Team:Sumbawagen/Notebook/protocol11

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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
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                       <li><a href="https://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
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                    <li class="nav-header">Daily Notes</li>
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                     <li><a href="https://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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<li><a href="https://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<h2>Notebook – Protocol – 3. Electrophoresis and DNA visualization</h2>
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<h2>Notebook – Protocol – 11. Cultivation</h2>
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<p>2. The pre-culture was shaken over-night at room temperature.</p>  
<p>2. The pre-culture was shaken over-night at room temperature.</p>  
<p>3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.</p>
<p>3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.</p>
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<p>4. 4. Main culture was shaken over-night at room temperature. </p>
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<p>4. Main culture was shaken over-night at room temperature. </p>
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Latest revision as of 09:26, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 11. Cultivation

1. From plate of E. coli BL21/DE3 harboring appropriate plasmid, colony was picked and cultured in 2 ml LB medium containing antibiotic chloramphenicol (Cam) with final concentration of 34 ug/ml. 10 ml size glass tube was used. This was pre-culture.

2. The pre-culture was shaken over-night at room temperature.

3. Main culture was prepared for the next day, which contains pre-culture, LB medium, Cam, expression reagent for the expression of reporter gene mRFP (autoinduction medium which includes 20x NPS, 50x 5052 and 1,000x Mg), sugar for catabolite repression induction (either glucose, fructose, glucose+fructose, or honey). Detail receipt with various concentration of sugars was given in Tables below.

4. Main culture was shaken over-night at room temperature.