Team:StanfordBrownSpelman/Cellulose Cross Linker

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Stanford–Brown–Spelman iGEM 2014 — Cellulose Acetate

Methods
Methods here.


Figure 1. Figure caption here.
Results
Our initial approach was to include two identical cellulose-binding domains on either side of the streptavidin domain. However, this led to numerous problems with molecular cloning due to the repetitive nature of the sequence. We changed our approach to using two cellulose-binding domains with different sequences. This allowed us to successfully conduct the molecular cloning.
References
1. M Linder and T T Teeri (1996) The cellulose-binding domain of the major cellobiohydrolase of Trichoderma reesei exhibits true reversibility and a high exchange rate on crystalline cellulose. PNAS. 55. PMID: 24136966.
Additional Information
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