Team:Sumbawagen/Notebook/protocol12

From 2014.igem.org

(Difference between revisions)
Line 171: Line 171:
             </button>
             </button>
<a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="http://igem.org"><img width="70px" height="50px" src="http://2013.igem.org/wiki/images/1/17/IGEM_basic_Logo_white_stylized.png"> </a>
<a class="brand" style="padding:0px 15px;width:70px;height:50px;" href="http://igem.org"><img width="70px" height="50px" src="http://2013.igem.org/wiki/images/1/17/IGEM_basic_Logo_white_stylized.png"> </a>
-
             <a class="brand" href="http://2014.igem.org/Team:Sumbawagen/Parts">Sumbawagen</a>
+
             <a class="brand" href="http://2014.igem.org/Team:Sumbawagen">Sumbawagen</a>
             <div class="nav-collapse collapse">
             <div class="nav-collapse collapse">
Line 181: Line 181:
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                       <li><a href="http://2014.igem.org/Team:Sumbawagen/overviews/Econey"> E-coney Project </a></li>
+
                       <li><a href="http://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
                     </ul>
                     </ul>
                 </li>
                 </li>
Line 196: Line 196:
<li><a href="http://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
<li><a href="http://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
<li><a href="http://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>  
<li><a href="http://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>  
-
<li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>                          
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
                                
                                
                    
                    
Line 207: Line 208:
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Daily Notes</a></li>
+
 
 +
                    <li class="nav-header">Daily Notes</li>
 +
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
 +
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
 +
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook3">Mobile Programming</a></li>
 +
                  <li class="divider"></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <!--<li><a href="#">Improvements</a></li>-->
                     <!--<li><a href="#">Improvements</a></li>-->
Line 219: Line 225:
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
-
                    <li><a href="http:http://igem.org/Team.cgi">Team Information</a></li>
 
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
Line 259: Line 264:
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
-
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responds and supports</a></li>
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
                                                            
                                                            

Revision as of 03:05, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 12. Honey glucose meter measurement

    1. Glucose dissolved in distilled water to a concentrations of 100 mM was used as stock. From that stock, various concentration of glucose were prepared by dilution. The concentrations were 5 / 10 / 15 / 20 / 25 mM.

    2. Those glucose stocks were measured its mg/dl value by a glucose meter. The values then plotted as y axis, against the concentration as x axis. Linear standard curve was obtained and the formula was used to calculate the concentration of unknown sample.

    3. Honey in 1.5 ml polypropylene tube was warmed at 60 oC for 10 minutes, then transferred 100 ul to a new tube which contained 900 ul distilled water to obtain 1/10 dilution.

    4. This sample was used for measurement of glucose concentration by glucose meter, and its glucose concentration was calculated using standard curve obtained before.

    5. When the mg/dl value still exceeded the linear curve, dilution rate was increased.