Team:Sumbawagen/Notebook/protocol10

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<li><a href="http://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>  
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>                          
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
                                
                                
                    
                    
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
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                    <li class="nav-header">Daily Notes</li>
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                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
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                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
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                    <li><a href="http:http://igem.org/Team.cgi">Team Information</a></li>
 
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responds and supports</a></li>
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<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
                                                            
                                                            

Latest revision as of 09:25, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Notebook – Protocol – 10. DNA Sequencing

DNA sequencing was done by ordering to commercial company.

1. Premix sample was prepared with the following composition:

  • Plasmid sample, 10 to 20 ul*
  • 10 uM primer, 1 ul
  • Sterilized distilled water, 9 to 19 ul
  • Total volume, 21 ul
    Concentration < 900 ng

2. Samples were sent to the company

3. According to the company, DNA sequencing was done using the following conditions:

  • Sequencing kit, BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems)
  • Purification kit, BigDye XTerminator® Purification Kit (Applied Biosystems)
  • Sequencer, Applied Biosystems 3730xl DNA Analyzer

4. DNA sequencing data in .ab1 files were received by email, then analyzed using Bioedit program. For alignment, online CLUSTAL program was used.