Team:Sumbawagen

From 2014.igem.org

(Difference between revisions)
 
(45 intermediate revisions not shown)
Line 1: Line 1:
-
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
+
<html xlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<head>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
Line 10: Line 10:
<style type="text/css" media="screen, projection">/*<![CDATA[*/
<style type="text/css" media="screen, projection">/*<![CDATA[*/
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/common/shared.css?270";
-
@import "/wiki/skins/igem/main.css?270";
+
@import "/wiki/skins/igem/main.cs70";
/*]]>*/</style>
/*]]>*/</style>
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
Line 181: Line 181:
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Overviews <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                       <li><a href="http://2014.igem.org/Team:Sumbawagen/overviews/Project"> Project </a></li>
+
                       <li><a href="http://2014.igem.org/Team:Sumbawagen/overviews/Econey_Project"> Econey Project </a></li>
-
                      <!--<li><a href="#">Sub Menu 2 </a></li>-->
+
                     </ul>
-
                      <li><a href="http://2014.igem.org/Team:Sumbawagen/Modeling">Modelling </a></li>
+
-
                      <li><a href="http://2014.igem.org/Team:Sumbawagen/overviews/Our_Software">Our Software</a></li>
+
-
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/humanpractice_overview">Human Practice </a></li>
+
-
                    <!--<li><a href="#">Sub Menu 5</a></li>-->
+
-
                  </ul>
+
                 </li>
                 </li>
Line 195: Line 190:
                   <a href="http://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
                   <a href="http://2014.igem.org/Team:Sumbawagen/Team" class="dropdown-toggle" data-toggle="dropdown">Project <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                    <li class="nav-header">Lab</li>
+
                 
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/project/econey">Econey</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/project/result">Result</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/project/software">Software</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/parts">Parts</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attribution</a></li>
 +
<li><a href="http://2014.igem.org/Team:Sumbawagen/future_direction">Future Direction</a></li>                             
 +
                             
 +
                 
 +
                   
 +
                  </ul>
-
                  <!-- <li><a href="#">Mop</a></li>-->
 
-
<li><a href="http://2014.igem.org/Team:Sumbawagen/project/lab">Biotechnology</a></li>
 
-
<li><a href="#">Sub Menu 12</a></li>
 
-
<li><a href="#">Sub Menu 13</a></li>
 
-
                    <li><a href="#">Sub Menu 14 </a></li>
 
-
                    <li><a href="#">Sub Menu <i>Miring</i> </a></li>
 
-
                    <li class="divider"></li>
 
-
                    <li class="nav-header">Our Software </li>
 
-
 
-
                    <!--<li><a href="#">Sub Menu 21</a></li>-->
 
-
                    <li><a href="#">Sub Menu 22</a></li>
 
-
                    <li><a href="#">Sub Menu 23</a></li>
 
-
                    <li><a href="#">Sub Menu 24 </a></li>
 
-
                    <li><a href="#">Sub Menu 25</a></li>
 
-
 
-
                    <li class="divider"></li>
 
-
                    <li class="nav-header">Software &amp; Hardware</li>
 
-
                  <li><a href="#">Glucose Measurement</a></li>
 
-
                  <li><a href="#">Android Application</a></li>
 
-
                  <li><a href="#">Sub Menu 31 </a></li>
 
-
 
-
                    <li class="divider"></li>
 
-
                    <li class="nav-header">Attributions </li>
 
-
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Attributions">Attributions </a></li>
 
-
                  </ul>
 
                 </li>
                 </li>
                 <li class="dropdown">
                 <li class="dropdown">
-
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Parts <b class="caret"></b></a>
+
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Notebook <b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="#">Our Biobricks</a></li>
+
 
 +
                    <li class="nav-header">Daily Notes</li>
 +
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Policy & Practices</a></li>
 +
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook2">Lab</a></li>
 +
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook/Protocol">Protocol</a></li>
                     <!--<li><a href="#">Improvements</a></li>-->
                     <!--<li><a href="#">Improvements</a></li>-->
                   </ul>
                   </ul>
                 </li>
                 </li>
-
                 <li><a href="http://2014.igem.org/Team:Sumbawagen/Safety">Safety</a></li>
+
                  
  <li class="dropdown">
  <li class="dropdown">
Line 238: Line 223:
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team">Meet the Team</a></li>
-
                    <li><a href="http://igem.org/Team.cgi">Team Information</a></li>
 
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Gallery">Gallery</a></li>
-
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/Notebook">Notebook</a></li>
 
