly, while “total protein” sample was diluted 1/10th to obtain samples with roughly the same concentration for SDS-PAGE.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>9. 15 ul sample was added with 15 ul dye containing SDS and Coomassie Brilliant Blue (CBB) in 500 ul polypropylene tube, then the tube was put in boiled water for 15 min</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>9. 15 ul sample was added with 15 ul dye containing SDS and Coomassie Brilliant Blue (CBB) in 500 ul polypropylene tube, then the tube was put in boiled water for 15 min</p></div></td></tr>
</table>
Adel
http://2014.igem.org/wiki/index.php?title=Team:Sumbawagen/Notebook/protocol13&diff=385811&oldid=prev
Adel: Created page with "<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <meta name="genera..."
2014-10-18T01:59:46Z
<p>Created page with "<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <meta name="genera..."</p>
<p><b>New page</b></p><div><html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" /><br />
<meta name="generator" content="MediaWiki 1.16.5" /><br />
<link rel="shortcut icon" href="/favicon.ico" /><br />
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" /><br />
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:Dundee/Team&amp;action=creativecommons" rel="meta" /><br />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" /><br />
<link rel="alternate" type="application/atom+xml" title="2013.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:Dundee/Team - 2013.igem.org</title><br />
<style type="text/css" media="screen, projection">/*<![CDATA[*/<br />
@import "/wiki/skins/common/shared.css?270";<br />
@import "/wiki/skins/igem/main.cs70";<br />
/*]]>*/</style><br />
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" /><br />
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]--><br />
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]--><br />
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]--><br />
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]--><br />
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script><br />
<meta http-equiv="imagetoolbar" content="no" /><![endif]--><br />
<br />
<script><br />
var skin="igem",<br />
stylepath="/wiki/skins",<br />
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",<br />
wgArticlePath="/$1",<br />
wgScriptPath="/wiki",<br />
wgScriptExtension=".php",<br />
wgScript="/wiki/index.php",<br />
wgVariantArticlePath=false,<br />
wgActionPaths={},<br />
wgServer="https://2014.igem.org",<br />
wgCanonicalNamespace="",<br />
wgCanonicalSpecialPageName=false,<br />
wgNamespaceNumber=0,<br />
wgPageName="Team:Dundee/Team",<br />
wgTitle="Team:Dundee/Team",<br />
wgAction="view",<br />
wgArticleId=240,<br />
wgIsArticle=true,<br />
wgUserName=null,<br />
wgUserGroups=null,<br />
wgUserLanguage="en",<br />
wgContentLanguage="en",<br />
wgBreakFrames=false,<br />
wgCurRevisionId=325506,<br />
wgVersion="1.16.5",<br />
wgEnableAPI=true,<br />
wgEnableWriteAPI=true,<br />
wgSeparatorTransformTable=["", ""],<br />
wgDigitTransformTable=["", ""],<br />
wgMainPageTitle="Main Page",<br />
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},<br />
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},<br />
wgSiteName="2013.igem.org",<br />
wgCategories=[],<br />
wgMWSuggestTemplate="https://2014.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",<br />
wgDBname="2013_igem_org",<br />
wgSearchNamespaces=[0],<br />
wgMWSuggestMessages=["with suggestions", "no suggestions"],<br />
wgRestrictionEdit=[],<br />
wgRestrictionMove=[];<br />
</script> <br />
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script><br />
<!-- Head Scripts --><br />
<script src="/wiki/skins/common/ajax.js?270"></script><br />
<script src="/wiki/skins/common/mwsuggest.js?270"></script><br />
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;gen=js&amp;useskin=igem"><!-- site js --></script><br />
<!-- jQuery Javascript --><br />
<script type='text/javascript' src ='/common/jquery-latest.min.js'></script><br />
<script type='text/javascript' src ='/common/tablesorter/jquery.tablesorter.min.js'></script><br />
<link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' /><br />
<link rel='stylesheet' type='text/css' href='/common/table_styles.css' /><br />
<link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' /><br />
<script type='text/javascript' src ='/forum/forum_scripts.js'></script><br />
</head><br />
<br />
<!-- end p-logo --><br />
