Team:Sumbawagen/Notebook

From 2014.igem.org

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          <h2><b>Week 1</b> </h2>
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<h2><b>POLICY AND PRACTICE</b> </h2>
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<h3><b>Part 1 Survey to Society</b> </h3><br>
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<p>We began our project by preparing material needed. We made liquid and solid medium using LB powder. We also made solution for transformation process like MgCl2, and TSS medium. In this week, we also culture the stock of our RFP in liquid medium. We aim to make the stock of RFP and also examine whether the RFP still work or not. The culture of RFP is not growth. It indicates that our RFP stock has been damaged due to the unstable storage temperature. </p>
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<ul style="padding-left:25px;">
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<p>1. 1 - 4th July, 2014</p>
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<p>Discussion of Human Practice (now Policy & Practice) project plan </p>
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<p>2. 8 - 11th July, 2014</p>
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<p>Permission confirmation to the district and village office to do the survey for the society</p>
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<p>3. 14 - 17th July, 2014</p>
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<p>Data collection (survey)</p>
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<p>4. 21th July, 2014</p>
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<p>Data analysis </p>
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<p>5. 22 - 26th July, 2014</p>
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<p>Result discussion</p>
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</p></ul>
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<strong>Detail Activity: </strong>
 
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<p>1.August 14 th, 2014</p>
 
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Making liquid medium using LB medium powder, sterilization using autoclave, add antibiotic, culture E coli DH5α, shaking
 
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<p>2.August 15th, 2014</p>
 
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Making solution MgSO4, glucose 1 M, CaCl2, TSS medium
 
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<p>3.August 16th, 2014</p>
 
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Making solid, liquid, and NA medium, Culture E coli (Glycerol stock RFP)
 
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<p>4.August 17th, 2014</p>
 
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Add chlorampenichol to solid medium (LB medium), streak RFP (stock 6 tubes), check culture E coli (Glycerol stock RFP)
 
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<h2>Week 2</h2>
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<h3><b>Part 2 Demo of Our Laboratory Activity</b> </h3><br>
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<p>In the second week we culture the stock of RFP into the solid medium. The purpose is to make the stock of RFP in the solid medium. We also transformed the plasmid Bba_J04450 and Bba_B0034 into E coli BL21 (DE3). We did transformation twice in this week. The first transformation does not show any red colony growing. But the second transformation showed red colonies growing indicates that the transformation process success.<br><br>
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<ul style="padding-left:25px;">
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<strong>Detail Activity : </strong>
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<p>6. 7-8th September, 2014</p>
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<p>1. August 18th, 2014</p>
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<p>#Permission to village office for Policy and Practice activity in Batu Dulang village, Batu Lanteh District and Gapit Village, Sumbawa Regency
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Making solid medium with chlorampenicol, take Bba_B0034 from kit plate and storage
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#Analysis the last laboratory result
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<p>2. August 19th, 2014</p>
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Making ampicilin stock 1 M, streak RFP (stock lab) medium padat, transformation
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<p>3. August 20th, 2014</p>
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Check Bba_J04450 transformation result, culture RFP, streak RFP, making ampicilin stock 1 M, plating transformation result, streak RFP from solid medium
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<p>4. August 21th, 2014</p>
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Check transformation results (Not growing), check RFP streak (contaminant), streak transformation result
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<p>5. August 22th, 2014</p>
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Culture E coli glycerol stock (BL21(DE3)), Transformation
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</p>
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<p>7. 9-10th September, 2014</p>
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<p>Policy and Practice activity in Batu Dulang village</p>
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<p>8. 11-12th September, 2014</p>
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<p>Policy and Practice activity in Gapit village</p>
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<p>9. 13-15th September, 2014</p>
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<p>Edit video Policy and Practice activity in Batu Dulang and Gapit village </p>
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</p></ul>
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<h3><b>Part 3 Taking Video about Societies Opinion about Biotechnology</b> </h3><br>
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<p>10. 16th September, 2014</p>
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<p>Permission to some government office for take video about the opinion of public policy officer and head of religion community in Sumbawa Regency</p>
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<p>11. 17-19th September, 2014</p>
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<p>Take video about the opinion of high public policy officer and head of religion community in Sumbawa Regency</p>
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          <h2>Week 3</h2>
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<p>12. 20-21th September, 2014</p>
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          <P><strong> Detail Activity </strong></p>
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<p>Edit video about the opinion of public policy officer and head of religion community in Sumbawa Regency</p>
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<p>1. 25 August, 2014</p>
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</p></ul>
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Culture RFP DH5α (Add over expression reagent), making chlorampenicol stock
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<p>2. 26 August, 2014</p>
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Culture E coli glycerol stock RFP, culture over expression DH5α in 6 tube, streak RFP from single colony and line colony to the LB medium with and without chlorampenicol
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<p>3. 27 August, 2014</p>
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Plating transformation results, streak stock RFP
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          <center><strong><h2><b>Sponsors</b> </h2></strong></center>
 
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<center><img src="http://2014.igem.org/wiki/images/3/30/Logo_Alzyme.jpg"style="width:250px;height:70px;"/><br></center>
 
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<center><img src="http://2014.igem.org/wiki/images/1/17/Logo-UTS.png"><br></center>
 
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Revision as of 03:50, 18 October 2014

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Part 1 Survey to Society


    1. 1 - 4th July, 2014

    Discussion of Human Practice (now Policy & Practice) project plan

    2. 8 - 11th July, 2014

    Permission confirmation to the district and village office to do the survey for the society

    3. 14 - 17th July, 2014

    Data collection (survey)

    4. 21th July, 2014

    Data analysis

    5. 22 - 26th July, 2014

    Result discussion

Part 2 Demo of Our Laboratory Activity


    6. 7-8th September, 2014

    #Permission to village office for Policy and Practice activity in Batu Dulang village, Batu Lanteh District and Gapit Village, Sumbawa Regency #Analysis the last laboratory result

    7. 9-10th September, 2014

    Policy and Practice activity in Batu Dulang village

    8. 11-12th September, 2014

    Policy and Practice activity in Gapit village

    9. 13-15th September, 2014

    Edit video Policy and Practice activity in Batu Dulang and Gapit village

Part 3 Taking Video about Societies Opinion about Biotechnology


    10. 16th September, 2014

    Permission to some government office for take video about the opinion of public policy officer and head of religion community in Sumbawa Regency

    11. 17-19th September, 2014

    Take video about the opinion of high public policy officer and head of religion community in Sumbawa Regency

    12. 20-21th September, 2014

    Edit video about the opinion of public policy officer and head of religion community in Sumbawa Regency