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Team/Contact">Contact</a></li>
                     <li class="divider"></li>
                     <li class="divider"></li>
Line 250: Line 233:
                 <li class="dropdown">
                 <li class="dropdown">
-
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Human Practice <!--<sup>*new</sup>--><b class="caret"></b></a>
+
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown">Policy & Practices<!--<sup>*new</sup>--><b class="caret"></b></a>
                   <ul class="dropdown-menu">
                   <ul class="dropdown-menu">
-
                     <li><a href="# ">
+
                   
-
Sub Menu 41
+
                     <li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Policy">
 +
<strong>Policy & Practices</strong>
<div style="   
<div style="   
     font-size: 100%;
     font-size: 100%;
Line 267: Line 251:
</a></li>
</a></li>
 +
<li class="divider"></li> 
 +
     
-
                    <li><a href="# ">
+
</a></li>
-
Sub Menu 42
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/survey">Survey</a></li>
-
<div style="  
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Biotech">Biotech Camp</a></li>
-
    font-size: 75%;
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/meet_the">Meet the farmers</a></li>
-
    font-size: 100%;
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/meet">Meet US Ambassador</a></li>
-
    font-weight: bold;
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/econey">@america</a></li>
-
    line-height: 1;
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/moyo">Moyo Festival</a></li>
-
    color: rgb(66, 139, 202);
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/virtual">Virtual Outreach</a></li>
-
    text-align: center;
+
<li><a href="http://2014.igem.org/Team:Sumbawagen/Human_practice/Support">Responses and supports</a></li>
-
    white-space: nowrap;
+
 
-
     vertical-align: baseline;
+
                                                         
-
     display: inline;">
+
                  </ul>
-
  <sup>*New</sup></div>
+
                </li>
 +
 
 +
               
 +
                <li class="dropdown">
 +
                  <a href="#" class="dropdown-toggle" data-toggle="dropdown">Interlab Study <b class="caret"></b></a>
 +
                  <ul class="dropdown-menu">
 +
                    <li><a href="http://2014.igem.org/Team:Sumbawagen/interlabstudy/results">Results</a></li>
 +
                  </ul>
 +
                </li>
 +
 
 +
 
 +
             
 +
 
 +
              </ul>
 +
            </div><!--/.nav-collapse -->
 +
          </div>
 +
        </div>
 +
      </div>
 +
 
 +
 
 +
</p><p>
 +
<html lang="en">
 +
 
 +
<!-- Begin page content -->
 +
<div class="container">
 +
 
 +
      <div class="span12" style="margin-left:0px;margin-top: 10px;">
 +
 
 +
            <!-- a very basic slider, note the structure of each item. you can add too but not take away (classes and id's that is) -->
 +
            <div id="slider" class="carousel slide">
 +
 
 +
              <div class="carousel-inner">
 +
                <div class="item active"><!-- add the active class to any slider you want shown first -->
 +
                  <img src="http://2014.igem.org/wiki/images/7/7e/Sumbawa_baner.png" style="width:100%;height:100%">
 +
                </div>
 +
               
 +
                <div class="item"><!-- add the active class to any slider you want shown first -->
 +
                  <img src="http://2014.igem.org/wiki/images/a/af/Banner_SBG3.jpg" style="width:100%;height:100%">
 +
                </div>
 +
              </div>
 +
 
 +
              <!-- the controls for our sliders -->
 +
              <a class="left carousel-control " href="#slider" data-slide="prev">&lsaquo;</a>
 +
              <a class="right carousel-control " href="#slider" data-slide="next">&rsaquo;</a>
 +
 
 +
            </div>
 +
      </div>
 +
 
 +
      <div class="row">
 +
      <!-- the main content -->
 +
 
 +
        <div class="span6" style="text-align:justify">
 +
        <h2 style="margin-top:-10px;"> Project Description </h2>
 +
 
 +
        <p>
 +
        BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.<br><br></p>
 +
 
 +
 
 +
        <br>
 +
        </div>
 +
 
 +
        <div class="span6" style="padding-top:40px" >
 +
              <iframe width="460" height="315" src="//www.youtube.com/embed/XICTHe_YXS8" frameborder="0" allowfullscreen></iframe>
 +
          </div>
 +
      </div><!-- Row End -->
 +
 
 +
 
 +
<!--
 +
      <div class="row" style="padding-bottom:20px">
 +
      <div class="span6">text</div>
 +
      <div class="span6"><img src="#"></div>
 +
      </div>
 +
-->
 +
 