<script type="text/javascript"> if (window.isMSIE55) fixalpha(); </script><br />
<br />
<br />
<br />
<div id="menubar" class='left-menu noprint'><br />
<ul><br />
<li <br />
class='selected' ><a href="/Team:Dundee/Team">Page </a></li><br />
<li <br />
class='new' ><a href="/wiki/index.php?title=Talk:Team:Dundee/Team&amp;action=edit&amp;redlink=1">Discussion </a></li><br />
<li <br />
><a href="/wiki/index.php?title=Team:Dundee/Team&amp;action=edit">View source </a></li><br />
<li <br />
><a href="/wiki/index.php?title=Team:Dundee/Team&amp;action=history">History </a></li><br />
<li style='color:white;cursor:default'>teams</li><br />
</ul><br />
</div> <!-- end menubar (left) --><br />
<br />
<div class="right-menu noprint" id="menubar"><br />
<ul><br />
<li id="pt-login"><a href="/wiki/index.php?title=Special:UserLogin&amp;returnto=Team:Dundee/Team" title="You are encouraged to log in; however, it is not mandatory [o]" accesskey="o">Log in</a></li> </ul><br />
</div><!-- end right menubar --><br />
<br />
<div id="search-controls" class="noprint"><br />
<form action="/Special:Search" id="searchform"><br />
<input id="searchInput" name="search" type="text" title="Search 2014.igem.org [f]" accesskey="f" value="" /><br />
<input type='submit' name="go" class="searchButton" id="searchGoButton" value="Go" title="Go to a page with this exact name if exists" />&nbsp;<br />
<input type='submit' name="fulltext" class="searchButton" id="mw-searchButton" value="Search" title="Search the pages for this text" /><br />
</form><br />
</div> <!-- close search controls --><br />
</div> <!-- close top-section--><br />
<div id="content"><br />
<a name="top" id="top"></a><br />
<h1 class="firstHeading">Team:Sumbawagen/Team</h1><br />
<div id="bodyContent"><br />
<h3 id="siteSub" class='noprint'>From 2014.igem.org</h3><br />
<div id="contentSub"></div><br />
<!--<br />
<div id="jump-to-nav">Jump to: <a href="#column-one">navigation</a>, <a href="#searchInput">search</a></div>--><br />
<!-- start content --><br />
<p><br />
<html lang="en"><br />
<head><br />
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" /><br />
<br />
<script type="text/javascript" src="swfobject.js"></script><br />
<script type="text/javascript"><br />
swfobject.registerObject("myFlashContent", "9.0.0", "expressInstall.swf");<br />
</script><br />
<title>iGEM Sumbawagen 2014 &middot; Econey</title><br />
<meta name="viewport" content="width=device-width, initial-scale=1.0"><br />
<meta name="description" content="A synthetic biology project called Econey from the Sumbawagen iGEM Team."><br />
<meta name="author" content="Sumbawagen Team 2014"><br />
<br />
<!-- CSS --><br />
<link href=' http://fonts.googleapis.com/css?family=Open+Sans' rel='stylesheet' type='text/css'><br />
<!-- <link href="http://www.kyleharrison.co.uk/igem/assets/css/bootstrap.css" rel="stylesheet"><br />
<br />
<link href="http://www.kyleharrison.co.uk/igem/assets/css/style.css" rel="stylesheet"> --><br />
<br />
<link href="https://2013.igem.org/Team:Dundee/stylesheet?action=raw&amp;ctype=text/css" rel="stylesheet"><br />
<link href="https://2013.igem.org/Team:Dundee/bootstrap?action=raw&amp;ctype=text/css" rel="stylesheet"><br />
<br />
<!--<br />
<link href="http://www.kyleharrison.co.uk/igem/assets/css/bootstrap-responsive.css" rel="stylesheet"><br />
--><br />
<!-- HTML5 shim, for IE6-8 support of HTML5 elements --><br />
<!--[if lt IE 9]><br />
<script src="http://www.kyleharrison.co.uk/igem/assets/js/html5shiv.js"></script><br />
<![endif]--><br />
<br />
<!-- Fav and touch icons --><br />
<link rel="apple-touch-icon-precomposed" sizes="144x144" href="../assets/ico/apple-touch-icon-144-precomposed.png"><br />
<link rel="apple-touch-icon-precomposed" sizes="114x114" href="../assets/ico/apple-touch-icon-114-precomposed.png"><br />
<link rel="apple-touch-icon-precomposed" sizes="72x72" href="../assets/ico/apple-touch-icon-72-precomposed.png"><br />
<link rel="apple-touch-icon-precomposed" href="../assets/ico/apple-touch-icon-57-precomposed.png"><br />
<link rel="shortcut icon" href="../assets/ico/favicon.png"><br />
</head><br />
<body><br />
<br />
<!-- Part 1: Wrap all page content here --><br />
<div id="wrap"><br />
<br />
<!-- Fixed navbar --><br />
<div class="navbar navbar-fixed-top"><br />
<div class="navbar-inner"><br />
<br />
<div class="container" style="width:1024px"><br />
<button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse"><br />
<span class="icon-bar"></span><br />
