Team:StanfordBrownSpelman/Modelling
From 2014.igem.org
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<h3><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Modelling">Double Optimizer</a></h3> | <h3><center><a href="https://2014.igem.org/Team:StanfordBrownSpelman/Modelling">Double Optimizer</a></h3> | ||
- | <h7><center>A utility for simultaneous codon | + | <h7><center>A utility for simultaneous codon and gene synthesis optimization</h7> |
<h6> | <h6> | ||
Gene synthesis as a tool for biological engineering presents both opportunities and challenges. One opportunity presented is the ability to optimize codon usage in a gene to match that of a host organism. Compared to traditional cloning methods, this can increase protein yields in the host organism by several fold. However, while there exist a large number of freely-usable programs that perform codon optimization, there is no guarantee that the sequences these programs provide will be able to be synthesized. Specifically, in the case of genes with repetitive amino acid sequences, these programs will often generate outputs that contain too many repeated short DNA sequences to be synthesized commercially. | Gene synthesis as a tool for biological engineering presents both opportunities and challenges. One opportunity presented is the ability to optimize codon usage in a gene to match that of a host organism. Compared to traditional cloning methods, this can increase protein yields in the host organism by several fold. However, while there exist a large number of freely-usable programs that perform codon optimization, there is no guarantee that the sequences these programs provide will be able to be synthesized. Specifically, in the case of genes with repetitive amino acid sequences, these programs will often generate outputs that contain too many repeated short DNA sequences to be synthesized commercially. |
Revision as of 18:08, 10 October 2014