 +
 
 +
      <!-- Row Three -->
 +
      <div class="row">
 +
 
 +
      <div class="span3" style="height:275px">
 +
        <div id="mainwrapper">
 +
        <a href="http://2014.igem.org/Team:Sumbawagen">
 +
                    <!-- Image Caption 6 -->
 +
              <div id="box-6" class="box">
 +
                <img id="image-6" src="http://2014.igem.org/wiki/images/b/b8/Home_SBG.jpg" style="width:220px;height:220px;"/>
 +
              <span class="caption scale-caption" style="text-align:justify">
 +
                          <p><b style="font-size:16px">Home</b><br><br> </p>
 +
                </span>
 +
              </div>
 +
            </div> </a>
 +
      </div>
 +
 
 +
      <div class="span3" style="height:275px">
 +
          <div id="mainwrapper">
 +
        <a href="http://2014.igem.org/Team:Sumbawagen/HumanPractice">
 +
                  <!-- Image Caption 6 -->
 +
            <div id="box-6" class="box">
 +
              <img id="image-6" src="http://2014.igem.org/wiki/images/b/b0/Humanpractice_SBG.png" style="width:220px;height:220px;"/>
 +
              <span class="caption scale-caption" style="text-align:justify">
 +
                          <p><b style="font-size:16px;">Policy & Practices </b><br><br> </p>
 +
              </span>
 +
            </div>
 +
          </div></a>
 +
        </div>
 +
 
 +
      <div class="span3" style="height:275px">
 +
        <div id="mainwrapper">
 +
        <a href="http://2014.igem.org/Team:Sumbawagen/Team">
 +
                  <!-- Image Caption 6 -->
 +
            <div id="box-6" class="box">
 +
              <img id="image-6" src="http://2014.igem.org/wiki/images/f/fa/Team_SBG.jpg" style="width:220px;height:220px;"/>
 +
              <span class="caption scale-caption" style="text-align:justify">
 +
                          <p><b style="font-size:16px;">Team</b><br><br>  </p>
 +
              </span>
 +
            </div>
 +
          </div></a>
 +
        </div>
 +
 
 +
      <div class="span3" style="height:275px">
 +
          <div id="mainwrapper">
 +
        <a href="http://2014.igem.org/Team:Sumbawagen/Project">
 +
                  <!-- Image Caption 6 -->
 +
            <div id="box-6" class="box">
 +
              <img id="image-6" src="http://2014.igem.org/wiki/images/8/87/Econey_SBG.jpg" style="width:220px;height:220px;"/>
 +
              <span class="caption scale-caption" style="text-align:justify">
 +
 
 +
                          <p><b style="font-size:16px;">ECONEY Project </b><br><br></p>
 +
 
 +
</span>
 +
            </div>
 +
          </div></a>
 +
        </div>
 +
      </div>
 +
 
 +
<!-- Title -->
 +
      <div class="page-header">
 +
          <center><h2><b><strong>Acknowledgment<strong></b> </h2></center>
 +
<center><img src="http://2014.igem.org/wiki/images/7/75/BLKM.png"style="width:145px;height:225px;"/><br></center>
 +
 
 +
        </div>
 +
      <!-- Title End -->
 +
 
 +
<!-- Title -->
 +
      <div class="page-header">
 +
          <center><h2><b><strong>Sponsors</strong></b> </h2></center>
 +
<center><img src="http://2014.igem.org/wiki/images/8/8b/All_sponsor.png"style="width:900px;height:260px;"/><br></center>
 +
      </div><!-- End Page Content -->
 +
 
 +
      <!-- Le javascript
 +
     ================================================== -->
 +
    <!-- Placed at the end of the document so the pages load faster -->
 +
 
 +
    <!-- the neccessary javascript -->
 +
    <script src="http://code.jquery.com/jquery-latest.js"></script>
 +
    <script src="http://www.kyleharrison.co.uk/igem/js/bootstrap.min.js"></script>
 +
 
 +
 
 +
  </body>
 +
 
 +
</p><p><br />
 +
</p>
 +
<!--
 +
NewPP limit report
 +
Preprocessor node count: 11/1000000
 +
Post-expand include size: 41/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Expensive parser function count: 0/100
 +
-->
 +
 
 +
<!-- Saved in parser cache with key 2013_igem_org:pcache:idhash:239-0!1!0!!en!2!edit=0 and timestamp 20140814173856 -->
 +
<div class="printfooter">
 +
Retrieved from "<a href="http://2013.igem.org/Team:Dundee">http://2013.igem.org/Team:Dundee</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 +
<div class="visualClear"></div>
 +
    <div id='footer-box' class='noprint'>
 +
        <div id="footer">
 +
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 +
 +
 