<span class="icon-bar"></span><br />
<span class="icon-bar"></span><br />
</button><br />
<a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="https://igem.org"><img width="70px" height="50px" src="https://static.igem.org/mediawiki/2013/1/17/IGEM_basic_Logo_white_stylized.png"> </a><br />
<a class="brand" href="https://2014.igem.org/Team:Sumbawagen/Parts">Sumbawagen</a><br />
<br />
<div class="nav-collapse collapse"><br />
<ul class="nav"><br />
<li class="active"><a href="https://2014.igem.org/Team:Sumbawagen">Home</a></li><br />
<br />
<br />
<li class="dropdown"><br />
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey"> E-coney Project </a></li><br />
</ul><br />
</li><br />
<br />
<br />
<br />
<li class="dropdown"><br />
<a href="https://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li> <br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li> <br />
<br />
<br />
<br />
</ul><br />
<br />
</li><br />
<br />
<li class="dropdown"><br />
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook">Daily Notes</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li><br />
<!--<li><a href="#">Improvements</a></li>--><br />
</ul><br />
</li><br />
<br />
<br />
<br />
<li class="dropdown"><br />
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Team <b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li><br />
<li><a href="http:https://igem.org/Team.cgi">Team Information</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li><br />
<li class="divider"></li><br />
<li class="nav-header">Social Media</li><br />
<li><a href="https://www.facebook.com/sumbawagen2014?fref=ts">Facebook</a></li><br />
<li><a href="http://sumbawagen.blogspot.com/">Blog</a></li><br />
</ul><br />
</li><br />
<br />
<li class="dropdown"><br />
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<br />
<br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Policy"><br />
<strong>Policy & Practices</strong><br />
<div style=" <br />
font-size: 100%;<br />
font-weight: bold;<br />
line-height: 1;<br />
color: rgb(66, 139, 202);<br />
text-align: center;<br />
white-space: nowrap;<br />
vertical-align: baseline;<br />
display: inline;"><br />
</div><br />
<br />
</a></li><br />
<li class="divider"></li> <br />
<br />
<br />
</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responds and supports</a></li><br />
<br />
<br />
</ul><br />
</li><br />
<br />
<br />
<li class="dropdown"><br />
<a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a><br />
<ul class="dropdown-menu"><br />
<li><a href="https://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li><br />
</ul><br />
</li><br />
<br />
<br />
<br />
<br />
</ul><br />
</div><!--/.nav-collapse --><br />
</div><br />
</div><br />
</div><br />
<br />
<br />
</p><p><br />
<html lang="en"><br />
<br />
<!-- Begin page content --><br />
<div class="container"><br />
<br />
<br />
<div class="row" style="text-align:justify;margin-top:-20px;"><br />
<div class="span12"><br />
<br />
<h2>Notebook – Protocol – 13. SDS-PAGE</h2><br />
<ul style="padding-left:25px;"><br />
<p>1. 1 ml of E. coli BL21(DE3) overnight culture was added to 1.5 ml propylene tube. Then centrifuge at 6,000 rpm, 5 minutes at room temperature. Then supernatant was discarded.</p><br />
<p>2. Pellet was added with 300 ul of 1x Bug Buster (Novagen). Tube was vortexed.</p><br />
<p>3. Tube was put on boiled water for 5 minutes.</p><br />
<p>4. 50 ul of samples was aliquoted and used as “total protein” sample.</p><br />
<p>5. The rest of the sample in the tube was centrifuged at 6,000 rpm, 10 min, room temperature.</p><br />
<p>6. 50 ul of supernatant was aliquoted and used as “soluble protein’ sample.</p><br />
<p>7. Protein concentration of “total protein” and “soluble protein” were estimated using Lowry method based on the kit from Biorad. We have no UV spectrophotometer for measurement of real concentration at 750 nm. But according to our chief instructor Dr. Arief previous experiment, the concentration of “soluble protein” will be about 1,000 – 2,000 ug/ml, while the concentration of “total protein” will be about 10,000 – 20,000 ug/ml.</p><br />
<p>8. “Soluble protein” sample was used directly, while “total protein” sample was diluted 1/10th to obtain samples with roughly the same concentration for SDS-PAGE.</p><br />
<p>9. 15 ul sample was added with 15 ul dye containing SDS and Coomassie Brilliant Blue (CBB) in 500 ul polypropylene tube, then the tube was put in boiled water for 15 min</p><br />
<p>10. 15 ul of sample – protein quantity about 10 ug – was electrophoresed. Marker used was Blue Star Prestained Protein Marker (Nippon Genetics). The marker has proteins bands of 170 / 125 / 93 / 68 / 53 / 41 / 32 / 23 / 14 / 10 kD.</p><br />
<p>11. SDSPAGE was done with commercial 10-20% SDS gel, and 1x SDS buffer. 10x SDS buffer was prepared using 250 mM Tris, 1,920 mM Glycine, and 1% w/v SDS.</p><br />
<p>12. SDSPAGE was done for 45 minutes with equipment setting of 40 mA.</p><br />
<p>13. Staining of SDSPAGE gel was done using Rapid CBB kit (Kanto Chemicals). Staining was done 1-3 hours.</p><br />
<p>14. Destaining of SDSPAGE gel was done using solution containing 75 ml distilled water, 10 ml acetic acid, 15 ml ethanol. Destaining was done overnight.</p><br />
<p>15. Gel was documented with pocket camera, and file was evaluated with Photoshop program in a computer.</p><br />
</p></ul><br />
<br />
<br />
<!-- Title --><br />
<div class="page-header"><br />
<center><h2><b><strong>Sponsors</strong></b> </h2></center><br />
<center><img src=" https://static.igem.org/mediawiki/2014/8/8b/All_sponsor.png"style="width:900px;height:260px;"/><br></center><br />
<br />
</div><br />
<!-- Title End --><br />
<br />
</div><br />
</i></em><br />
<br />
<br><br><br />
<br />
<br />
<br />
</div><!-- Row End --><br />
<br />
<br />
<div id="push"></div><br />
</div><br />
<br />
<br />
<br />
<!-- Le javascript<br />
================================================== --><br />
<!-- Placed at the end of the document so the pages load faster --><br />
<br />
<!-- the neccessary javascript --><br />
<script src="http://code.jquery.com/jquery-latest.js"></script><br />
<script src="http://www.kyleharrison.co.uk/igem/js/bootstrap.min.js"></script><br />
<br />
<br />
</body><br />
<br />
</p><br />
<!-- <br />
NewPP limit report<br />
Preprocessor node count: 9/1000000<br />
Post-expand include size: 41/2097152 bytes<br />
Template argument size: 0/2097152 bytes<br />
Expensive parser function count: 0/100<br />
--><br />
<br />
<!-- Saved in parser cache with key 2013_igem_org:pcache:idhash:35709-0!1!0!!en!2 and timestamp 20140902155529 --><br />
<div class="printfooter"><br />
Retrieved from "<a href="https://2013.igem.org/Team:Dundee/Project/MopQuantifying">https://2013.igem.org/Team:Dundee/Project/MopQuantifying</a>"</div><br />
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content --><br />
<div class="visualClear"></div><br />
</div><br />
</div><br />
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR --><br />
<div class="visualClear"></div><br />
<div id='footer-box' class='noprint'><br />
<div id="footer"><br />
<div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div> <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div> <ul id="f-list"><br />
<br />
<br />
<!-- Recentchanges is not handles well DEBUG --><br />
<li id="t-recentchanges"><a href="/Special:RecentChanges"<br />
title='Recent changes'>Recent changes</a></li><br />
<br />
<li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Dundee/Team"<br />
title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li><br />
<br />
<li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Dundee/Team"<br />
title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li><br />
<br />
<br />
<br />
<li id="t-specialpages"><a href="/Special:SpecialPages"<br />
title="List of all special pages [q]" accesskey="q">Special pages</a><br />
</li><br />
<li><a href='/Special:Preferences'>My preferences</a></li><br />
</ul><br />
</div> <!-- close footer --><br />
<div id='footer'><br />
<ul id="f-list"><br />
<br />
<li id="t-print"><a href="/wiki/index.php?title=Team:Dundee/Team&amp;printable=yes"<br />
title="Printable version of this page [p]" accesskey="p">Printable version</a><br />
</li><br />
<br />
<li id="t-permalink"><a href="/wiki/index.php?title=Team:Dundee/Team&amp;oldid=325506"<br />
title="Permanent link to this revision of the page">Permanent link</a><br />
</li><br />
<br />
<br />
<li id="privacy"><a href="/2013.igem.org:Privacy_policy" title="2013.igem.org:Privacy policy">Privacy policy</a></li><br />
<li id="disclaimer"><a href="/2013.igem.org:General_disclaimer" title="2013.igem.org:General disclaimer">Disclaimers</a></li><br />
</ul><br />
</div> <!-- close footer --><br />
</div> <!-- close footer-box --> <br />
<br />
<script>if (window.runOnloadHook) runOnloadHook();</script><br />
</div><br />
<!-- Served in 0.248 secs. --></body><br />
<br />
</html></div>
Adel