 +
  <!-- Recentchanges is not handles well DEBUG -->
 +
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 +
      title='Recent changes'>Recent changes</a></li>
 +
 
 +
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Dundee"
 +
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 +
 
 +
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Dundee"
 +
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 +
 
 +
 
 +
 
 +
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 +
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
                </li>
 +
                <li><a href='/Special:Preferences'>My preferences</a></li>
 +
            </ul>
 +
        </div> <!-- close footer -->
 +
        <div id='footer'>
 +
    <ul id="f-list">
 +
 
 +
            <li id="t-print"><a href="/wiki/index.php?title=Team:Dundee&amp;printable=yes"
 +
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 
 +
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Dundee&amp;oldid=354262"
 +
              title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
 +
 
 +
 
 +
        <li id="privacy"><a href="/2013.igem.org:Privacy_policy" title="2013.igem.org:Privacy policy">Privacy policy</a></li>
 +
        <li id="disclaimer"><a href="/2013.igem.org:General_disclaimer" title="2013.igem.org:General disclaimer">Disclaimers</a></li>
 +
    </ul>
 +
</div> <!-- close footer -->
 +
     </div> <!-- close footer-box -->
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
<!-- Served in 0.167 secs. --></body>
 +
 
 +
 
 +
</p>
 +
<!--
 +
NewPP limit report
 +
Preprocessor node count: 4/1000000
 +
Post-expand include size: 0/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Expensive parser function count: 0/100
 +
-->
 +
 
 +
<!-- Saved in parser cache with key 2014_igem_org:pcache:idhash:1032-0!1!0!!en!2 and timestamp 20141016110802 -->
 +
<div class="printfooter">
 +
Retrieved from "<a href="http://2014.igem.org/Team:Sumbawagen">http://2014.igem.org/Team:Sumbawagen</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE  -- RDR  -->
 +
<div class="visualClear"></div>
 +
    <div id='footer-box' class='noprint'>
 +
        <div id="footer">
 +
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
 +
 +
 
 +
  <!-- Recentchanges is not handles well DEBUG -->
 +
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
 +
      title='Recent changes'>Recent changes</a></li>
 +
 
 +
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Sumbawagen"
 +
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
 +
 
 +
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Sumbawagen"
 +
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
 +
 
 +
 
 +
 
 +
                <li id="t-upload"><a href="/Special:Upload"
 +
                  title="Upload files [u]" accesskey="u">Upload file</a>
 +
                </li>
 +
                <li id="t-specialpages"><a href="/Special:SpecialPages"
 +
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
 +
                </li>
 +
                <li><a href='/Special:Preferences'>My preferences</a></li>
 +
            </ul>
 +
        </div> <!-- close footer -->
 +
        <div id='footer'>
 +
    <ul id="f-list">
 +
 
 +
            <li id="t-print"><a href="/wiki/index.php?title=Team:Sumbawagen&amp;printable=yes"
 +
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
 +
            </li>
 +
 
 +
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Sumbawagen&amp;oldid=269751"
 +
              title="Permanent link to this revision of the page">Permanent link</a>
 +
            </li>
 +
 
 +
 
 +
        <li id="privacy"><a href="/2014.igem.org:Privacy_policy" title="2014.igem.org:Privacy policy">Privacy policy</a></li>
 +
        <li id="disclaimer"><a href="/2014.igem.org:General_disclaimer" title="2014.igem.org:General disclaimer">Disclaimers</a></li>
 +
    </ul>
 +
</div> <!-- close footer -->
 +
    </div> <!-- close footer-box -->
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
<!-- Served in 0.086 secs. --></body>
-
</a></
+
</html>

Latest revision as of 07:50, 19 February 2015

Team:Dundee/Team - 2013.igem.org

 

Team:Sumbawagen/Team

From 2014.igem.org

iGEM Sumbawagen 2014 · Econey

Project Description

BBa_J04450 has a lac promoter and mRFP gene, thus E. coli harboring this plasmid will give red color when the bacteria were grown with lactose or IPTG. Oppositely, the expression of mRFP will be decreased when glucose was present in medium through a mechanism called catabolite repression. We use this phenomenon to measure the concentration of glucose in honey by calibrating the color of E. coli medium using android-based mobile phone. Creation of novel circuit which consists of constitutive promoter followed by either Adenylate Cyclase or IIAGlc genes, which placed downward lac-mRFP circuit, may affect the sensitivity of catabolite repression. We expect an engineered E. coli, which shows red color expression in different range of glucose concentration. By changing mRFP gene with amilCP, a blue fluorescent protein, we may obtain E. coli expressing either red or blue color when different honey added to the medium due to different glucose concentration.